冰冻切片

结论：术中冰冻切片有助于确诊IP癌变。

Conclusion : Frozen section is helpful for definitive diagnosis .

对人工感染ALVJ鸡肾脏冰冻切片检测结果证明，免疫荧光法可以检测出组织中的ALVJ抗原，表现强阳性反应。

Frozen section of kideny infected with ALV_J showed positive but SPF chicken tissue negative in IFA .

方法：利用血凝块融化液直接进行DNA提取，并通过血凝块融化液涂片、血凝块冰冻切片以及琼脂糖电泳鉴定所提DNA的质和量。

Methods : DNA was extracted directly from the fluid at the bottom of semi-frozen blood clot .

方法常规方法制备SD大鼠脑组织冰冻切片。

Methods Frozen sections of SD rat brain tissues were prepared .

一氧化氮合酶，N末端，兔抗人，大鼠；冰冻切片，IH。

Nitric Oxide Synthase , N-terminal , Rabbit anti-Human , Rat ; frozen , IH .

用地高辛标记的cDNA探针，采取冰冻切片原位杂交的方法检测内皮素-1和BfgfmRNA的表达。

The expression of ET-1 and bFGF mRNA was evaluated by in situ hybridization with Dig labelled cDNA probe .

40μm厚冰冻切片，孵育后制作电镜标本，透射电镜下观察心肌细胞线粒体上细胞色素C氧化酶的活性变化。

The 40 μ m sections were incubated in Cox incubation solution , then prepared to TEM samples and the enzyme activity changes of Cox on mitochondria were observed by TEM .

方法：利用OCT包埋剂对152例不同种类囊壁样组织包埋进行冰冻切片。

Methods use OCT to freezing slice in the tissues of cystiform in 152 cases .

对含有毛发（hair）的动物药材进行石蜡切片、冰冻切片、毛小皮印痕特征的显微研究，记录图片与文字资料。

Microscopic identification of animal drugs possessed hair were conducted according to the characteristics of wax slices , frozen slices and hair cuticle moulage , and data were recorded .

选用MS培养基并附加不同浓度的外源激素MA、NAA和BA进行培养，定期做冰冻切片进行观查。

MS medium with IAA , NAA and BA in different concentrations for culturing and freezing slices at different times were observed .

用胰腺冰冻切片间接免疫荧光法成功地检测出血清中胰岛细胞抗体（ICA）。

Islet cell antibodies ( ICA ) were successfully detected with indirect immunofluorescence assay on frozen sections of human pancreas .

用免疫组织化学法，在冰冻切片上观察了不同胎龄组肝内前b细胞的形态、分布及其分化为b细胞过程中抗原的变化。

The morphology and distribution of pre-B cells and the changes of antigens during the differentiation from pre-B to B cells in fetal livers of different gestational ages were studied by means of immunohistochemical technique .

C组肾脏冰冻切片荧光强度及外周血ANA荧光滴度均低于A组和B组（P<0.05）。

The fluorescence intensities of mice kidney frozen sections and ANA titres in group C were lower than that in group A and B ( P < 0.05 ) .

黑色素聚集激素（MCH），鸡抗人，小鼠，大鼠；冰冻切片，IH。

Melanin Concentrating Hormone ( MCH ), Chicken anti-Human , Mouse , Rat ; frozen , IH .

运用放射免疫化学原理，灌流固定，冰冻切片，定影，显影，观察ACE在正常大鼠及模型大鼠肾脏中的分布。

Using RIA chemical theory , Perfusion fixed , Frozen section , fixation , imaging , observation ACE in normal and model mice kidney .

方法经过灌流固定的CNS组织低温冰箱速冻后连续冰冻切片和改良的苏木精染色方法。

Methods After perfused fixation and quick freezing , the tissues of whole CNS were cut into serial sections with cryostat followed by hematoxylin stain .

误诊病例中，术前CT检查8例、细针穿刺细胞学检查3例，术中快速冰冻切片检查5例。

Cases ( 8 / 26 ) received CT scanning , 3 cases ( 3 / 26 ) were performed FNAB detection before operation and quick frozen pathologic section ( 5 / 26 ) in operation was low .

casPase一3蛋白免疫组化检测冰冻切片经正常山羊血清工作液，室温孵育lh（不洗）；

Immunohistochemical detection of caspase - 3.The sections were incubated by using 10 % normal goat serum in PBS for 1 hour at room temperature ;

结论运用低温速冻、连续冰冻切片、改良的苏木精染色方法可制作同时显示CNS神经元和神经纤维的全程连续切片。

Conclusion Our method was feasible to show both neurons and nerve fibers at the same section with quick freezing , freezing serial sectioning and improved hematoxylin staining .

冰冻切片与细胞爬片或涂片用含0.1%TritonX-100和0.1%枸橼酸三钠混合液作穿透处理。

Frozen tissue sections , adherent cells and smears were permeated with 0.1 % Triton X-100 in 0.1 % sodium citrate .

方法制备成年大鼠脊髓的冰冻切片，用免疫组织化学染色方法研究NGB蛋白在成年大鼠脊髓内的分布及细胞定位。

The localization of NGB protein in the spinal cord of adult rat was examined by immunohistochemistry method .

目的：评价幼虫冰冻切片抗原间接荧光抗体试验（IFAT）诊断旋毛虫病的价值。

AIM : To evaluate the value of indirect fluorescent antibody test ( IFAT ) in diagnosing trichinosis .

用成虫冰冻切片作IFA可出现3种不同的免疫荧光反应类型。

Four hybridoma-derived antibodies were measured by IFA with frozen sections of adult worms and 3 different patterns of immunofluorescence reaction were clearly revealed .

方法：用成年猫L3脊髓节段制作20μm厚的冰冻切片并分两组。

Firstly , the L3 segments of spinal cords of adult cats were taken and made into frozen sections ( 20 ?μ m ) .

目的应用血管造影和组织冰冻切片荧光显微镜观察的方法，研究国产新型光敏剂血啉甲醚（hematoporphyrinmonomethylether，HMME）在兔脉络膜黑色素瘤眼部组织的分布。

Objective To investigate the distribution of hematoporphyrin monomethyl ether ( HMME ) in rabbit choroidal melanomas by dye fluorescence angiography and fluorescence microscopy .

取出WGA－HRP注射区，脊髓颈段和延髓，切制连续冠状冰冻切片（厚40μm）。

The cervical spinal cord , medulla oblongata and WGA-HRP injecting area were cut into coronal sections at 40 μ m thickness on a freezing microtome .

分别于术后1周、2周、1个月和2个月时取脑、冰冻切片，进行Nissl染色和BrdU免疫荧光检测。

After grafting for 1 week , 2 weeks , 1 month and 2 months , BrdU-labeled cells were detected by Nissl staining and BrdU immunofluorescence .

对同窝三种不同基因型小鼠的耳蜗冰冻切片应用免疫组织化学方法染色观察，阴性对照为野生型小鼠耳蜗的冰冻切片并用PBS代替一抗，阳性对照为小鼠肾脏组织冰冻切片。

As a negative control , frozen sections of the cochlea were obtained from wild-type mice with no antibodies and a positive control , frozen sections of mouse kidney were used .

应用冰冻切片、酶解分离、荧光显微技术和傅里叶红外光谱分析FTIR等手段，对华山松初生根和针叶内皮层凯氏带进行了分离、显微结构特征和化学成分的比较。

Casparian strips were isolated from primary roots and needles by enzymatic isolation , the cellular structure was observed under the fluorescence microscope and chemical characterization was analyzed by Fourier transform infrared ( FTIR ) spectroscopy .

MNNG诱发大鼠胃癌前期肝中碱性磷酸酶、酸性磷酸酶同工酶变化的冰冻切片观察

Ice sections watching on the changs of ALP isoenzyme and ACP isoenzyme in liver at the early stage of gastric cancer induced by MNNG