双标记

免疫荧光双标记显示部分细胞为βAPPGFAP双标阳性细胞。

Fluorescent double labeling showed that the β - APP positive cells were co-localized with GFAP positive cells .

目的：为了观察下丘脑外侧核乙酰胆碱酯酶（AchE）阳性神经元的传入纤维联系.方法：采用HRP与AchE相结合的双标记方法，对17只大鼠下丘脑外侧核进行了逆行追踪研究。

The afferent projections of AchE positive neurons of the lateral hypothalamic nucleus were studied in 17 rats with HRP-AchE double labeling techniques .

~3H、~（14）C双标记测量采用迭代法计算结果

~ 3H , ~ ( 14 ) C-double-labelling measurement by interactive calculation method

用双标记时间分辨荧光免疫法同时检测黄曲霉毒素B1和赭曲霉毒素A

Simultaneous detection of aflatoxin B_1 and ochratoxin A by the dual-label time-resolved fluoroimmunoassay

应用免疫荧光双标记观察APP表达阳性细胞。

Fluorescent double - labeling method was used in the study .

慢性乙型肝炎肝内HBVdna和HBsAg的双标记定位研究

Localization of HBV DNA and HBsAg in Liver with Chronic Hepatitis B by Double Labelled Method

方法：采用荧光双标记法，对32只SD大鼠皮质下结构向隔内、外侧核和斜角带核垂直支的分支投射进行逆行追踪。

Methods : With retrograde axonal transport of fluorescence double labelling methods in 32 rats .

~（14）C、~（15）N双标记秸秆对土壤微生物量碳、氮动态变化的影响

Influence of ~ ( 14 ) C and ~ ( 15 ) N labelled Straw Application on Dynamics of Soil Microbial Biomass C and N

方法OPN在肾脏组织蛋白和基因水平的表达分别应用免疫组化双标记及原位杂交技术。

Methods Double labelling immunostaining and in situ hybridization .

大鼠颌下腺GnRH及其受体的免疫组织化学双标记和年龄变化

Immunohistochemical double label and aged changes of GnRH and its receptor in submaxillary gland of rats

C-肽双标记液相IRMA方法的实验研究

Experimental Research of C-P Liquid Phase IRMA with Two Labeled Antibodies

C端带Myc和多聚组氨酸双标记的重组人β-防御素-2在COS-7细胞的转染表达

Expression of Recombinant Human β - Defensin-2 Gene with C Terminal of Double Marks of Myc and Poly-Histidine in Transfected COS-7 Cells

方法：荧光金（FG）逆行追踪与PKCγ免疫荧光组织化学染色相结合的双标记技术。

Methods : Fluoro gold ( FG ) retrograde tracing method combined with immunofluorescence histochemical staining for PKC γ were used .

用磷脂结合蛋白V/碘化丙啶（AnnexinV/PI）对K562细胞进行双标记后，在流式细胞仪下检测细胞凋亡率。

K562 cells were labelled with Annexin V / PI , and then the rate of apoptosis was measured by flow cytometry ;

本文报导了~3H和~（32）P、~3H和~（14）C双标记样品液体闪烁测量法，使用仪器为BeckmanLS8100和NE8312液体闪烁谱仪。

This paper described a method in which 3H and 32P , 3H and 14C dual labelled samples were counted by a Beckman LS 8100 or an NE 8312 liquid scintillation spectrometer .

在FG和CB双标记的神经元中，又有一部分（约385%）为FG/CB/Fos三标细胞。

About 38 5 % of FG / CB double labeled neurons were FG / CB / Fos triple labeled in the INV ;

用PRV和5－HT免疫组织化学双标记方法研究脑内5－HT能神经元对咽肌的神经支配及调控。

Innervation and control of 5 HT neurons to the pharyngeal muscle were studied using PRV and 5 HT immunohistochemical double labeling method .

PCNA+NF-160（或Chat、GABA、GAD）的免疫双标记结果显示，一些细胞的胞浆显示为棕色的同时细胞核显示为黑色。

After PCNA + NF-160 ( or Chat , GABA , GAD ) double label , some cells showed brown cell plasma and black nucleus .

用间期核双标记荧光原位杂交技术检测BCL2原癌基因易位

Detection of BCL2 translocation in an interphase neucleus using fluorescence in situ hybridization strategy

在胰腺内分泌部（胰岛）5-HT免疫反应细胞分布均匀，大部分胰岛细胞呈阳性5-HT样免疫反应，用相邻薄切片免疫双标记技术证明，胰岛内的5-HT免疫反应细胞主要是B细胞。

The majority of islets cells showed positive 5-HT immunostaining . The coexistence of 5-HT and insulin in the pancreatic islets was also proved with immunostaining of adjacent thin sections .

背根神经节降钙素基因相关肽阳性神经元周围突分支支配心和胃的研究&荧光素双标记与PAP结合法

Simultaneous innervation of heart and stomach by dichotomizing branch of peripheral process of CGRP immunoreactive dorsal root ganglion neurons & a triple labelling study

用免疫组织化学法，以邻片双标记技术研究了大鼠颌下腺内GnRH及GnRH受体的分布；并对不同发育阶段大鼠颌下腺内GnRH及其受体进行了原位定量分析。

By immunohistochemical method , the double label and aged changes of GnRH and its receptor in submaxillary gland of male rats were studied .

大鼠中缝核5-HT能神经元向嗅结节的投射-HRP法与免疫组化法双标记研究

A study on cells containing 5-HT project to THF olfactory tubercle from the raphe nuclei & a double label method of horseradish peroxidase and serotonin immunocytochemistry in rats

因此，在没有激光扫描共聚焦显微镜时，可用AdobePhotoshop7.0或imageJ图像处理软件将普通荧光显微镜采集的同视野两种单标记免疫荧光图像合成为保持原有特点、简便而清晰的双标记荧光图像。

Thus , in the absence of a confocal microscope , co-field double-stained immunofluorescence images can be obtained from single-stained images collected with a common fluorescence microscope using Adobe Photoshop 7.0 or Image J image-processing techniques .

作者用原位杂交和免疫组织化学PAP法双标记，在51例人肝硬变石蜡组织切片中检测HBVdna和HBsAg。

Hepatitis B virus DNA ( HBV DNA ) and HBsAg were detected in 51 human paraffin - embedded sections of liver cirrhosis by the double labelling technique of in situ hybridization and PAP method .

应用3H－TdR及14C－UR参入淋巴细胞的双标记方法，在体外培养条件下，研究多抗甲素对人淋巴细胞DNA及RNA合成功能的影响。

Through the double labeling way of 3H-TdR and 14C-UR adding to the lymphocyte , In vitro culture , was studied the synthetic function of human lymphocyte DNA and RNA .

方法分离纯化乳鼠心成纤维细胞，免疫荧光双标记技术进行了Vim和Mi的标记，激光扫描共聚焦显微镜观察。

METHODS The cardiac fibroblasts of rat underwent double labeling of Vim and Mi by immunofluorescence technique after isolation and purification , then were observed with laser scanning confocal microscope .

结果模型+G-CSF组再灌注后24h出现CD34/Brdu双标记，48h时双标记最为显著；

Results In the G-CSF treatment group , 24 hours after the focal cerebral ischemia / reperfusion injury , CD34 / Brdu dual labeled cells were found in the ischemia area ;

方法应用体外HSC培养技术，采用~3H-胸腺嘧啶核苷（~3H-TdR）掺入法测定HSC增殖；膜联蛋白（Annexin&V）/碘化丙啶（PI）双标记流式细胞术及透射电镜等方法测定HSC凋亡；

Methods 3H-thymidine incorporation , annexin-V / propidium iodide double-labeled flow cytometry ( FCM ) and transmission electron microscopy were employed to estimate the influence of RGDS on the proliferation and apoptosis of HSCs .

方法采用免疫组化单、双标记染色技术等，检测77例各型丁肝病人肝组织中HDAg、bcl2、bax和bak表达。

Methods Expressions of HDAg , bcl 2 , bax , and bak in liver specimens of 77 patients with hepatitis D were studied by immunohistochemical method .