尿苷

酶法大量合成~（13）C标记尿苷二磷酸葡萄糖

Enzymatic Synthesis of Large Amounts of ~ ( 13 ) C-Labeled Uridine Diphosphoglucose

酒精调节尿苷二磷酸葡萄糖醛酸基转移酶mRNA的表达

The expression of uridine diphosphate glucuronosyltransferase mRNA regulated by alcohol

HPLC同时测定人尿中假尿苷次黄嘌呤含量

Determination on Concentration of Pseudouridine and Hypoxanthine in Human Serum by HPLC

HPLC法测定血浆中阿糖胞苷及阿糖尿苷浓度

Determination of cytarabine and uracil arabinoside in plasma by HPLC

用~3H-尿苷离体掺入实验研究兔腹腔巨噬细胞在识别抗原过程中的RNA合成。

RNA synthesis during antigen recognition in rabbit peritoneal macrophage was investigated by incorporation of 3H-uridine in vitro .

肺癌患者血清、尿中假尿苷浓度分别显著高于肺内良性占位性病变、肺部感染患者和健康对照者（P均＜0.001）。

The levels of serum and urinary pseudouridine in the patients with lung cancer were significantly higher than that in the patients with pulmonary benign space occupying lesion , pulmonary infectious diseases and in normal controls respectively .

产氨短杆菌Corynebacteriumammoniagenes转化乳清酸（OA）生成尿苷酸（UMP）的研究

Research of Conversion of Orotic Acid to Uridine-5-monophosphate by Corynebacterium Ammoniagenes

方法：分别用[~3H]胸苷、[~（14）C]尿苷和[~3H]酪氨酸标记测定DNA、RNA和蛋白质合成；

METHODS : DNA , RNA , and protein synthesis were measured by incorporation of [ 3H ] thymidine , [ 14C ] uridine , and [ 3H ] tyrosine , respectively .

流加底物dU对酶反应转化率无显著影响，表明该酶促反应为非2′－脱氧尿苷底物抑制型；

In addition , there was not substrate dU inhibition in enzyme reaction because the obvious change of conversion rate was not observed in dU fed batch process .

方法从新生儿脐带血分离出单个核细胞，体外培养并予5溴脱氧嘧啶尿苷（Brdu）标记48h。

Methods The mononuclear cells abstracted from cord blood of infants were cultured in vitro and marked with5bromodeoxyuridine ( Brdu ) for48 h.

目的：建立测定阿糖胞苷（Ara－C）及其代谢产物阿糖尿苷（Ara－U）血药浓度的方法，并用于5例急性髓细胞白血病（AML）患者血药浓度监测。

OBJECTIVE : To develop a method for the determination of Cytarabine ( AraC ) and its metabolite ( uracil arabinoside , Ara-U ) in plasma of AML patients .

采用多聚尿苷酸-琼脂糖柱层析，从总的RNA中分离到88μg总的mRNA，免网织红细胞溶胶体外翻译体系产物表明，分离的mRNA具有生物功能。

Applied poly ( U ) - sepharose column , 88 μ g total mRNA was got from total RNA . The in vitro products for rabbit reticulocyte lysate translation system showed that isolated mRNA possessed biological function .

饲养九周后终止试验，小鼠植入渗透泵灌注溴脱氧尿苷（Brdu）72小时。

Nine weeks after the dietary intervention , the animals were implanted with osmotic pumps for 72 hours to infuse bromodeoxyuridine ( BrdU ) .

方法采用5-溴-2′-脱氧尿苷结合的小鼠局部淋巴结细胞免疫化学方法和豚鼠局部封闭涂皮试验（Buehlertest）分别检测2,4-二硝基氯代苯和凡士林的致敏性。

Methods The murine local lymph node assay based on 5-bromo-2 ′ - deoxyuridine ( BrdU ) incorporation and Buehler test were used to test 2,4-dinitrochlorobenzene ( DNCB ) and geoline allergenicity .

病变机理是过量D－Galn入肝代替了尿苷二磷酸葡萄糖的代谢途径所引起的。

Pathogenesis were due to D-Galn substitute for uridine diphosphoglucose ( UDPG ) way of metabolism .

以HPLC与LC/MS联合定性香菇中5'-核苷酸为5'-肌苷酸，5'-鸟苷酸，5'-尿苷酸和5'-腺苷酸。

The 5 ' - nucleotide in lentinus edodes were identified as 5 ' - inosine monophosphate , 5 ' - guanosine monophosphate , 5 ' - uridine monophosphate and 5 ' - adenosine monophosphate by using HPLC and LC / MS.

用5μmol/L的5溴2脱氧尿苷（BrdU）对培养的hMSCs进行连续培养标记。抗氯霉素单克隆抗体杂交瘤细胞株的筛选及金标试纸检测方法的建立

HMSCs were labeled with BrdU . Filtration of hybridoma lines of monoclonal antibody and development of colloidal gold - labeled strip for rapid detection of chloramphenicol

采用单因素考察和均匀设计法U6（6~3）结合，以腺苷、鸟苷和尿苷含量为指标，优化了冬虫夏草的提取方法；

Determination of Nucleosides and their base in Cordyceps sinensis By using adenosine , guanosine and uridine as quality control criteria , extraction process of Cordyceps sinensis had been optimized with the aid of the uniform design U6 ( 63 ) .

纤维素合成酶和UDPG（二磷酸尿苷葡萄糖）焦磷酸化酶是纤维素合成过程中的关键酶。

Cellulose synase and UDPG pyrophosphorylase are the key enzymes during the biosynthesis of bacterial cellulose .

建立了一种经济、简便的一步酶法，大量合成尿苷二磷酸葡萄糖（UDPG）。

The authors set up a one-step enzymatic method to synthesize UDPG in much higher yields .

Apocynaceae系细胞尿苷二磷酸葡萄糖焦磷酸化酶的纯化和表征黄芪毛状根中尿苷二磷酸葡萄糖焦磷酸化酶活性与多糖含量关系的研究

Study on Relationship between Uridine Diphosphate Glucose Pyrophosphorylase Activity and Polysaccharide Content in Hairy Root of Astragalus membranaceus

目的：探讨肝硬化及肝纤维化对尿苷二磷酸葡萄糖醛酸基转移酶（UGT）表达水平的影响。

AIM : To investigate whether cirrhosis affects the expression of hepatic glucuronosyltransferase ( UGT ) isoforms and its clinical significance .

目的研究氟尿苷（FUDR）在大鼠血清及子宫组织内的分布、代谢特征并进一步探讨FUDR与氟尿嘧啶（5FU）转化关系。

OBJECTIVE To study the distribution and metabolism of FUDR in rats , and to explore the in vitro conversion from FUDR to 5FU .

本研究探讨以尿苷二磷酸半乳糖（UDP-Gal）为添加剂的血小板冷藏保存方法。

This study was aimed to investigate the method to cold-store platelets with uridine diphosphate galactose ( UDP-Gal ) .

目的：探讨尿中假尿苷（Pseudouridine简称Ψ）含量对宫颈癌、卵巢癌的诊断价值。

Objective : Study the value of measure of pseudouridine (Ψ) in the diagnosis of cervical and ovarian cancer .

为了解残留尿苷酶对dUTP掺入CDNAPCR产物的破坏作用。采用PAGE和DNA-EIA杂交技术检测抗污染效果和DNA杂交百分率。

To explore the destructive effect of residual uracil DNA glycosylase on dUTP incorporation to the cDNA PCR products , PAGE and DNA-EIA hybridization technique were applied to detect the efficiency of anti-contamination and the rate of DNA hybridization .

5-氟-2′-脱氧尿苷（FUdR）是一种氟代脱氧核苷类似物，被广泛用于治疗各种肿瘤。

Floxuridine ( FUdR ) is a fluorinated deoxynucleoside analog , which has been used extensively in the treatment of a variety of tumors .

方法取孕龄15～18d胎鼠脑组织，分离获得神经干细胞，在体外培养、扩增，并用5溴2脱氧尿苷（BrdU）标记。

Methods Neural stem cells were isolated from the brain tissue of fetal rats ( P15-18 ) and cultured , expanded in vitro , marked by BrdU .

了解术前脱氧氟尿苷口服化疗对胃癌组织血小板衍化内皮细胞生长因子（PD-ECGF）表达的影响及意义。

Objective To investigate the relationship of the expression of platelet-derived endothelial cell growth factor ( PD-ECGF ) and survivin to apoptosis in pancreatic cancer .

利用产气肠杆菌EAM-Z1转化合成5-氟尿苷的转化率影响因素的研究

The Studies of the Factors Affecting the Conversion Rate of 5-FUR by Enterobacter Aerogenes EAM-Z1