完全抗原

同时蛋白质芯片检测结果显示，偶联物与OTAMcAb之间发生了特异性反应，提示完全抗原的存在。

Protein microarray analysis also showed that specific reaction occurred between OTA McAb and conjugate , suggesting the existence of complete antigen .

紫外扫描显示，偶联物与OVA的波型不同，可初步判断完全抗原的存在。

The UV scanning result showed that OVA and conjugate had different wave type . So it indicated the existence of complete antigen .

主要是通过电泳方法将完全抗原固定到修饰NC膜的金电极上。

The complete antigens were immobilized on NC membrane modified on a gold electrode by electrophoresis .

同时应用聚丙烯酰胺凝胶电泳、紫外分光光度法、红外光谱检测技术以及Quantityone凝胶分析软件计算偶联率等方法建立了人工完全抗原的表征体系。

Applying gel analysis software Quantity One , the calculation method of immunogen coupling rate was established , and the characterization system for the immunogen was also discussed .

方法：合成的BNP与牛血清白蛋白（Bovineserumalbumin，BSA）以碳化二乙胺（EDC）法偶联，制备BNP-BSA完全抗原。

Methods : The synthesized BNP was conjugated to bovine serum albumin ( BSA ) by EDC coupling procedure to form complete antigen ( BNP-BSA ) .

本研究分别以牛血清白蛋白（BSA）、卵清蛋白（OVA）为蛋白质载体，采用碳二亚胺法（CDI）将半抗原青霉素钠（PEN）分别与BSA、OVA偶联，合成了完全抗原PEN-EDC-BSA和PEN-EDC-OVA。

In this study penicillin hapten was respectively coupled with BSA and OVA to synthesize PEN-EDC-BSA and PEN-EDC-OVA complete antigen applying CDI .

结果用碳化二亚胺法成功地将微量半抗原OA与BSA和卵清白蛋白（OVA）制成了完全抗原OA-BSA和OA-OVA。

Results According to carbodiimide method , antigens of OA-BSA and OA-OVA are successfully prepared from minute amount hapten of OA .

方法将B型流感病毒为完全抗原免疫BALB/c小鼠，得到的脾细胞与SP2/0细胞融合，获得稳定分泌抗流感B单抗的杂交瘤细胞株，对抗体进行鉴定。

Methods Influenza B virus was used for the immunization of BALB / c mice . The hybridoma cell strains stably secreting McAbs against influenza B virus were obtained by fusion of murine splenocytes with SP2 / 0 plasmacytoma cells , and the secreted McAb was identified .

从偶联位点、偶联剂、载体蛋白和偶联步骤等分析出发，设计了微囊藻毒素-LR（Microcystin-LR，MC-LR）完全抗原的制备方法。

Based on the analysis of coupling location , coupling regent , carrying protein and coupling process , completed antigen of Microcystin-LR ( MC-LR ) was synthesized .

紫外扫描分析表明，T-2毒素与牛血清白蛋白的偶联比为6.66∶1、与卵清蛋白的偶联比为10.11∶1，表明此完全抗原能够用于免疫动物。

UV Spectrum analysis showed that the conjugation ratio of T-2 toxin to BSA was6.66 ∶ 1 and that to OVA was10.11 ∶ 1 , indicating this antigen could be used to immunize animals .

本课题的目的是设计正确的合成路线，优化合成工艺，制备CsA完全抗原。首先对CsA分子的化学结构进行修饰，引入活性基团；然后与蛋白载体相偶联，形成完全抗原。

The objective of this experiment is to design correct synthetic route , optimize synthesis technology , and synthesis the Immunogen of Cyclosporin A. First , modify its structure to introduce such active functional groups . Then couple with carrier protein or polypeptide forming a whole antigen .

通过对新西兰大白兔免疫自制的微囊藻毒素-LR（Microcystin-LR，MC-LR）完全抗原BSA-MC-LR，获得了质量较好的抗MC-LR的多克隆抗体，间接ELISA表明抗体的效价能达到1.5×105；

Good quality polyclonal antibody against Microcystin-LR ( MC-LR ) was obtained from the New Zealand rabbit immunized with the homemade complete antigen BSA-MC-LR . Indirect ELISA shows the titer of this antibody is more than 1.5 × 10 ~ 5 ;

以混合酸酐法将DES-MCPE与载体蛋白偶联形成完全抗原，并以紫外扫描分析了完全抗原的偶联比率。

The conjugates of DES-MCPE with proteins as complete antigens were prepared by the mixed anhydride method and the conjugation rate of them were analyzed by UV scanning .

微囊藻毒素-LR完全抗原的设计及制备

Synthesis of Complete Antigen of Microcystin-LR and Its Antibody Production

目的制备绿原酸完全抗原，对完全抗原进行鉴定。

Objective Characterizate the complete antigen of the chlorogenic acid .

盐酸克伦特罗完全抗原的合成及其偶联工艺的优化

Synthesis of Clenbuterol Hydrochloride Complete Antigen and Optimization of its Conjugation Conditions

海洋毒素大田软海绵酸完全抗原的制备与分析

Preparation and analysis of complete antigen for okadaic acid

阿莫西林完全抗原的合成与鉴定

Synthesis and identification of amoxicillin artificial antigens

从而了解到完全抗原只能用灭活金黄色葡萄球菌或者纯化的荚膜多糖结合蛋白质来实现。

In a word , the whole heat-killed staphylococcus aureus or carbohydrate-binding protein can be complete antigen .

氧氟沙星完全抗原的合成鉴定及其单克隆抗体的制备纯化

Synthesis and Identification of the Antigen of OFLX and the Production and Purification of Its Monoclonal Antibodies

免疫小鼠，以血清抗体滴度分析6种完全抗原的免疫原性差别。

The antibody titers in mice serum were determined which were immunized with six complete antigens , respectively .

本论文通过开发一个氯氨酮完全抗原，再用此抗原免疫老鼠，应用单克隆技术，开发一个氯氨酮单克隆抗体。

Our project is to develop a Ketamine antigen , them use this antigen to immunize mice , using monoclone technology to develop a Ketamine antibody .

本实验采用琥珀酸酐和碳二亚胺法将沙丁胺醇偶联到牛血清白蛋白和卵清白蛋白上，成为完全抗原Sal-HS-BSA和Sal-HS-OVA。纯化后进行检测，确认沙丁胺醇已经连接到蛋白质上。

Conjugating Sal to bovine serum albumin ( BSA ) and ovi albumin ( OVA ) by succinic anhydride and carbodiimide transformed Sal , an half antigen , into complete antigen .

以结合比为16：1的完全抗原免疫新西兰白兔，获得了效价达到6.55×106兔抗血清。

Added amount of EDC controlled in 6 ~ 9mg . The New Zealand rabbits were immunized with complete antigen of combined ratio of 16:1 , the antiserum 6.55 × 106 was obtained .

肿瘤RNA转染DC可以实现完全性抗原的加工递呈，诱导强烈的肿瘤特异性CTL反应。

Tumor RNA transfection of DCs can fulfill the uptake and presentation process of total rumor antigens , and can induce potent antitumor immunity .

然而，一个完全没有Rh抗原的人是特别难以找到的。

Still , it 's extremely extraordinary for someone to not have a single Rh antigen .

CD154／CD40信号转导途径是介导CD4~+T淋巴细胞的辅助作用与诱导树突状细胞完全成熟并获得抗原递呈与免疫刺激能力的重要途径。

The help of the CD4 + T lymphocytes mediated by CD154 / CD40 signal pathway leads to the induction of fully mature DCs having the ability to present antigens and stimulate CD8 + CTLs .

模型组和药物治疗组用加入弗氏完全佐剂的颌下腺抗原多点免疫，所有接受不同处理的大鼠实验时间均为21天。

Then Lewis rats of SS model and drug therapeutic groups were sensitized on multiple spots with SMG antigen in complete Freund adjuvant ( CFA ) . These rats had received different treatment for 21 days .