冷冻保存

HBV转基因小鼠部分表型的分析及胚胎冷冻保存方法的研究

Analysis of Phenotype for HBV Transgenic Mice and Study on Cryopreservation of Embryos

基于SVM的鱼类胚胎冷冻保存试验设计

Experimental Design of Fish Embryo Cryopreservation Based on SVM

表明本试验中应用配方V冷冻保存精液，其解冻后的精子受精能力效果显著。

It shown ingredient V was the best of these ingredients on the ability of fertilization .

冷冻保存对骨髓单个核细胞IL-1和IL-6分泌的影响

Effects of Cryopreservation on IL-1 and IL-6 Secretion by Human Bone Marrow Mononuclear Cells

深低温冷冻保存的异体前交叉韧带移植修复重建ACL的临床研究

The Clinical Study of Restoring ACL by Allograft of Cryopreserved ACL

冷冻保存同种异体骨-前交叉韧带（ACL）-骨移植实验研究

An experiment on transplantation of cryopreserved bone anterior cruciate ligament ( ACL ) bone allograft

小鼠2-细胞胚胎细管法和OPS法玻璃化冷冻保存技术的研究

Study on Cryopreservation of Mouse 2-Cell Embryos by Straw or OPS Vitrification

结论富含血小板血浆冷冻保存后可获得较高回收率，血小板数量和血浆pH值没有明显改变。

Conclusions : We could acquire a high recovery rate of platelets from frozen PRP , and the BPC and pH value of PRP ware not significantly changed .

取肝右叶进行组织学检查，快速液氮冷冻保存相近部位肝组织待进一步行内皮素A受体mRNA原位杂交；

The second , histological changes of liver were estimated , endothelin receptor mRNA of liver was examed by in situ hybridization after keeping in liquid nitrogen .

目的在于为乙肝病毒表面抗原（HBsAg）转基因鼠胚胎和珍奇动物、濒危动物胚胎冷冻保存提供研究方法。

The work would provide an alternative method for cryopreservation of HBsAg-transgenic murine embryos and of rare and endangered animal embryos .

小鼠4-细胞胚胎OPS法冷冻保存技术

Vitrification of Mouse 4-Cell Embryos by OPS Method

方法取Wistar大鼠坐骨神经，经超低温冷冻保存后移植于SD大鼠坐骨神经缺损处。

Methods The sciatic nerve defect at SD rats was bridged by the sciatic nerve from wistar rats after ultra deep cryopreservation .

研究表明，GMP法为一种有效的猪卵母细胞冷冻保存方法。

The present study shows that GMP method is an efficient freezing method for porcine oocyte cryopreservation .

牙鲆（Paralichthysolivaceus）胚胎冷冻保存研究

Study on Cryopreservation of Flounder ( Paralichthys Olivaceus ) Embryos

本研究获得的主要结果如下：1、猪精液冷冻保存过程中，基础稀释液中添加生物类黄酮和维生素C均能有效提高猪精子冷冻-解冻后的质量。

The chief conclusions were as following . 1 . In the process of boar semen frozen conservation , both bioflavanoid and Vitamin Cadded into basic diluents could enhance quality of boar semen after freezing-thawing .

在精子冷冻保存液中加入二硫苏糖醇（DTT），观察复温后精子复苏率、运动速度、测定精子内ATP和精浆内GOT含量。

Dithiothreitol ( DTT ) could significantly improve the recovery and velocity of frozen-thawed spermatozoa when it was added in the protective medium .

由于AID的广泛应用及人类精子库的建立，关于冷冻保存对人精子遗传物质的影响日益受到重视。

Because of widespread application for AID and establish of human sperm storeroom , the effects of cryopreservation on human sperm genetic substance have been paid more and more attention .

结论SIS作为一种天然的细胞外基质材料，可显著提高胰岛细胞冷冻保存后的回收率，减少冻存胰岛细胞复苏时的死亡，并改善胰岛细胞的功能。

Conclusion Co-culture of cryopreserved rat islets with SIS can increase the recovery of islet cells and improve their function .

方法取Wistar大鼠股动脉，经超低温冷冻保存后行血管细胞培养及动脉移植；

Methods Femoral artery was harvested from Wistar rats . The cell culture of the vessel and artery transplantation were carried out after cryopreservation .

长期冷冻保存的外周血干细胞采集物形成CIK的能力及其生物学活性的研究

Study of the living ability and biologic activity of cytokine-induced killer cells from long-term cryopreservation PBSC collections

冷冻保存时4℃平衡1h、-80℃过夜，然后直接投入液氮中，解冻后培养得到61.6%的贴壁率。

The cryopreservation attachment rates was 61.6 % by 4 ℃ equating 1 h , overnight at-80 ℃, and then plunging into liquid nitrogen .

样品采集：动物分别于给药后2h和6h处死，迅速采集器官组织与体液检材，冷冻保存待检。

Sample collecting : Animals were executed 2h and 6h post dose , respectively . Tissues and body fluids were instantly collected .

结果表明，10%DMSO、10%PG及10%EG均适宜冷冻保存小鼠精原细胞，以10%DMSO的冻存效果最好，而G则不适宜冷冻保存。

The results suggest that 10 % DMSO , 10 % PG and 10 % EG are suitable for cryopreservation of mouse spermatogonia , and 10 % DMSO is the best , while G is unsuitable .

目的观察优选技术在精液冷冻保存中对常规精液参数、线粒体膜电位（△Ψm）和活性氧（ROS）的影响。

Objective : To investigate effects of cryopreservation and preparation on sperm parameters , mitochondrial membrane potential (△Ψ m ) and reactive oxygen species ( ROS ) .

粘附性淋巴因子活化的杀伤细胞抗自体白血病细胞作用的研究冷冻保存对肝癌患者粘附性LAK细胞生物学特性的影响

Study on the Cytotoxicity of Adherent LAK Cells against Autologous Leukemic Blasts Effects of Cryopreservation on Biological Character of Adherent LAK Cells from Patients with Hepatocellular Carcinoma

尤其是大鼠，因为其较长的精子尾巴，使得它的精子冷冻保存相当困难，并且大鼠的卵胞浆内精子注射（ICSI）技术也没有小鼠那样成熟。

Especially for rat , its long tail of sperm makes its sperm cryopreservation very hard . Accordingly , ICSI of rat is not done better than mouse .

目的：探讨新鲜异体和二步冷冻保存同种异体骨前交叉韧带（ACL）骨移植后排斥反应的差异。

Objective : To study the difference in rejection response between fresh anterior cruciate ligament ( ACL ) allograft and two step cryopreserved ACL allograft after transplantation in rabbits .

白细胞介素-2依赖细胞系CTLL的冷冻保存

Refrigeration Preservation of an Interleukin 2 ( IL_2 ) - dependent Cell Line CTLL

对比了不同方法冷冻保存外周血干细胞（PBSCs）的细胞活性、单个核细胞数、cFUGM和CD34+细胞回收率。

PBSCs were cryopreserved by different methods . Cell viability and recovery rate of mononuclear cells ( MNC ), CFU-GM and CD34 + cells were compared .

冷冻保存PGCs可以用于地方鸡种的保种，尤其是一些濒临灭绝的物种遗传资源的保存。

Refrigeration and preservation of PGCs can be used as strain protection of local chickens , in particular , some endangered genetic resources .