亮蓝

考马斯亮蓝法检测蛋白质含量；

Protein content was measure with Coomassie light blue .

考马斯亮蓝G动力学光度法测定痕量铁的研究

Determination of Trace Iron by Catalytic Spectrophotometry with Coomassie Brilliant Blue G

测定亚硝酸根的新指示反应研究-氯酸钾-考马斯亮蓝G体系

Study on a Indicating Reaction for the Determination of Nitrite With Potassium Chlorate-Coomassie Brilliant Blue G System

钛Ⅳ催化考马斯亮蓝G褪色光度法测定痕量钛

Kinetic spectrophotometric determination of trace titanium based on the catalytic effect of titanium ⅳ on the discoloring of coomassie brilliant blue G

超声破碎表达的细菌，通过SDSpage及考马斯亮蓝染色，检测融合蛋白AβHBcAg的表达。

The expression of the fusion protein A β - HBcAg was detected by SDS-PAGE .

用SDS-PAGE（考马斯亮蓝染色）检测融合基因的表达。

The expressed fusion protein was analyzed by SDS-PAGE .

DNS法和考马斯亮蓝法检测提取品无还原糖和蛋白质。

Neither reducing sugar nor protein was determined by DNS and Bradford method respectively .

检测指标：考马斯亮蓝法检测24h尿蛋白含量；

Detection of indexes : The 24-hour urine protein was detected with Coomassie brilliant blue method ;

SDS-PAGE检测目的蛋白纯度和相对分子质量；考马斯亮蓝法测定超滤液中蛋白浓度。

Its molecular mass and concentration were determined by SDS-PAGE analysis and Coomassie blue staining method separately .

方法制备艾蒿花粉和豚草花粉的花粉浸液，采用SDS-PAGE及考马斯亮蓝染色分析两种花粉粗浸液中的蛋白质组分；

Methods Extract of artemisia pollen and ragweed pollen were prepared SDS-PAGE and Coomassis were used to analyze proteins .

实验证明，在相同的条件下，漆酶粗酶对RB亮蓝有更好的脱色效果。

It was also indicated that the crude enzyme of laccase was more efficient under the same decoloring conditions .

经SDS-PAGE电泳、考马斯亮蓝染色及脱色后，结果表明酰胺酶基因成功的得到了表达。

Through SDS-PAGE electrophoresis , coomassie brilliant blue staining and decoloration , the results showed that amidase gene was successfully expressed .

改良EDTA法提取巴氏杆菌内可溶性抗原成分，经透析浓缩后保存，并取用考马斯亮蓝法测定其浓度。

The soluble antigens of Pasteurella were extracted by improved EDTA method and were preserved after having been dialysed and concentrated .

以亮蓝染料作为模型化合物，对不同亮蓝染料浓度、静置吸附时间、金属担载量、溶液pH值等条件下金属/硅藻土的吸附性能进行了研究。

Was used as a model compound . The effects of Brilliant blue dye concentration , time , metal loading and pH value of the wastewater on adsorptive behavior of metal / diatomite were investigated .

方法采用细胞培养、MTT比色测定、碱性磷酸酶测定法、考马斯亮蓝法及茜素红染色法。

Methods Using cell culture technique , MTT colorimetric assay , ALP activity assay , Commasie brilliant blue staining and Dahl McGec Russell 's alizarin red stain for calcium .

考马斯亮蓝G250光度法测定水样中阳离子表面活性剂

Spectrophotometric Determination of Cationic Surfactants in Water with Coomassie Brilliant Blue G250

盐酸吡格列酮的考马斯亮蓝G250光度测定法

Spectrophotometric method for determination of pioglitazone hydrochloride with Coomassie brilliant blue G250

PAGE是蛋白质分析、比较和特性鉴定的有效方法，常规SDS-PAGE分析蛋白过程中考马斯亮蓝色素脱除过程耗时低效。

PAGE is an effective method for the analysis , comparison and identification of protein . The removal of the coomassie brilliant blue ( CBB ) from the gel is generally inefficient in SDS_PAGE .

蛋白质电泳考马斯亮蓝G250染色方法改良

An Improved Technique for Protein Staining with CBB G250

方法：将SDSpage中的蛋白分别用银染、铜染和考马斯亮蓝染色，并对铜染或考马斯亮蓝染色后的蛋白进行相应的复染。

Methods : The proteins in SDS PAGE were stained with coomassie brilliant blue staining , copper staining and silver staining . Then the proteins were restained after coomassie brilliant blue staining or copper staining .

考马司亮蓝G-250染色法测定羊毛酶减量

Measuring weight loss of the wool treated by enzyme with Coomassie Brilliant Blue

纯化的GAD在变性条件下电泳，经考马斯亮蓝R250染色以及westernblot鉴定主要有两条带，分子量分别为67kD和44kD。

The purified GAD showed two main bands , 67 kD and 44 kD , on SDS-PAGE by Coomassie Brilliant Blue R-250 and Western-Blot under reducing condition .

考马斯亮蓝G-250染色法测定草乌中可溶性蛋白质含量

Determination of Protein Contents of Radix Aconiti Kusnezoffii Using Coomassie Brillant Blue G-250 Dye Binding

分别采用ICP-MS法和苯酚硫酸法及考马斯亮蓝法对硒多糖和硒蛋白中硒、多糖及蛋白含量进行测定；

The contents of selenium , polysaccharide and protein in Se-polysaccharide and Se-protein were determined by ICP-MS , phenol-sulfuric acid method and Coomassie brilliant blue method .

采用考马斯亮蓝G250检测17种变应原的蛋白质含量，与重量/体积法对照，发现按后者配制的变应原皮试液蛋白质含量悬殊。

Coomassie Brilliant Blue method was adopted to detect the protein content of seventeen kinds of allergens .

用考马斯亮蓝G250法测定获得的CEPO蛋白浓度。

The protein concentration of CEPO was determined by the method of Coomassie brilliant blue G250 .

通过细胞形态观察、油红O染色、香草醛测总脂法和考马斯亮蓝测总蛋白等方法分析不同浓度AA和DHA对大鼠前体脂肪细胞分化的影响。

By observed cells morphologic changes , oil red o staining lipids assay , vanilline total fat and total protein assay method to detect regulation of AA and DHA on adipocytes differentiation .

结果：以适当浓度的考马斯亮蓝R-250的磺基水杨酸水基染色液染色，能保证聚丙烯酰胺凝胶和PVDF膜在染色前后保持等长。

Results : The gel and PVDF membrane stained by staining solution of suitable concentration can keep same length with unstained counterpart .

westernblot分析：将样品加适量样品悬浮缓冲液后，超声破碎，离心取上清，考马斯亮蓝法测定蛋白浓度。用10%SDS-PAGE电泳后，将PAGE凝胶中的蛋白转移至硝酸纤维素膜。

Western blot analysis : Add appropriate amount of sample suspension buffer to sample , sonication , the centrifugal supernatant , Coomassie-blue method for the determination of protein concentration . 10 % SDS-PAGE electrophoresis , the PAGE gel , proteins were transferred to nitrocellulose membrane .

蛋白质测定方法主要有考马斯亮蓝法（CBBG-250）、紫外吸收法（UV）和凯氏定氮法。

There are mainly three methods in the determination of proteins : Coomassie brilliant blue G-250 ( CBB G-250 ), UV absorption ( UV ) and micro-Kjeldahl .