免疫电镜法

方法离心超滤和蔗糖密度梯度离心法分离K562细胞释放的exosomes，固相免疫电镜法（SPIEM）制备exosomes的HSP70、ICAM-1及ABL免疫电镜标本。

Methods Exosomes were purified by ultrafiltration centrifugation and sucrose gradient ultracentrifugation . The expression of HSP70 、 ICAM-1 、 ABL were investigated by solid phase immunoelectron microscopic method ( SPIEM ) .

用免疫电镜法对大鼠孤束核内侧亚核内NT-LI神经元的超微结构进行了观察。

Using electron immunocytochemical method the ultrastructural distribution of neurotensin-containing neurons in the medial subnucleus of the nucleus of solitary tract of the rat was studied .

若在标本中加入荧光标记的抗体，可提高检出率，称为免疫电镜法。

Adding fluorescent labeled antibodies to samples can improve detection rate , which is known as immune electron microscopy .

本文详述了用免疫电镜法鉴定苜蓿病毒分离物M&4及不同时期感染该病毒的不同寄主叶片细胞超微结构的研究。

The paper detal the identification of the virus isolated M-4 from alfalfa by IEMM and study on ultrastructure of leaf cell of different host which infected the virus in different time .

当病毒滴度低时，免疫电镜法仍有较高的假阴性，且由于价格昂贵，技术条件要求高，不适合大规模的流行病学调查。

However , when the virus titer is low , there is still a high false negative , and it is expensive , demanding better technical conditions , so it is not suitable for large-scale epidemiological investigations . 2 .

为了探讨神经递质在癫痫发病机理中的作用，用包埋前免疫电镜双标法研究了正常大鼠海马CA1，区谷氨酸（Glu）神经元与GABA神经元之间的突触联系。

In order to explore the roles of different neurotransmitters in epileptic pathogenesis , the synaptic connections between glutamic acid neurons and GABA neurons in normal rat hippocampus were studied by pre-embedding double labeling immunoelectron microscopy , i. e.

本文用免疫电镜负染法对病毒性乙型肝炎患者13份粪便进行观察，检出了HBsAg，与实验室检查结果相符。

By immune electron microscopic observation on 13 fecal specimens ( negative stain ) from patients with hepatitis B virus , HBsAg was found in some of the specimens .

具有标记靶作用的固相免疫电镜快速诊断法（SPA&GIEM）

A method of rapid diagnosis with marking action for solid phase immune electron microscopy ( spa-g iem )

采用3H渗入检测法、免疫电镜、ELISA法、雏鸭感染试验等技术，观察短波紫外线（UVC）与交联淀粉碘（CSI）联用对血浆中鸭乙型肝炎病毒（DHBV）的灭活效果。

Using techniques such as 3H permeation examination method , immunological electronmicroscopy , ELISA and duckling infection test , the efficacy of combination of short-wave ultraviolet rays ( UVC ) and cross-linked starch iodine ( CSI ) in inactivating DHBV in plasma was observed .

本实验应用包埋前免疫组织化学电镜法，进一步研究了这种神经纤维的超微结构，及其与腺细胞的关系。

In the present study , the ultrastructure of substance P-like immunoreactive nerve fibers and their relationship with the gland cells of the pars distalis in the dog were investigated by use of pre-embedding immuno-electron microscopy .

应用免疫电镜和负染法检测马铃薯试管苗中的微量病毒。

Immune electron-microscope and negative stained methods were used to examine the trace virus in tube plants of potato .

目前，TGE和PED的诊断方法主要有病毒中和试验、免疫荧光法、免疫电镜法、ELISA和RT-PCR法等。

At present , main diagnostic methods to TGE and PED are virus neutralization ( VN ), immunofluorescence ( IF ), immune electron microscopy ( IEM ), ELISA and RT-PCR .