biocytin

Methods Biocytin intracellular staining method was used in present study .

方法应用biocytin细胞内染色方法。

The technique of biocytin staining combined with patch-clamp whole cell recording of visual cortex

与视皮层脑片膜片钳全细胞记录相结合的生物素标记技术

We successfully labeled 11 CD cells and 10 CID cells with biocytin .

用生物胞素作追踪剂我们成功标记了11个CD型和10个CID型细胞。

Retinal ganglion cells were stained by Lucifer Yellow or biocytin which were injected into cells during whole cell recordings for the purpose of morphological study of RGCs .

全细胞记录的同时细胞内注入荧光黄或biocytin进行组织染色，观察RGCs的形态学特征。

The sections with intact structure were hatched for 1 hour , and were recorded with blind patch clamp , Biocytin staining was used to confirm the recorded cells .

取结构较完整的切片室温下孵育约1h，盲法膜片钳行全细胞记录。记录的细胞经biocytin染色，确定细胞类型。

Pre-synaptic test stimulation was given at 0.07 Hz intervals through bipolar stimulating electrodes . EPSCs of layer ⅱ ~ ⅳ were recorded by recording electrodes filled with 0.5 % biocytin .

通过视皮层的双极刺激电极给予0.07Hz的突触前测试刺激，记录视皮层神经元的PSCs。