包膜糖蛋白

人类疱疹病毒6B包膜糖蛋白gO在感染细胞中的表达与检测

Expression and Detection of Human Herpes Virus 6B Envelope Glycoprotein O

HSV-1包膜糖蛋白G原核表达克隆的构建和表达

Construction and expression of the prokaryotic expression clone of HSV-1 envelope glycoprotein G

HIV-1包膜糖蛋白V3环对大鼠海马长时程增强效应的影响

Effect on long-term potentiation by HIV-1 V_3 loop in rat hippocampus in vitro

庚型肝炎病毒包膜糖蛋白E2基因在昆虫细胞中的表达

Expression of E2 Glycoprotein Gene of Hepatitis G Virus in Insect Cells

HSV-1包膜糖蛋白G重组蛋白的纯化与抗原性分析

The purification of the prokaryotic expression clone of HSV-1 glycoprotein G and antigenic analysis

HCV包膜糖蛋白E2基因的克隆、蛋白表达及纯化

Cloning of HCV E2 gene and expression and purification of HCV envelope glycoprotein E2

融合His标签的HCV包膜糖蛋白E2的真核表达及意义

The expression of protein fused HCV envelope protein E2 with His tag and its implication

风疹病毒包膜糖蛋白E1酵母表达载体的构建

Construction of yeast expression vector of rubella virus glycoprotein E1 gene

风疹病毒包膜糖蛋白E1的真核表达及其生物学活性研究

Eucaryotic Expression and Biological Activities of Rubella Virus Glycoprotein E1

风疹病毒E2包膜糖蛋白亮氨酸突变对细胞融合的影响

Effects of mutations at the E2 protein leucine sites on specific membrane fusion in rubella virus

风疹病毒包膜糖蛋白E1中二硫键对其细胞融合活性的影响

Effects of Disulfide Bridges in Glycoprotein E1 on the Membrane Fusion Activity of Rubella Virus

结论成功利用昆虫细胞表达了HCV包膜糖蛋白，为HCV疫苗的研究奠定了基础。

Conclusion HCV gE is expressed successfully in insect cells , the study lay the foundation for further developing HCV vaccine .

结果表明本实验成功构建了汉滩病毒包膜糖蛋白G2的重组体。

The results showed that this experiment successfully constructed Hantaan virus en-membraned glucoprotein G2 recombinant .

结论HHV-6B包膜糖蛋白gO和gL为病毒的晚期蛋白。

[ Conclusion ] HHV-6B enveloped gO and gL behave as late proteins of virus .

汉坦病毒包膜糖蛋白G2重组腺病毒的构建及其在Hela细胞中的表达

Construction of a recombinant adenovirus carrying the Hantaan virus glycoprotein G2 gene and its expression in Hela cells

人免疫缺陷病毒Ⅰ型包膜糖蛋白gp120基因在大肠杆菌中的高效表达

High-level expression of human immunodeficiency virus type 1 gp120 protein in E.coli

构成病毒颗粒的结构蛋白包括核心蛋白和包膜糖蛋白E1和E2。

The structural proteins , which form the viral particle , include the core protein and the envelope glycoproteins E1 and E2 .

目的构建V3环缺失的HIV1ADA株包膜糖蛋白表达体系。

Objective To construct an expression vector bearing HIV 1 ADA env with V3 loop deletion .

结论成功构建了V3环缺失的HIV1ADA株包膜糖蛋白的表达载体。

Conclusion Expression vector of HIV 1 ADA env with V3 loop deletion is constructed successfully .

KSHV包膜糖蛋白编码基因K8.1、K8.1A和gL的克隆及真核表达

Cloning of envelope glycoprotein K8.1 ﹑ K8.1A and gL gene of KSHV and eukaryotic expression

HCMV病毒感染细胞的过程是从病毒包膜糖蛋白与靶细胞膜表面受体结合开始的，结合后HCMV以膜融合或受体介导的内吞机制进入细胞。

HCMV infection starts from the viral envelope glycoprotein binding with the cell surface receptor . Then HCMV enters cells by membrane fusion or receptor-mediated endocytosis .

尽管RV的主要三种结构蛋白&包膜糖蛋白E1、E2和衣壳蛋白C都有着较高的免疫原性，在人体内可诱发强烈的抗体应答。

The main three RV structural proteins - the envelope glycoprotein E1 , E2 and capsid protein C have higher immunogenicity in the human body , and can induce a strong antibody response .

在昆虫细胞中表达丙型肝炎病毒（HCV）包膜糖蛋白E1和E2，将该蛋白应用于丙型肝炎患儿血清抗体的检测。

To detect the serum antibodies of children with hepatitis C using the envelope El and E2 proteins expressed by hepatitis C virus ( HCV ) E protein gene in insect cells .

人类疱疹病毒8型包膜糖蛋白编码基因K8.1的分离、克隆及在大肠杆菌中的表达

Isolation and Cloning of Envelope Glycoprotein K8.1 Gene of Human Herpesvirus-8 and Its Expression in E.coli

构建单纯疱疹病毒2型包膜糖蛋白D成熟肽基因毕赤酵母表达载体，并对序列进行分析，为进行高抗原性的真核表达重组gD蛋白奠定基础。

To construct Pichia pastoris expression vector of herpes simplex virus type 2 ( HSV-2 ) envelope glycoprotein D mature peptide gene , then analyze the gene sequences for expressing the recombinant gD .

针对第一个问题，研究人员通过分析当前已经找到的HIV广谱中和抗体，发现这些抗体对应的病毒抗原表位主要分布在HIV包膜糖蛋白gp120/gp41上的一些保守位点。

For the first issue , previous research works have shown that most of the viral antigen epitopes corresponding to these antibodies were located in some of the conserved sites on envelope protein gpl20 / gp41of HIV by analyzing known neutralizing antibodies .

克隆了伪狂犬病病毒鄂A株编码包膜糖蛋白gD的基因并进行了序列测定，与国外报道的Rice株相比，其核苷酸序列具有98%的同源性，推导氨基酸序列同源性为97%。

The envelope glycoprotein gD gene of pseudorabies virus Ea strain was cloned via PCR technique . Sequence analysis displayed 98 % nucleotide sequence homology and 97 % deduced amino acid sequence homology between our cloned gD gene and PRV Rice strain gD gene .

目前研究表明，HCMV的复制和感染需要依赖其病毒的包膜糖蛋白，后者在识别宿主细胞膜受体、吸附和穿入宿主细胞、病毒的致病力以及诱导宿主反应等方面起重要作用。

Studies have shown that HCMV envelope glycoprotein is necessary for HCMV replication and infection and play an important role in in the host cell receptor recognition , absorption and penetration of host cells .

目的建立风疹病毒包膜糖蛋白E1的克隆载体；研究E1基因变异情况，并对其序列进行系统发生树分析。

Objectives To construct the cloning vector of glycoprotein E1 gene of rubella virus , to study the mutation of glycoprotein E1 gene , and to analyze the sequence of E1 gene by the phylogenetic tree .

方法分别设计包膜糖蛋白两端及V3环两端的两对引物，采用重叠延伸剪接法，进行HIV1ADA株包膜糖蛋白V3环缺失体的构建。

Methods Splicing by overlap extension ( SOE ) was used for deletion of HIV 1 ADA V3 loop . Two pairs of primers were designed and overlap PCR was taken placed .