导法

在杨树的转化过程中，DNA直接转移法和农杆菌介导法都有应用，但后者较为常用。

However , the application of Agrobacterium _ mediated DNA transfer was more common than that of direct DNA transfer .

本文以农杆菌介导法将Bt基因导入受体白交系18（红）所获得的转基因再生植株作为主要研究材料。

In this text , we used transgenic Bt maize by Agrobacterium as the main research materials .

农杆菌介导法获得籼稻温敏核雄性不育系转Bt基因植株

Production of Bt-transgenic Plants Indica Thermo-sensitive Genic Male Sterile ( TGMS ) Rice Line Using Agrobacterium-mediated Method

利用离子束介导法将大豆DNA导入小麦，经过连续4代田间筛选和蛋白含量测定，获得高蛋白变异株系。

Wheat was transformed with soybean DNA through particle beam mediation and its high-protein offspring plants were obtained through field selections and protein determinations in the four consecutive generations .

该研究小组还利用新的探针开发了一种新的蛋白标记方法，称为探针的酶介导法（PRIME）。

The group also developed a new protein labeling method , called probe incorporation mediated by enzymes ( PRIME ), which utilizes the new probes .

通过对基因枪介导法转化的烟草叶片及洋葱表皮细胞中GFP基因的瞬时表达测定表明，这一表达系统在光照条件下可显著增强GFP基因的表达。

The results indicated that this expression system has strengthened the transient expression of GFP .

通过农杆菌介导法将口蹄疫结构蛋白全长基因P1导入大豆的研究

Transformation of structure protein gene P1 of foot and mouth disease virus into soybean via Agrobacterium-mediated

本研究以抗病基因GO和抗病毒基因PAP为目的基因，研究了农杆菌介导法和基因枪法转基因定向改良嘎拉苹果抗病性。

The objective of the present study was to transfer the resistance gene GO and gene PAP to Gala apple by using particle gun and Agrobacterium-mediated methods .

采用农杆菌介导法成功地将反义PG基因导入新疆加工番茄（品种代号：98-75）。

Agrobacterium-mediated transformation had been used to introduce the Anti-PG gene into processing tomato ( cv . 98-75 ) .

载体被精子介导法导入家蚕受精卵，经绿色荧光蛋白观察和G418筛选，制得转基因家蚕。

The vector was transferred into silkworm eggs by sperm-mediated gene transfer .

然后采用农杆菌介导法转化生菜（皱叶生菜），获得卡那霉素抗性植株169株，对转基因T0代植株进行PCR检测，有35株为阳性，阳性率为20.7%；

Tumefaciens and 169 kanamycin resistant strains were isolated . PCR analysis of this generation revealed that 35 of 169 were positive , and the ratio was 20.7 % .

果树基因转化方法主要包括农杆菌介导法、电激法、基因枪法、PEG转化法等。

The methods include using Agrobacterium mediated transformation , electroporation , gene gun bombardment and polyethyleneglycol ( PEG ) mediated direct DNA transfer .

实验结果表明：①在农杆菌介导法获得的82株G418抗性植株中有75株PCR结果呈阳性；

The results showed as follow . ① 75 of 82 G418-resistant plants obtained by Agrobacterium-mediated transformation , were PCR positive lines .

方法：采用腺病毒介导法将野生型P53基因（Ad-P53）转染至人瘢痕疙瘩成纤维细胞中；将来源于瘢痕疙瘩组织的成纤维细胞随机分成二组。

Method : Fibroblasts derived from keloid tissue were transfected by adenovirus - wt - P53 gene .

利用农杆菌介导法获得转ipt基因半夏植株

Transgenic Pinellia ternate ( Thunb . ) Breit Plants Containing ipt Obtained by Agrobacterium-Mediated Transformation

为了获得抗真菌病的番茄材料，采用农杆菌介导法将细菌几丁质酶基因转化番茄子叶，获得了抗卡那霉素的转化植株，PCR检测初步证明细菌几丁质酶基因已整合到番茄的基因组。

For getting tomato that resists fungi diseases , a chitinase chiA gene from Serratia marcescens was transformed into tomato cotyledons by using Agrobacterium tumefaciens mediated method and transgenic plants with kanamycin resistance were obtained .

阐述了化学导入法（PEG）、电穿孔法、注射法，粒子轰击法、花粉管萌导法及使用其它载体系统的成功实例。

The direct gene transfer systems involved the chemical method ( PEG ), electroporation , microinjection , particle bombardment , pollen-mediated gene transfer and other systems using various vectors .

利用农杆菌介导法将构建好的PL基因反义表达载体和PL与β-Gal基因双价反义表达载体分别转化番木瓜，获得了抗性体胚。

PL antisense expression vector and PL ,β - Gal antisense expression vector were transformed into papaya mediated by Agrobacterium tumefaciens co-transformation , and the kan-resistant somatic embryo were obtained .

采用动态流导法，在分子流状态和不同温度下，测量了PP膜的放气率和平衡压强并研究了其放气特性。

The outgassing rate and its eguilibrium pressure are measured by the dynamic conductance method at the molecular flow condition and different temperatures for the polypropylene film . Its vacuum characteristics is discussed in this paper .

通过农杆菌介导法和针刺法，首次将蜘蛛神经毒蛋白杀虫肽基因Toxin和Bt基因导入复叶槭，经分子生物学检测，获得了复叶槭抗虫转基因愈伤组织和转基因植株。

The Toxin gene and Bt gene were first transformed into Acer negundo by Agrobacterium-mediated method and needle-sticking method . Transgenic calli and transgenic plants were obtained by PCR and PCR-Southern hybridization analysis .

用它构建了带动报告基因Gus表达的双元载体，并用农杆菌介导法转化水稻得到了转基因植株。

The cloned promoter has been used in construction of Agrobacterium binary vectors with Gus reporter gene , and several transformed plants were obtained through Agrobacterium mediated transformation .

用脂质体介导法将pRevTRE/HSVtk/teton导入乳腺癌细胞株（MCF7）。

Transfection of pRevTRE / HSVtk / Tet-On into MCF-7 cells was performed by using lipofectin .

采用脂质体介导法将构建的重组质粒pVVP3IL-18HN体外转染Hep-2细胞，运用MTT法检测不同质粒浓度、不同作用时间，该重组质粒对喉癌细胞Hep-2的杀伤率。

The recombinant plasmid was introduced into Hep-2 cells by liposome , then suppression rate of Hep-2 of different time and different quantity was calculated according to MTT results .

采用根癌农杆菌介导法将油菜CBF1基因转入拟南芥，筛选抗性转基因拟南芥植株，进行PCR检测和GUS染色。

CBF1 gene from rape was transformed into Arabidopsis thaliana by Agrobacterium tumefaciens-mediated method to screen its transgenic plants with resistance and conduct PCR detection and GUS staining .

将其与GUS报告基因融合在一起，构建了植物表达载体，并由农杆菌介导法导入水稻品种‘中花11’中。

It was fused with GUS reporter gene to construct a plant expression vector and the vector was transformed into Zhonghua 11 , a rice variety , by Agrobacterium meditation .

【方法】利用农杆菌介导法转化小麦幼胚愈伤组织，将SacB基因导入4个小麦品种，并对转化植株进行PCR和Southern杂交检测。

【 Method 】 Using wheat immature embryo calli as explants , SacB gene was transferred into four wheat cultivars by Agrobacterium-mediated transformation . Transformed plants were detected by PCR and Southern blotting .

以HPT基因作为筛选标记基因，潮霉素作为筛选剂，通过农杆菌介导法将GFP基因导入棉花细胞并得到再生植株。

Employing HPT as a selection marker gene and hygromycin as a selection agent , The GFP gene was transformed into cotton via agrobacterium and regeneration plants were acquired .

用精子介导法将转基因载体导入家蚕卵内，通过GFP荧光和G418抗性双重筛选，结合PCR、点杂交等分子生物学鉴定获得了转基因家蚕。

The vector was transferred into Bombyx mori eggs by sperm-mediated gene transferring methods . Screened with GFP and antibiotics G418 , combined identification of PCR and Dot hybridization , transformed silkworms were obtained .

用根癌农杆菌介导法将所有载体转化水稻，GUS组织化学分析和荧光测定结果表明：（1）培养基中的2,4-D强烈抑制gus基因在愈伤组织阶段的表达；

The results of histochemical GUS staining and fluorometric measurements showed : ( 1 ) Expression of gus gene was intensively inhibited by 2,4-D in the culture medium at callus stage ;

以GUS为报告基因，利用该启动子构建表达载体，农杆菌介导法转化番茄，获得了转基因番茄植株，研究该调控序列的组织表达特异性。

To investigate the tissue expression pattern of the cloned regulatory sequence . an expression vector containing this sequence fused with GUS was constructed for transformation into tomato by using agrobacterium-mediated method .