前病毒

猴泡沫病毒SFV-1前病毒pol基因克隆及序列分析

Clone and Sequence Analysis of the pol Gene of Simian Foamy Virus Type 1 Provirus

结果8例患者的前病毒DNA水平介于0508～2210拷贝数/μgDNA之间，其复制和整合水平存在差异。

Results The levels of the provirus of the 8 patients ranged from 0 508 2 210 copies / μ g DNA , there were differences in the levels of replication and integration .

同时提取前病毒DNA，对外膜基因进行序列分析。

The env gene sequences form proviral DNA were analyzed by GCG software .

差别聚合酶链反应定量检测艾滋病患者外周血前病毒DNA水平

Differential Polymerase Chain Reaction for Quantitation of HIV DNA in Peripheral Blood Mononuclear Cell of AIDS Patients

一种简便、快速从福尔马林固定石蜡包埋组织块提取HIV前病毒DNA的方法

A Simple and Rapid Method for Extracting DNA from Formalin Fixed Tissue Embeded in Paraffin

用聚合酶链反应检测T细胞白血病／淋巴瘤中HTLV－I前病毒DNA

Detection of htlv - ⅰ proviral DNA from patients with T-cell leukemia / lymphoma by polymerase chain reaction

山羊关节炎&脑炎前病毒的PCR检测

Detection of Goat Arthritis-encephalitis Virus by PCR

NestedPCR在检测牛白血病病毒前病毒中的应用

Rapid Detection of Proviral DNA of Bovine Leukaemia Virus Using Nested PCR

结论PCR扩增HIV前病毒保守区多个基因片断，具有较高的灵敏度和特异度，方法简单，可以应用到HIV感染的早期诊断中。

Conclusion This method is highly sensitive and specific an can be used for early diagnosis of HIV-1 infection .

绵羊肺腺瘤病毒NM株前病毒gag基因的克隆与序列分析

Cloning and Sequencing Analysis gag Gene of Jaagsiekte Sheep Retrovirus Inner Mongolia Strain

马传染性贫血病毒弱毒株感染驴胎皮肤细胞（FDD）的前病毒DNA整合动态

Integration Dynamics of proviral DNA in fetal donkey dermal cell infected by attenuated strain of equine infectious anemia virus

方法：从HIV-1抗体阳性标本中提取HIV前病毒DNA，使用HIV-1型共用引物进行PCR扩增。

Methods : Proviral DNA extracted from the HIV-1 positive specimen was amplified by PCR with the shared primer of HIV-1 type .

中国东北地区HTLV-1的前病毒DNA测定及其意义

Determination and significance of retrovirus DNA of HTLV - 1 in northeast China region

全血DNA滤纸片中HIV1前病毒基因的PCR检测

Detection of proviral gene of human immunodeficiency virus type 1 from whole blood DNA on filter paper

目的研究干血斑（DBS）样本用于人类免疫缺陷病毒1型（HIV-1）感染前病毒DNA基因诊断的可行性。

Objective To assess the feasibility of detection of HIV-1 proviral DNA on Dried Blood Spot ( DBS ) samples by nested-PCR assay .

目的了解血清抗－HIV阴性的静脉药瘾者肝组织是否存在前病毒HIVDNA。

Objective The study was designed to investigate whether there existed provirus HIV DNA in the liver tissues of intravenous drug users ( IVDU ) with seronegative results of anti HIV test .

对马传染性贫血病毒阿根廷代表毒株前病毒gag和gp90基因核苷酸序列的动态检测结果表明，马传贫病毒在马体整合为前病毒之后比较稳定，这就为建立PCR鉴别诊断方法提供了依据。

The study showed that provirus was very steady after EIAV virus integrating into the cell genome , it offer a credible basis for PCR assay of EIAV .

建立DNA-PCR方法，检测SIV感染猴外周血淋巴细胞（PBMCs）中的前病毒DNA。

To develop a DNA-PCR technique for measurement of proviral DNA load in peripheral blood mononuclear cells ( PBMCs ) .

结果该法在猪肝脏组织中可检测出PERV前病毒序列；

Results Proviral PERV sequences were detected in liver tissue in pig .

方法检测CTCL患者血清中的HTLVⅠ抗体以及患者外周血细胞和活检标本中的前病毒DNA。

Methods Detecting antibodies to HTLV ? ⅰ in serum of patients with CTCL and testing pre virus DNA of HTLV ?

目的：探讨病毒学因素对原位肝移植（OLT）后乙肝病毒再感染的影响（包括术前病毒含量、HBVs基因变异）。

Objective : To make an inquiry into the relationship between virological factors ( HBV DNA quantity pre-OLT and HBV S gene mutation ) and HBV reinfection after OLT .

在感染后第6天，整合的前病毒的量达到高峰，其含量与病毒的致细胞病变作用（CPE）相对应。

The quantity of integrated proviral DNA reached to the highest level on day 6 postinoculation , and was correlated with the cytopathic effect of the virus .

福建省两株HTLV-Ⅰ前病毒外膜区部分env核苷酸序列测定及分析

Determination and Analysis of Partial evn Gene Nucleotide Sequences in Proviral Envelope Region of Two Strains of HTLV-I in Fujian Province

利用脂质体转染技术，将含有SNV株禽网状内皮组织增生症病毒（REV）前病毒全基因组cDNA克隆质粒转染鸡胚成纤维细胞（CEF）。

Chicken embryo fibroblast cells ( CEF ) were transfected with avian reticuloendotheliosis virus ( REV ) proviral cDNA clone mixed with lipofectamine .

方法用套式聚合酶链反应对23份甘肃省HIV-1感染者外周血淋巴细胞中前病毒脱氧核糖核酸（DNA）的膜蛋白基因进行扩增，并对C2-V3及其邻区300个核苷酸序列进行测定和分析。

Methods 23 DNA fragments of HIV-1 env gene were amplified by nested PCR from PBMC extracted from different subjects infected with HIV-1.The sequences of the env C_2-V_3 region were determined .

反转录病毒前病毒基因组长末端重复序列（LTR）对病毒复制有重要的调控作用，影响到病毒的复制动力学、细胞嗜性、致病力等方面。

The EIAV proviral genomic long-terminal repeat ( LTR ) is a eukaryotic promotor , which plays an important regulatory role on transcription and replication of the virus .

准确测定HIV1的前病毒载量和病毒载量的技术，在感染者预后和艾滋病患者药物治疗效果的评价以及艾滋病的其它研究方面，都具有十分重要的应用价值。

Accurate determination of HIV 1 proviral burden and viral load is very useful in prognosis of HIV 1 infected patients and in assessment of drug for therapy of AIDS patients .

目前正在寻找潜伏在猪体内危险的前病毒，并消灭之。

The hunt is on to find any potentially dangerous pig proviruses .

马传染性贫血病病毒驴白细胞弱毒疫苗株前病毒全基因组核苷酸序列分析

Complete Sequence of Proviral DNA of EIAV Chinese Donkey Leukocyte Attenuated Strain

调节转基因信号的另一个途径是运用可切割的前病毒产物。

Another pathway of transgenic signal-regulating was to apply sectorial product of provirus .