互补决定区
hù bǔ jué dìnɡ qū
- 名complementary determinant region
互补决定区
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方法:利用RT-PCR扩增9例APL患者和10例正常人外周血单个核细胞中29个TCRVα亚家族基因的互补决定区3(CDR3),了解TCRVα亚家族T细胞的分布;
Methods : The complementary determining region 3 ( CDR3 ) of TCR V α 29 subfamily genes in peripheral blood mononuclear cells from 9 APL patients were amplified using RT-PCR .
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利用RT-PCR扩增12例B-CLL外周血单个核细胞的TCRVβ基因24个亚家族的互补决定区3(CDR3),以了解T细胞的表达情况。
To observe the expression of TCR V β subfamily T cells , complementarity determining region 3 ( CDR3 ) of TCR β with 24 variable region genes was amplified in peripheral blood mononuclear cells from 12 B-CLL cases using RT-PCR .