骨髓细胞培养

方法用不同浓度的mCSF和活化核因子κB的受体配体（RANKL）与鼠骨髓细胞培养，在加入或不加入牛骨片的情况下诱导产生破骨细胞。

Methods The bone marrow cells of mice were treated with different concentration of M 、 CSF and RANKL in vitro . The mice stromal cells were cultured and osteoclast was supported with or without bone slice .

目的研究不同骨髓细胞培养方法对大鼠破骨细胞样细胞（OLC）诱导分化及活性的影响。

Objective To compare the differentiation and function of osteoclast-like cells ( OLC ) derived from bone marrow cells by different cultural methods .

骨髓细胞培养均发现集落减少，集簇增加。

The culture of medullary cells showed that colonies decreased and clusters increased .

着重做了血流变、血动力、微循环及骨髓细胞培养。

Method : Hemorheology , hemodynamics , microcirculation and bone marrow cell culture were studied .

观察照射动物的一般临床表现、外周血细胞计数、骨髓细胞培养。

The general condition , blood cell counts and bone marrow cell CFC assays were observed .

长期骨髓细胞培养净化白血病细胞与临床疗效关系的研究

Purging of leukemic cells from long-term culture of bone marrow cells and its relation with therapeutic efficacy

观察了小鼠脾脏、胸腺和骨髓细胞培养上清中的粒系造血刺激活性和抑制活性及其在照射后的改变。

The granulopoietic stimulating and inhibitory activities in the conditioned media of mouse spleen , thymus and bone marrow cells were estimated and the changes of these activities after irradiation were investigated .

~（60）Co照射小鼠骨髓基质细胞培养的形态和功能观察

Observation of morphology and function of mouse marrow stromal cells

白血病患者骨髓细胞CFU-GM培养及药敏试验分析

CFU-GM Culture and Analysis of Drug Sensitivity of Bone Marrow Cell of Leukemic Patients

利用ABC法对胎儿骨髓细胞长期培养贴壁细胞层的6种凝集素受体的分布及动态变化进行定性分析。

The localizations and changes of6 lectin receptors of the adherent layer cell in long-term bone marrow cultures of human fetus were studied by ABC method .

结论：自体骨髓细胞体外培养对CML病人的骨髓肿瘤细胞有一定程度的净化作用；

Conclusions : Bone marrow culture in vitro may be useful to purge bone marrow autografts in some patients with CML .

目的：探讨慢性髓性白血病（CML）骨髓细胞体外培养对自体骨髓移植物的净化作用。

Objective : To evaluate the purging effect of bone marrow culture in vitro on the autografts of patients with chronic myelogenous leukemia ( CML ) .

应用骨髓细胞体外培养技术，观察了国产重组人白细胞介素3（rhIL－3）对人骨髓粒单祖细胞集落的影响。

Using hematopoietic progenitor culture , effect of rhIL 3 on human bone marrow progenitor cell colony formation was assayed .

方法：用骨髓细胞短期培养法和直接法G显带分析染色体；双色荧光原位杂交检测bcr/abl融合基因。

Method : Chromosome karyotype of bone marrow cells was studied by short time culture and direct method of Giemsa banding metaphase , the status of bcr / abl fusion gene was studied by dual-color fluorescence in situ hybridization ( D-FISH ) technique .

采用半固定培养骨髓细胞，培养基中分别加有白细胞介素5（1ng/ml）和不同浓度的地塞米松（0~0.2mg/ml），计数嗜酸性粒祖细胞克隆（EoCFU）。

Bone marrow Eo-CFU were enumerated using a semisolid culture assay with 1ng / ml interleukin-5 and different concentration of dexamethasone ( from 0 to 0.2mg / ml ) .

细胞融合成片时间，骨髓细胞为培养10～14d，脐带血细胞为培养12～18d；

Cells confluence was detected at 10 ~ 14 days in bone marrow culture and 12 ~ 18 days in cord blood culture .

结果：RT-PCR检测确定0.1mg/mL的HLA-G1表达于转染的K562细胞上，后与正常骨髓细胞共同培养。

Results : RT-PCR was used to detect the expression of HLA-G1 ( 0.1mg / mL ) on the K562 cell , and the transfected K562 cell was co-cultured with normal bone marrow cells .

采用骨髓基质细胞培养法观察经YS2H作用，培养4周时的正常人及白血病患者骨髓基质细胞形态变化。

The morphology of healthy persons and leukemia patients of bone marrow stromal cells with bone marrow stromal cells cultivation by YS2H were studied after 4 weeks .

用Ⅱ型登革病毒（D2V）与人骨髓细胞混合培养48小时后，测定上清液中干扰素的浓度，发现D2V感染组的干扰素浓度明显高于空白对照组（P<0.001）。

The concentration of interferon detected in the supernatant fluid of Dengue-2 virus ( D2V ) in human bone marrow cell culture cultured 48 hours was significantly higher than the control group ( P < 0.001 ) .

生三七人参皂甙对小鼠骨髓细胞体外培养的作用

Proliferative effects of raw ginsenoside on murine bone marrow cell in vitro

大鼠骨髓基质细胞培养方法的改良

Improvement of the culturing method of rat bone marrow stromal cells in vitro

小鼠骨髓细胞体外培养中贴壁细胞层的结构与作用

Study on structure and function of adherent cell layer In mouse bone marrow long term culture

豚鼠胸腺与骨髓细胞混合培养体外造血的研究

Hemopoiesis in vitro of mixed culture of bone marrow cells and thymus cells in guinea pigs

骨髓基质细胞培养基对体外脊髓运动神经元的影响

The influence of culture fluid of bone marrow stromal cells on spinal cord motor neuron in vitro

大鼠损伤脊髓提取液对骨髓基质细胞培养特性的影响

Effects of the traumatized spinal cord extracts on the proliferation and differentiation of rat bone marrow stromal cells in vitro

本文报道了云产三七根粗提物&生三七人参皂甙对小鼠骨髓细胞体外培养有促进细胞团增殖的作用。

This paper reports that raw ginsenoside , extract from panax notoginseng from Yunnan province , could promote the proliferation of routine bone marrow cells in vitro .

成年SD鼠骨髓基质干细胞培养及向神经元样细胞定向分化的研究

Culture and Differentiation of Mesenchymal Stem Cells From Adult SD Rat Into Neuron-Like Cells

方法骨髓细胞半固体培养和悬浮培养，DNA凝胶电泳，DNA缺口末端标记（TdT），电镜观察。

Methods Semisolid culture , DNA agarose electrophoresis , in situ terminal deoxynucleotidyl transferase assays , view with electromicroscope .

细胞因子IL-4对小鼠骨髓来源树突状细胞培养的影响

Effects of cytokines IL-4 on dendritic cell precursor from mice bone marrow in vitro

同时将骨髓白血病细胞体外培养72h，进行药物杀伤效应实验，分别比较MTZ、柔红霉素（DNR）在体外对ALL细胞不同分化阶段的抑制作用。

The killing effects of MTZ were investigated by the culture of the leukemic cells in vitro .