细胞抽提物

无细胞蛋白质合成系统是一种以外源mRNA或DNA为模板，通过在细胞抽提物的酶系中补充底物和能源物质来合成蛋白质的体外系统。

The cell free protein synthesis system enables the direct in vitro synthesis of protein from template DNA or mRNA by using the existing enzymes in the cell extract , which is supplemented with substrates and energy sources .

玉米细胞抽提物在寡核苷酸指导的基因修复中的作用

Effect of Maize Cell - free Extract on Genetic Repair Directed by Oligonucleotides

报道了含SV40复制起点的质粒DNA在真核细胞抽提物中进行复制的DNA体外复制系统的建立。

In vitro DNA replication system derived from eukaryotes is capable of replicating exogenous plasmid DNA containing SV40 origin of replication efficiently and accurately .

在体外去除HeLa细胞抽提物中的Elongator复合物可降低其转录染色质模板的速率，也为人Elongator复合物参与转录延伸提供了生化证据。

In vitro , the removal of Elongator complex from the HeLa nuclear extracts can decrease the rate of transcription of chromatin templates , which also provides biochemical evidence that human Elongator complex takes part in the transcription elongation .

方法：以TRF1抗体应用免疫共沉淀方法，从细胞蛋白抽提物中分离TRF1蛋白复合物，并作蛋白质肽指纹谱鉴定；

Methods : The co immunoprecipitation assay was employed to isolate TRF1 protein complex and the immunoprecipitate was subjected to MALDI TOF mass spectrometry for protein identification .

免疫印迹分析发现，以1∶3000稀释的该多克隆抗体为一抗时，能够在转染了全长PAPC质粒的HEK293T细胞的蛋白质抽提物中，特异地识别出150ku的印迹条带。

In dilution of 1 ∶ 3 000 the antibody recognized a specific band in Western blotting analysis of FL-PAPC transfected HEK 293T cells lysates , which could be specifically blocked by pre-adsorption of prokaryotic expressed GST-PAPC fusion protein .