凝固酶

凝固酶阴性葡萄球菌及其ica操纵子检测的临床研究

Coagulase-negative Staphylococci and ica Operon Examination : A Clinical Study

其次为凝固酶阴性葡萄球菌（CNS），占21.7%；

Coagulase-negative S.aureus were 69 strains , 21.7 % of the total organisms ;

目的研究凝固酶阴性葡萄球菌（CNS）院内感染的危险因素。

Objective To explore the risk factors of coagulase negative staphylococcus induced nosocomial infection .

从30株血浆凝固酶阴性的葡萄球菌中分离13株耐甲氧西林血浆凝固酶阴性葡萄球菌（Methicilin－ResistantCoagulase－NegativeStaphylococciMR－CNS）。

13 Methicillin-Resistant Coagulase-Negative Staphylococci ( MR-CNS ) were identified from 30 Coagulase-Negative staphylococci .

革兰阳性菌中主要为凝固酶阴性葡萄球菌（CNS）、金黄色葡萄球菌、肠球菌，耐药性严重。

Gram positive bacteria mainly included Coagulase negativeStaphylococcus ( CNS ), Staphylococcus aureus and Enterococcus .

凝固酶阴性葡萄球菌（CNS）已经成为引起奶牛乳腺炎的常见病原菌，通常引起隐性乳腺炎或者表现温和的临床型乳腺炎。

Coagulase-negative staphylococci ( CNS ) have become the most common cause of bovine mastitis .

目的调查血培养中凝固酶阴性的葡萄球菌（CNS）血样本的污染率。

Objective To investigate the contamination incidence of coagulase - negative staphylococci ( CNS ) in blood culture .

目的分析凝固酶阴性葡萄球菌（CNS）所致血行感染的相关因素，为临床诊治CNS菌血症提供参考依据。

OBJECTIVE To analyze the related factors of blood stream infection caused by coagulase-negative staphylococci ( CNS ) .

目的：评价血培养中凝固酶阴性葡萄球菌（CNS）阳性的临床意义。

Objective : To evaluate the clinical significance of coagulase negative staphylococci ( CNS ) in blood culture .

目的探讨成都地区血浆凝固酶阴性葡萄球菌（CoNS）耐药性及分子流行病学特征。

Objective To investigate the antimicrobial resistance and molecular prevalence of coagulase-negative Staphylococcus ( CoNS ) in Chengdu .

在凝固酶阴性葡萄球菌中（CNS），44.0%（37／84）为结构型耐药，41.7%（35／84）为外排型耐药。

Active efflux mechanism was characterized in 35 / 84 ( 41 . 7 % ) strains of CNS .

目的：了解凝固酶阴性葡萄球菌（CNS）的感染情况及耐药现状。

Objective : To find out infection of coagulase-negative staphylococcus and their multiple drug resistance status and control nosocomial infections caused by CNS .

感染菌居前6位的分别是铜绿假单胞菌、酵母样菌、肺炎克雷伯菌、大肠埃希菌、金黄色葡萄球菌和凝固酶阴性葡萄球菌（CNS）。

The top six pathogens were Pseudomonas aeruginosa , yeast-like fungus , Klebsiella pneumoniae , Escherichia coli , Staphylococcus aureus and Coagulase Negative Staphylococcus .

目的了解玻片法凝固酶试验在GPI卡鉴定金黄色葡萄球菌（SA）中的影响。

[ Objective ] To study the influence of slide coagulase test on determining the staphylococcus aureus ( SA ) with GPI assay .

G+球菌中，以凝固酶阴性葡萄球菌（CNS）分离率最高，占G+球菌的57.0%（929/1629）。

The coagulase negative Staphylococci ( CNS ) accounted for 57.0 % ( 929 / 1,629 ), which was the most common among the G ~ + cocci .

试管法试验：EDTAK2抗凝人血浆与枸橼酸钠抗凝人血浆凝固酶阳性率均为97.06%；

In test tube method , the positive rate of coagulase tests was 97.06 % with both sodium citrate and EDTA-K 2 as plasma anticoagulant .

mecA基因阳性率在金黄色葡萄球菌和凝固酶阴性葡萄球菌中分别为32.3%和95.8%；

MecA detected in S.aureus and coagulase negative staphylococcus was 32.3 % and 95.8 % respectively .

目的：探讨耐甲氧西林凝固酶阴性葡萄球菌（MRCNS）在新生儿败血症中的分布、临床特征及耐药性特点。

Objective : To study the distribution and features of drug resistance of MRCNS in neonatal septicemia .

目的了解本院儿童血培养凝固酶阴性葡萄球菌（CNS）的感染率及其药物敏感情况，为儿科合理使用抗生素提供依据。

Objective To explore the infection and medicine susceptibility of blood culture coagulase negative staphylococcus ( CNS ) in children , and provide evidence for use of antibiotics in pediatrics .

目的研究血浆凝固酶阴性葡萄球菌（CNS）的生化反应特性，建立一种血浆凝固酶阴性葡萄球菌快速两步一补鉴定方法。

[ Objective ] To study biochemical characteristics of Coagulase-Negative Staphylococci ( CNS ) and develop a " two steps with one supplement " method for rapid identification of CNS species .

5年中耐甲氧西林凝固酶阴性葡萄球菌（MRCNS）分离率为76.6%；耐甲氧西林金黄色葡萄球菌（MRSA）分离率为1.9%；

The Methicillin-resistant coagulase-negative Staphylococci ( MRCNS ) rate were 76.6 % and Methicillin-resistant Staphylococcus aureus ( MRSA ) rate were 1.9 % .

病原菌中葡萄球菌8种37株，其中凝固酶阴性葡萄球菌（CNS）32株，耐甲氧西林葡萄球菌（MRS）22株。

Of all isolated pathogens , there were 37 strains of 8 species of staphylococcus . 32 strains of coagulase-negative staphylococcus ( CNS ) and 22 strains of methicillin resistant staphylococcus ( MRS ) .

最常见的病原菌是凝固酶阴性葡萄球菌（CNS），占15.7%，其次是金黄色葡萄球菌、大肠埃希菌、假单胞菌属、不动杆菌属。

Coagulase negative Staphylococcus ( CNS )( 15.7 % of the isolates ) was the most frequently isolated pathogen , followed by Staphylococcus aureus , Escherichia coli , Pseudomonas spp and Acinetobacter spp.

目的建立快速凝固酶阴性葡萄球（CNS）菌种的基因鉴定方法。

Objective To set up a rapid identification of Coagulase-negative Staphylococcus . Methods One hundred strains of Clinical isolates identified as members of the CNS by Auto Scan-4 and API Staph system .

目的研究淋菌性和非淋菌性尿道炎后慢性前列腺炎（CP）与凝固酶阴性葡萄球菌（CNS）的关系，探讨CNS耐药性检测对临床的指导意义。

Objective To study the relationship between chronic prostatitis following gonococcal and nongonococcal Urethritis and the coagulase negative staphylococci ( CNS ), and to explore the clinical significance of CNS drug resistance detect .

苯唑西林耐药金黄色葡萄球菌（MRSA）和苯唑西林耐药凝固酶阴性葡萄球菌（MRCNS）检出率分别为80.65%和87.73%；

The detectable rates of Methicillin-resistant S.aureus ( MRSA ) and Methicillin-resistant coagulase negative Staphylococci ( MRCNS ) were 80.65 % and 87.73 % ;

耐甲氧西林金黄色葡萄球菌（MRSA）检出率为39.1%，耐甲氧西林凝固酶阴性葡萄球菌（MRCNS）检出率为36.0%；

The detection rate of meticillin-resistant Staphylococcus aureus ( MRSA ) was39.1 % , and that of meticillin-resistant coagulase-negative Staphylococcus ( MRCNS ) was36.0 % .

结果MRS包括耐甲氧西林凝固酶阴性葡萄球菌（MRCNS）和耐甲氧西林金黄色葡萄球菌（MRSA）。4种标本中MRS总检出率为60.1%。

Results The detection rate of MRS ( including methicillin resistant coagulase negative staphylococcus ( MRCNS ), methicillin resistant staphylococcus aureus ( MRSA )) in all of the specimen was 60.1 % .

耐甲氧西林金黄色葡萄球菌（MRSA）和耐甲氧西林凝固酶阴性葡萄球菌（MRCNS）分别占金黄色葡萄球菌（金葡菌）和凝固酶阴性葡萄球菌的63%与77%，无万古霉素耐药株。

MRSA and MRCNS accounted for 63 % and 77 % of S aureus and coagulase negative staphylococci respectively , no vancomycin resistant strains were found .

方法：标本来自2001年7月~2003年10月大连医科大学附属第二医院收集的81株金黄色葡萄球菌，常规分离临床标本，经凝固酶试验、API系统鉴定为金黄色葡萄球菌。

Method : From july in 2001 to October in 2003,81 SA strains were obtained . All clinical isolates from the second affiliated hospital , Dalian medical university . All strains were identified by the Bacto coagulase plasma test and API system estimated .