BACS

Rapid Modification of BACs With The Optimized Red / ET Recombination System

Red/ET同源重组介导细菌人工染色体的快速修饰

Third , modified audit opinion has some negative correlations with BACs .

非标审计意见与恶意会计政策变更有一定的负相关关系。

The system used to deposit salaries in bank accounts , BACs , is on yet another network .

用于向银行账户储蓄薪水的系统bacs使用的又是另一个网络。

Laws that establish BACs of0.05g/dl or below are effective at reducing the number of alcohol-related crashes .

将血液酒精浓度设定为0.05克/每分升或者更低的法律，对于减少与酒精相关的撞车数量具有效果。

The library was screened by using 20 microsatellite markers and 5 known functional genes with PCR . Positive BACs for all these markers and genes were identified successfully and the positive number ranges from 3 to 19 with average of 11.3 .

用20个微卫星标记和5个功能基因对整个文库进行PCR筛选，所有这些标记和基因都得到了阳性克隆，克隆数从3到19不等，得到的平均阳性克隆数为11.3。

Average insert size of the library is 118 kb evaluated from analysis of 452 BACs randomly picked from library . So the genome coverage of the library is 13.34 and the possibility to find a single-copy gene in the library is 99.984 % .

对随机挑选的452个BAC克隆的插入片段进行鉴定估计文库的平均插入为118kb，文库的基因组覆盖率为13.34倍，预计从文库中筛选到单拷贝基因的机率为99.984%。