寡核苷酸芯片

寡核苷酸芯片用于HLA-A基因分型的研究

HLA-A genotyping by oligonucleotide arrays

目的：利用寡核苷酸芯片检测SARS病毒。

Objective : To detect SARS virus by hybridization in oligonucleotide array .

基于寡核苷酸芯片的β-地中海贫血DNA甲基化的研究

DNA methylation in β - thalassemia by oligonucleotide microarray

在分类上，基因芯片基本上可分为cDNA芯片和寡核苷酸芯片两种。

DNA chips can be classified into two categories : cDNA chips and oligo chips .

检测及鉴定RoundUpReady转基因大豆寡核苷酸芯片的制备

Preparation of Oligonucleotide Microarray for Detecting and Identifying Roundup Ready Soybean

结论：寡核苷酸芯片适于快速准确、平行大量地检测SARS病毒。

Conclusion : Oligonucleotide array is a fast way to detect SARS virus .

应用多重RT-PCR结合寡核苷酸芯片方法检测儿童白血病常见融合基因

Identification of Gene Rearrangements in Childhood Leukemia by Using a Multiplex Polymerase Chain Reaction-Microarray Approach

利用寡核苷酸芯片（oligonucleotidearray）对兽医临床常见病原菌耐药性检测进行了初步研究。

Detection of the antimicrobial resistance in common veterinary pathogen by using oligonucleotide array was studied .

利用寡核苷酸芯片对RoundUpReady转基因大豆检测及鉴定技术的研究

Study on Detection and Identification of Roundup Ready Soybean Using Oligonucleotide Microarray

从Internet、国内外文献中查询了50个水稻花序的相关基因，制备成水稻花序相关基因的寡核苷酸芯片。

In this paper we chose 50 rice inflorescence genes from Internet , references . Rice oligonucleotide microarray was prepared by printing the target rice inflorescence genes oligonucleotide .

寡核苷酸芯片检测肺癌患者痰标本中p53点突变

Clinical study on detection of p53 gene hot-spot mutations in sputum sample of lung cancer using oligonucleotide microarray

应用寡核苷酸芯片并行检测肝细胞癌p53基因突变类型

Detecting in parallel point mutation pattern of p53 gene in hepatic cellular cancer by the technique of oligonucleotide chips

16Srdna寡核苷酸芯片鉴定致病菌的初步研究

Development of 16S rDNA oligonucleotide microarray for identification of pathogenic bacteria

结论采用寡核苷酸芯片技术对HLA基因分型是种好的方法，具有测速快、成本低、高通量的优点。

Conclusion HLA A genotyping by oligonucleotide array is a good method with advantage of high speed , low cost and high flux .

bcr-abl基因检测寡核苷酸芯片的制备及分析

Identification bcr abl Fusion Gene in Leukemia Cells with Oligonucleotide Microarray

结果：SARS阳性样品与寡核苷酸芯片杂交后出现阳性杂交信号，具有不同二级结构的寡核苷酸探针杂交信号强度不一。

Results : Positive SARS samples were detected by oligonucleotide array and fluorescence signal intensity was highly related to the probe 's second structure .

基于23Srdna基因的病原细菌通用检测寡核苷酸芯片

A universal detection system for pathogenic bacteria based on 23S rDNA gene-targeted oligonucleotides chips

采用不对称PCR和间接荧光标记技术进行单链DNA扩增和荧光标记，标记样品与寡核苷酸芯片杂交后，进行芯片清洗、扫描及结果分析。

By means of asymmetric PCR and indirect fluorescent-labeling and technology to amplify and label the samples , they were hybridized with the oligonucleotide microarrays , followed by washing , scanning and analysis .

利用该寡核苷酸芯片检测方法对32例经临床实验室常规培养鉴定方法确定为细菌感染的CSF标本进行鉴定。

A total of 32 CSF samples , which were verified the bacterial infection by the routine method , were tested by this methods .

寡核苷酸芯片（oligonucleotidechip）技术近年来发展迅速，其制备方法随着应用目的不同而多种多样，其中芯片片基的表面处理是制备芯片的关键技术之一。

Oligonucleotide chip has developed rapidly and its method is diverse according to the purpose . The surface treatment of the chips is one of the key techniques .

RD标记方法是一种有用的低背景信号的高密度长链寡核苷酸芯片样本标记方法。

It is concluded that the RD-PCR is a potential and useful sample labeling method with lower background signal intensities for studying high-density long oligonucleotide microarray .

方法采用果蝇寿命实验，观察EF延长果蝇寿命的效果；采用果蝇全基因组寡核苷酸芯片研究EF延缓衰老的机制。

Methods The effect of EF on longevity of drosophila was detected by lifespan test , and the mechanisms were probed using oligonucleotide microarray .

方法分别制备卵巢癌原发灶和肝转移灶组织标本mRNA，线性扩增后与人类寡核苷酸芯片杂交，通过信号扫描、处理后获得两者的表达差异基因。

[ Method ] The mRNA of the tissues from the in situ site and hepatic metastasis were extracted respectively and hybridized with human oligo microarray after amplification , and the differentially expressed genes were obtained .

cDNA微阵列和寡核苷酸芯片等高通量检测技术的成熟应用，产生了大量的基因表达数据，其中包括静态数据和时序数据。

The mature application of high-throughput detection technology , such as cDNA microarray and oligonucleotide microarray , produced a large amount of gene expression data , including static data and time series data .

寡核苷酸芯片技术以其快速、高通量、大信息量检测等特点而应用广泛，尤其在对HLA-B分型方面显出很大优势。

The oligonucleotide chip technology displays the predominance by the celerity , high flux and tremendous information detection .

为建立细菌性生物恐怖剂和重要医学细菌的筛查方法，本研究旨在建立一种基于16Srdna寡核苷酸芯片的检测和鉴定常见致病菌的技术。

For the rapid and efficient detection of biological agents and clinical infectious bacteria , a 16S rDNA oligonucleotides microarray based detection and identification system was developed .

本方法将实验步骤进一步改进，增加额外的一轮体外转录，并结合Klenow酶标记技术来制备cDNA靶标和寡核苷酸芯片杂交。

In this work , the protocol was further optimized to hybridize oligonucleotide microarray by applying an addition round of amplification and a cDNA labeling approach with Klenow enzyme .

本研究建立的猪繁殖与呼吸综合征病毒、猪瘟病毒寡核苷酸芯片方法经初步应用表明，其在高致病性PRRS的鉴别诊断与猪瘟病毒基因分型中显示了一定的应用潜力。

The results of this study showed the promising application of the microarray for differential diagnosis of the highly pathogenic PRRS and classical PRRS and genotyping of CSFV .

目的初步研究限制性显示技术（RD-PCR）作为一种高密度长链寡核苷酸芯片样本标记方法对芯片杂交信号的影响。

Objective To investigate the value of restriction display PCR ( RD-PCR ) as a novel and expedient sample labeling method for high-density 60-mer oligonucleotide microarray .

应用人类全基因组寡核苷酸芯片筛查自身免疫性肝炎相关基因

Study on autoimmune hepatitis associated genes by human genome oligonucleotide microarray