荧光共振能量转移

吖啶橙-罗丹明B荧光共振能量转移猝灭法测定砷

Fluorescence Resonance Energy Transfer Quenching Method for Determination of Arsenic with Acridine Orange-Rhodamine B

CdTe量子点与罗丹明B间的荧光共振能量转移研究

Fluorescence Resonance Energy Transfer between CdTe Quantum Dots and Rhodamine B

CdTe与Au之间的荧光共振能量转移研究

Fluorescence Resonance Energy Transfer between CdTe and Au

庆大霉素与水溶性CdTe量子点的荧光共振能量转移作用

Fluorescence Resonance Energy Transfer between Gentamycin and Water-Soluble CdTe QDs

基于荧光共振能量转移技术，结合量子点和金纳米颗粒优良的光谱特性，发展了一种用于人IgG检测的新型免疫分析方法。

Based on the FRET between functionalized QDs and Au nanoparticles , a novel method was developed to detect human IgG .

目的：建立以荧光共振能量转移（FRET）电压敏感探针为染料的钾通道抑制剂高通量筛选模型。

AIM : To establish high-throughput potassium channel assay using FRET-based voltage sensor probes ( VSPs ) .

此外，在这一章里还介绍了本论文中使用的主要实验手段：停流（stopped-flow）技术和荧光共振能量转移（Forsterresonanceenergytransfer，FRET）技术。

In addition , we introduced the experimental methods used in these studies : stopped-flow technique and Forster resonance energy transfer ( FRET ) technique .

PKCε与Lck在细胞内直接相互作用的荧光共振能量转移证据

Fluorescence Resonance Energy Transfer Evidence of Direct Interaction Between PKC ε and Lck in vivo

TPM染料的荧光共振能量转移光谱和荧光共振能量转移滴定可把G-四链体（包括分子内G-四链体和分子间G-四链体）与单链和双链DNA区分开。

The energy transfer fluorescence spectra and energy transfer fluorescence titration can be used to distinguish G-quadruplexes ( including intramolecular and intermolecular G-quadruplexes ) from single-and double-stranded DNAs .

目的构建可在体外应用荧光共振能量转移（FRET）技术检测具有酶活性的前列腺特异抗原（PSA）的生物检测器。

AIM To construct a fluorescence resonance energy transfer ( FRET ) - based biosensor for detecting the enzymatically active prostate-specific antigen ( PSA ) .

具体方法包括原位的邻位连接技术（insituPLA），荧光共振能量转移技术（FRET）技术以及免疫金电镜技术。

These methods include in situ proximity ligation assay ( in situ PLA ), fluorescence resonance energy transfer ( FRET ) as well as immunogold with transmission electron microscopy .

他还开发、改进了荧光共振能量转移（FRET）技术，以监测两种被荧光标记的分子之间的相互作用。

Tsien has also exploited and improved fluorescence resonance energy transfer ( FRET ) for detecting interactions between two fluorescently labeled molecules ( 5,6 ) .

对这种复合物结构认识的依据来自于荧光共振能量转移技术（FRET），该技术能够测量两个分子之间的距离。

The evidence for this complex structure came from special technique called FRET ( Fluorescence Resonance Energy Transfer ) that can measure the distance between two molecules .

实时定量聚合酶链反应（real-timeQuantitativePCR，RQ-PCR）是综合PCR、分子杂交和酶动力学的一种新技术，它根据荧光共振能量转移（fluorescenceresonanceenergytranslation，FRET）的原理进行。

Real-time quantitative polymerase chain reaction ( RQ-PCR ) is a new technology , which develops from PCR ? hybridization and enzymic technology , and it is base on the principle of the fluorescence resonance energy translation .

而荧光共振能量转移（FRET）技术近年来被广泛用于信号通路的研究，具有直接灵敏的特性。

However , fluorescence resonance energy transfer ( FRET ) technology been widely used in the signaling pathway in recent years , and has a direct and sensitive features .

荧光共振能量转移（FRET）在荧光染料分子的设计中起着举足轻重的作用，已成为有机化学在各个领域应用的不可或缺的重要桥梁。

Fluorescence resonance energy transfer ( FRET ) plays an important part in the design of fluorescent dyes and has become an essential bridge connecting organic chemistry and other fields .

荧光共振能量转移（FRET）是指能量供体和受体之间由于远程偶极-偶极相互作用，在激发状态下能量从供体荧光团向受体荧光团转移的非辐射过程。

Fluorescence resonance energy transfer ( FRET ) is a nonradiative process whereby an excited state energy donor transfers energy to a proximal ground state energy acceptor through long-range dipole-dipole interactions .

首先，优化了细胞培养、磷酸钙转染方法，使用458nm激光代替430nm激光来进行荧光共振能量转移（FRET）探测。

Firstly , we optimize the protocols of hippocampal primary culture and calcium phosphate transfection ; and try to replace 430 nm laser with 458 nm laser for monitoring the FRET ratio of Cameleon .

目的利用荧光共振能量转移（FRET）技术在活体细胞研究人Toll样受体（TLR）4与髓样细胞分化蛋白2（MD-2）的相互作用。

Objective To study the interaction between Toll-like receptor ( TLR ) 4 and myeloid differentiation protein-2 ( MD-2 ) in living cells using fluorescence resonance energy transfer ( FRET ) technology .

所得胶束不仅具有光致变色性能而且还能够通过荧光共振能量转移（FRET）来调控NBD的荧光，在活细胞中也能观察到此现象。

The obtained micelles not only have photochromic properties , but also modulate the fluorescence of NBD through fluorescence resonance energy transfer ( FRET ), which was also observed in living cells .

FRET技术及其在蛋白质-蛋白质分子相互作用研究中的应用应用荧光共振能量转移技术研究Dok分子的异源及同源相互作用

Fluorescence Resonance Energy Transfer Assay : Applications in The Study of Protein-protein Interaction Detection of the Homo - or Hetero-interaction of Doks with Fluorescence Resonance Energy Transfer in Living Cells

本文运用分子克隆、细胞GABA摄取检测、免疫细胞化学、免疫共沉淀和荧光共振能量转移测定等方法，研究了SNARE复合体对GAT-1的调控机制。

Here we investigated the mechanism for the regulation of GAT-1 by the SNARE complex using molecular cloning methods , GABA uptake detection , immunocytochemistry , coimmunoprecipitation and fluorescence resonance energy transfer ( FRET ) measurements and so on .

方法利用荧光共振能量转移的原理，设计检测人葡萄糖6磷酸脱氢酶（G6PD）基因的荧光杂交探针，在熔解曲线模式下，设定不同模板量、Mg2+浓度，观察熔解曲线峰面积及其Tm值。

Methods A pair of G6PD specific fluorescein labeled oligonucleotide hybridization probe were designed according to the principles of fluorescence resonance energy transfer . The melting curve peak area and melting temperature were observed at different concentrations of template and Mg 2 + for the mode of melting curves .

微腔中单分子对荧光共振能量转移光谱学的理论研究

Theoretical Study of Single-Pair Fluorescence Resonant Energy Transfer Spectroscopy in Microcavity

荧光共振能量转移体系的研究与应用

The Studies and Applications of Fluorescence Resonance Energy Transfer System

荧光共振能量转移效率的实时定量测量

Real-time quantitative fluorescence resonance energy transfer measurements using fluorescence microscopy

用荧光共振能量转移技术构建的蛋白酶检测器

Construction of a Novel Protease Biosensor Based on Fluorescence Resonance Transfer Technique

荧光共振能量转移测定牛血清白蛋白的研究

Study on determination of bovine serum albumin by fluorescence resonance energy transfer

连接酶介导的诱导荧光共振能量转移法检测基因点突变

Point Mutation Detection Using Ligase-mediated Induced Fluorescence Resonance Energy Transfer

荧光共振能量转移显微术及其新进展

Fluorescence resonance energy transfer microscopy and its recent development