保守序列

CUE结构域是一个大约含有40个氨基酸的保守序列，，它不但能够识别细胞内的单泛素还能识别多泛素。

CUE domain is a small conserved sequence containing approximately 40 amino acids , which can recognize both mono-ubiquitin and poly-ubiquitin .

花生MADS-box基因保守序列的克隆与分析

Cloning and Sequencing of Peanut MADS-box Gene Conserved Sequence

以硼转运蛋白基因的保守序列作为探针进行Southern杂交分析，结果显示有7条杂交条带。

Southern blotting analysis indicated that 7 hybridization bands could be detected with a conserved sequence of boron transporter genes as a probe .

利用一对兼并引物，通过PCR分别从不同来源芽孢杆菌基因组DNA上扩增出β-甘露聚糖酶编码基因的一段保守序列。

Using a pair of degenerated primers , the conserved fragments of β - mannanase gene from the selected strains were amplified by PCR .

Toll样受体（Tolllikereceptors，TLRs）是果蝇Toll的同系物，它们广泛存在于植物及动物体内，是物种进化保留下来的保守序列。

The mammalian Toll-like receptors ( TLRs ) are homologues of Drosophila Toll and conserved sequence during species evolution .

根据植酸酶基因的保守序列设计引物P1、P2，从云芝中分离克隆植酸酶基因的片段。

P1 , P2 were designed according to the consensus sequence of phytase gene .

根据其它植物中ACC氧化酶基因的保守序列，设计合成一对简并引物；

The double degenerate primers were designed by the homologous sequence of the ACC oxidase genes .

不同-10区保守序列的tac启动子表达睾丸酮丛毛单胞菌中的活化因子

Expression of Activator from Comamonas testosteroni with tac Promoter Containing Different-10 Consensus Sequences

甘薯块根总RNA提取与psy基因保守序列的克隆

Isolation of total RNA from tubers of Ipomoea batatas and cloning of conserving domain sequence of psy gene

根据α-珠蛋白基因起始密码子上游及终止密码子下游的保守序列设计引物，以牦牛基因组DNA为模板克隆并测定了α-珠蛋白基因序列。

The α - globin gene of yak was amplified and cloned by using the primers based on the conserved region of the upstream from the initiation codon and the downstream from the termination codon .

同源性比较显示，不同物种来源的CyclinE周期蛋白盒具有较高的保守性，有35个氨基酸为共同保守序列。

Homology comparison revealed that the cyclin box regions of cyclin E proteins from different species were highly conserved , among which 35 amino acids were identical .

一级结构分析发现无同源序列，但可分为6个家族（family），其中5个家族各自具有保守序列，家族6无保守序列。

They have no homologous sequences by analysis of the primary structure , but can be divided into six families . Five families of them have their respective same series , and family six has no the same series .

利用双子叶植物花发育基因的保守序列筛选水稻的基因组文库或cDNA文库已发现70多个同源异型基因。

Now over 70 homeotic genes were isolated from rice by using the conserved sequences of the homeotic genes of dicots as probe screening the rice genomic library or cDNA .

以免疫球蛋白信号肽序列为引物进行半套式PCR所得到的产物在质和量上都优越于以可变区5′末端保守序列为引物进行PCR所得到的产物。

The products by half nested PCR using signal peptide sequences as primers were superior in quality and quantity to those by PCR with conserved sequences in the 5 ′ end variable regions as primers .

盐藻Psy基因保守序列的克隆及同源性分析

Cloning and Homology Analysis of the Conservative Sequence of Phytoene Synthase Gene from Dunaliella Salina

根据GenBank中已登记的番茄、马铃薯等蔗糖磷酸合成酶基因的保守序列设计特异引物，采用RT-PCR方法，从番茄自交系20-19果实总RNA中克隆到目的基因的全长cDNA。

PCR primers were designed based on the consensus domain of some sucrose phosphate synthase genes registered in GenBank . A aimed complete cDNA was obtained by using RT-PCR from tomato fruit .

所有脂肪酶一级结构中都包含GlyX1SerX2Gly保守序列，在真菌脂肪酶中该序列更保守；

The consensus sequence in all lipase primary structures is Gly X 1 Ser X 2 Gly , which is more conserved in fungi .

根据蔷薇科树种S基因的保守序列设计引物，利用PCR技术对8个新疆扁桃品种S基因进行扩增，获得了10个大小不同的S基因片段。

To investigate the characteristics of the S gene in almond , we degenerative primers according to conservative sequence of S gene in rosaceous plant and got 10 S gene fragments by PCR application from 8 almond cultivars in China .

首先通过该转运系统ATP结合蛋白氨基酸的保守序列设计简并引物一对，获得约560bp的部分核苷酸序列。

Depending on the conserved amino acids of glycine betaine ABC transporter , a pair of degenerate primers were designed .

结果筛选得到6个能与人AChE较强结合的噬菌体克隆，其中有4个克隆可以抑制AChE的酶活性，其保守序列为W（S/P）HY。

Results Six positive phage clones binding to human AChE were obtained , and 4 of them sharing the conservative sequence W ( S / P ) HY inhibited the enzyme activity of AChE .

根据不同植物肌动蛋白基因编码区的保守序列设计引物后进行RT-PCR，并采用RACE技术扩增出actin基因cDNA全长序列。

The degenerate primers were designed according to the conserved sequence of gene encoding region in different plant actin , RT-PCR was carried out , and the full length cDNA of birch actin gene was amplified by RACE .

RGD是许多粘附蛋白结构中的高度保守序列，与细胞在生物材料表面的粘附、增殖密切相关。

Many kinds of adhesive proteins contain Arg Gly Asp ( RGD ) sequence , which is closely related to cell adhesion and proliferation on biomaterial surfaces .

根据GenBank中已经发表的IBVN基因的保守序列，设计合成一对引物，利用RT-PCR技术扩增IBVN基因的582bp的核酸片段，并制备出地高辛标记的IBV核酸探针。

According to the genomic sequences of N gene of infectious bronchitis virus ( IBV ) published in Genbank , a pair of primers was designed for amplifying the 582 bp fragment in RT-PCR experiments .

本试验对GenBank中报道的猪附红细胞体的基因序列进行同源性比较分析，选择猪附红细胞体的保守序列作为扩增区域。

The experiment analyzed the homology of gene sequence of Eperythrozoon suis what were reported in GenBank , to select its conserved sequence as amplification district . The homology between C.

该2序列均有多个与转录调控有关的保守序列（如TATA-box、CAAT-box）和富含GT的重复序列等许多相似之处。

There were several conserved promoter motifs such as TATA-box , CAAT-box , and the tandem GT sequence in the sequences .

根据抗病基因NBS-LRR保守序列不同区域设计4个简并引物，形成不同组合。

The main results are as follows : Four oligonucleotide primers were designed based on the conserved NBS-LRR domain of R genes .

比较2种阅读框架，以保守序列作为模板扩增得到完整的编码EIAV反式激活蛋白的基因。

The whole sequence of tat gene has been obtained by further polymerase chain reaction during which more conservative sequence was used as template .

通过与其他物种氨基酸序列比对发现，这个GST属于Pi型，具有谷胱甘肽结合位点（G位点）和外源底物结合位点（H位点）的高度保守序列。

The comparison of the deduced amino acid sequences with GSTs from other species showed that the enzymes belonged to the pi-class and the amino acids defining the binding sites of glutathione ( G-site ) and for xenobiotic substrates ( H-site ) were highly conserved .

本试验根据IBRV的gE基因保守序列，设计并合成了一对特异性引物，应用该对引物分别建立了常规PCR及荧光定量PCR检测方法，并对模拟临床样品进行了检测。

This experiment according to the IBRV gE gene conservative sequence designed and synthesized a specificity primer , application of the primer , we established PCR and Real-time fluorescent quantitative methods , and use these methods to testing simulation samples .

2利用PrimerPremier5.0、oligo6.0等软件根据已报道的苹果、梨等果树和模式植物拟南芥相关基因的高度保守序列设计了简并引物。

According to the reportes about highly conserved sequence of apple trees , pear trees or the other woody years plants and the model plant Arabidopsis thaliana , the proper primers were designed by primer premier 5.0 and oligo 6.0 software .