荧光指示剂

本论文以钌（Ⅱ）&联吡啶（Ru（bpy）3Cl2）为荧光指示剂，醋酸纤维素（celluloseacetatemembrane，简称CA膜）为荧光指示剂固定的基质材料，制备出光纤氧传感膜。

In this thesis , Ru ( bpy ) 3Cl2 is used as fluorescent indicator and cellulose acetate ( CA ) as the matrix to form the fiber optic oxygen sensing membrane .

通过对FIA法测定液体石油产品族组成分析中所用的原材料如硅胶、吸附柱、荧光指示剂等的质量进行分析，指出了其对分析结果的影响；

The quality of such raw material as silica gel , absorption column , fluorescent indicator when testing liquid petroleum products group with FIA is analyzed and the its effect on the analysis result is indicated .

方法：用荧光指示剂Fura-2/AM结合荧光显微镜成像系统检测单个PM基态的及用激动剂刺激细胞后的[Ca2+]i；

METHODS : I implicated stimulated were measured by fluorescent microscopic imaging system after loading with fluorescent probe fura-2 / AM .

Fura-2荧光指示剂法研究稀土离子跨红细胞膜的作用

Study on the Transmembrane Behavior of Rare Earth Ions in Erythrocytes by Fura-2

方法用钙离子荧光指示剂Fluo-3/AM负载小鼠腹腔巨噬细胞，以激光共焦镜显微镜观察不同浓度的DNA对巨噬细胞内游离Ca2+离子的浓度变化。

Methods The macrophages were loaded with Fluo-3 / AM . The free calcium ion concentration of macrophages was detected by employing laser scanning confocal microscopy , after the macrophages were stimulated with different dosage of DNA .

运用钙离子荧光指示剂Fura－2／AM，检测了粉防己碱（Tet）对成年大鼠心室肌细胞电压依赖性钙通道的影响。

The effect of tetrandrine on voltage-dependent calcium channel of ventricular myocyte was investigated by fluorescein Fura-2 / AM in rats .

采用MTT法和测微网及钙荧光指示剂Fura2／Am测定培养的肺动脉平滑肌细胞生长情况和收缩功能以及细胞内游离Ca2＋浓度。

Cellular growth , constrictions function and free Ca 2 + in cultured pulmonary artery smooth muscle cells were measured by the methods of MCT , morphometry and calcium fluorescent probe Fura 2-Am .

AngⅡ+卡维地洛组：卡维地洛1μmol/L与AngⅡ0.1μmol/L同时加入。以Fluo3/AM荧光指示剂负载，应用激光共聚焦显微镜技术，分别于加入干预药物后即刻与15min检测[Ca2+]i变化。

Confocal microscope was used with Fluo-3 / AM as calcium indicator to detect changes of [ Ca 2 + ] I immediately and 15 minutes after drug intervention respectively .

方法：将血管紧张素Ⅱ（AngⅡ）作用于肾小球系膜细胞，加入不同浓度的积雪草提取物，采用MTT法和Fura-2/AM荧光指示剂负载测定MC增殖和细胞内[Ca2+]I的变化。

Methods : Rat MC were incubated with angiotensin ⅱ( Ang ⅱ), To observe the proliferation and intracellular free calcium concentration by the MTT method and flurescence measurement with Fura-2 / AM after given the extracts from ASA .

利用钙荧光指示剂Fura2/AM，观察Bre对Thro、ADP引起的血小板内游离钙（[Ca2+]i）的影响。

Using fluorescence indicator_Fura 2 / AM , to determine the effects of Bre on intra platelet free calcium elevation induced by Thro and ADP .

方法：采用离体豚鼠心Langendorff法灌注，胶原酶I型分离细胞，再用荧光指示剂方法（Fure-2/AM）标记心肌[Ca2+]i变化。

Methods : We adopted guinea pig heart Langendorff instillation . The myocardial cells were isolated by collagenase ( Tyoe I , sigma ) and marked by fluorescence ratio imaging .

本实验采用分离的SD大鼠心室肌细胞，以Fura－2AM荧光指示剂负载，检测心肌细胞内游离钙浓度（〔Ca2＋〕i）变化。

The mechanism underlying Leu-enkephalin ( LEK ) induced increase of the intracellular concentration of free calcium ( [ Ca2 + ] I ) in rat ventricular myocytes was investigated by using fura-2 AM as a calcium indicator .

方法选用新生Wistar大鼠原代培养海马神经元，建立谷氨酸细胞毒性模型，借助于共聚焦显微镜结合钙荧光指示剂Fluo-3-AM观察OA对海马神经元[Ca2+]i的影响。

Methods Glutamate cytotoxic model of primary cultured neurons from neonatal Wistar rat hippocampal was used to evaluate the effect of OA on the change of cytosolic calcium in cultured hippocampal neurons by means of confocal microscopy with Fluo-3-AM .

方法：应用Ca2+荧光指示剂Fura-2/AM分别测定了10周龄、22周龄、34周龄SHR心肌细胞内Ca2+浓度以及导管法测定了大鼠心功能，并以同龄京都-Wistar（WKY）大鼠作对照。

METHODS : Intracellular free calcium concentrations were measured by Fura 2 methodology and left ventricular function quantitated by cardiac catheterization in 20 SHR aged 10 , 22 , and 34 weeks and 20 age-matched Wistar-kyoto ( WKY ) rats .

在此基础上，应用Ca2+荧光指示剂技术和NADH氧化偶联差光谱变化的分析方法，检测了前述泡沫样细胞的胞浆Ca2+水平及膜成分Ca2+-ATP酶活性。

With the technique of Ca 2 + fluorescent indicator and the assay of NADH-oxidizing coupling spectrum-alteration , the intracellular Ca 2 + level and membranous Ca 2 + - ATPase activity of the above foam-like cell were determined .

方法：用MTT法检测细胞增殖，用流式细胞术检测膜IL-2受体（mIL-2R）和细胞增殖周期，用荧光指示剂Fura-2/AM检测细胞内[Ca2+]，将IMS后的结果与正常组进行比较。

Methods : The proliferation of lymphocytes was examined by MTT , and intracellular [ Ca2 + ] was examined by fluorescence probe , Fura-2 / AM , membrane of lymphocytes IL-2 receptor ( mIL-2R ) and cell cycle were examined by flow cytometry .

方法：以Fura2/AM为细胞内钙离子的荧光指示剂，用双波长荧光分光光度计分别测定不同条件下的BEL7402细胞内[Ca2+]i。

Methods : Intracellular free calcium concentration ( [ Ca 2 + ] I ) in human hepatoma BEL-7402 cells of different groups was measured by double wavelength spectrofluorometer with Fura-2 / AM as the fluorescence indicator .

方法：用Pulsineli四动脉结扎法造成大鼠急性前脑缺血40min后再灌注1h的损伤模型，同时应用Fura－2／AM荧光指示剂测定硫酸锌对胎鼠脑细胞内游离钙浓度的影响。

METHODS : Animal model was produced by occlusion of four vessels . At the same time , free intracellular calcium concentration was measured by using calcium sensitive fluorescent indicator Fura 2 / AM in isolated embryonic rat brain cells .

ESWL后24h获取肾脏，采用Fura-2/AM荧光指示剂测定肾细胞内[Ca2+]i浓度，原位缺口末端标记法（TUNEL）和流式细胞计数法检测凋亡细胞。

The kidneys from rabbits were obtained 24 hours after ESWL . Renal intracellular [ Ca2 + ] I level was measured by Fura-2 / AM fluorescence assay , cell apoptosis was detected by TUNEL ( TdT-mediated dUTP Nick End Labelling ) and flow cytometry at the same time .

方法：采用钙离子荧光指示剂Fura-2/AM和双波长检测技术测定细胞内游离钙的浓度。

Methods : Calcium fluorescent indicator fura-2 / AM was used .

荧光指示剂法测定植物细胞胞质游离Ca~（2+）浓度

Measurement of cytoplasmic free calcium in plant cells using fluorescence indicator

荧光指示剂在细胞内金属离子测定中的应用

Application of fluorescent indicators in measurements of metal ions in cells

荧光指示剂检测铅的细胞毒性作用

Fluorescence indicator as a probe for detecting cytotoxicity of lead

荧光指示剂柱色谱吸附法测定汽油组成影响因素的研究

Studies on Influence Factors of Method of Fluorescent Indicator Adsorption

基于荧光指示剂共价固定的新型光化学传感器的研制

Novel Fluorescence Sensors Based on Covalent Immobilization of Fluorescent Carrier

该化合物有望成为一种新型的Ca~2+的荧光指示剂。

This compound was expected to be a new kind calcium fluorescent indicator .

液体石油产品烃类测定法-荧光指示剂吸附法

Liquid petroleum products-determination of hydrocarbon types-fluorescent indicator adsorption method

应用荧光指示剂奎宁与单体苯乙烯交联共聚制备二氧化硫荧光传感探头，研究其响应机理并应用于二氧化硫的检测。

Sulfur dioxide fiber was developed through the copolymerization of fluorescence indicator quinine and monomer styrene .

用Fura－2／AM为荧光指示剂测定〔Ca2＋〕i。

[ Ca 2 + ] I was measured with Fura 2 / AM as fluorescent indicator .

荧光指示剂吸附分析

Fluorescent indicator adsorption analysis