牛膝

牛膝醇提物就是在此理论指导下完成的，已在临床应用多年，取得较好的临床效果。

The root of bidentate achyranthes formulating is completed under being that here theory guides , already in applying many years clinically , get fairly good clinical effect .

牛膝精对血细胞的效应

The Effects of Extract of Radix Achyranthis Bidentatae on Blood Cells

目的：以怀牛膝的子叶为外植体，以愈伤指数与齐墩果酸含量优选最佳MS培养基。

Objective : To select the best MS medium .

川牛膝HPLC指纹图谱构建与药材质量评价研究

Study on HPLC Fingerprints Construction and Medicinal Materials Quality Evaluation of Cyathula Officinalis Kuan

对川牛膝及其伪品进行了IR光谱及UV导数光谱的测定。

The author has made IR and UV spectroscopic assay of cyathula and its counterfeit .

应用RAPD标记分析了19个川牛膝种群的遗传多样性。

Applied Mark RAPD analyzed 19 genetic diversity of Cyathula Officinalis .

牛膝多糖对脑外伤大鼠脑组织中SOD与MDA影响的实验研究

The effect of Achyranthis polysaccharides on SOD and MDA in the rats'brain tissue with cerebral trauma

方法利用特异性PCR技术对牛膝的rDNAITS区碱基序列进行测定，报道了牛膝的rDNAITS区的碱基序列。

Bidentata . Methods To determine rDNA ITS base sequence by peculiar PCR technology .

牛膝的rDNAITS序列分析

Ribosomal rDNA ITS sequence analysis of root of Achyranthes bidentata

目的：探讨牛膝精（abe）对血细胞的效应。

Objective : to demaonstrate the effects of Abe on different blood cells .

水溶性CdTe量子点对牛膝种子萌发的影响

Effects of Water Soluble CdTe QDs on Seed Germination of Achyranthes Bidentata

提示怀牛膝皂甙A的子宫兴奋作用可能与5-HT及PG的合成与释放有关。

It is suggested that the excitant action on uterine of ABSA has some thing to do with PG and5-HT .

结论：（1）应用牛膝多糖能初步纠正肺癌和哮喘患者Th1和Th2细胞因子的失平衡。

CONCLUSIONS : ( 1 ) ABPS can rectify the unequilibrium of Th1 / Th2 cytokines in PBMC from patient with asthma or lung cancer .

再用不同浓度的怀牛膝有效成分对抗去甲肾上腺素（NE）引起的血管收缩，观察其舒张程度。

Secondly , the norepinephrine ( NE ) induced vasoconstriction was antagonized by different concentration of active components of Achyranthes bidentata Blume , and its diastolic level was observed .

发现怀牛膝多糖ABP能延长小鼠凝血时间（CT）、大鼠血浆凝血酶原时间（PT）、白陶土部分凝血活酶时间（KPTT）。

We found ABP prolonged coagulation time ( CT ) in mice , increased plasma prothrombin time ( PT ) and kaolin partial thromboplastin time ( KPTT ) in rats .

结果表明，不同取代度的羟烷基化牛膝多糖对Lewis肺癌和B16黑色素瘤均有明显的抑制作用，同时能明显促进Lewis肺癌小鼠的NK细胞活性。

The bioactive results suggest that hydroxyalkylated AbPS were able to inhibit the growth of these two kind of tumors , and improve the activity of NK cell of mice bearing Lewis lung cancer .

并通过样品甲基化和GCMS分析，对羧甲基牛膝多糖糖链中羧甲基的分布进行了研究，结果表明羧甲基牛膝多糖中羧甲基主要取代在糖链中呋喃果糖的4位上。

By the methylation method and GC MS analysis , we studied the distribution of carboxymethyl groups in the sugar backbone of CM AbPS and conclude that the carboxymethyl groups mainly substitute on 4 position of the fructofuranose of the sugar backbone .

Mankin评分结果：模型组、牛膝总皂甙组Mankin评分显著高于正常组（P0.05）。

The results of Mankin score : The scores of model group and bidentata saponins group were significantly higher than the normal group ( P0.05 ) .

牛膝引药作用对双氯芬酸钠抗佐剂型关节炎大鼠的影响中药外用配合TDP治疗膝骨关节病63例

Therapeutic Effect of Radix Achyranthis Bidentatae Guiding Function on Diclofenac Sodium Induced Adjuvant Rats Arthritis External Application of Herbal Medicine and TDP in Treatment of 63 Cases of Knee osteoarthritis

目的：研究牛膝根多糖（ABPS）在体外对单核细胞活性的调节及诱导单核细胞HLA-DRα表面分子的表达。

OBJECTIVE To study the activated effects of Achyranthes bidentata polysaccharides ( ABPS ) on human monocytes in vitro , inducing HLA-DR_ (α) surface molecules expression of monocytes .

提取工艺：淫羊藿、骨碎补和牛膝采用醇提取有效成分，用L9（34）正交实验进行优选最佳提取工艺。

Extraction process : Drynaria , Epimedium , Achyranthes by alcohol extraction of active ingredients , with the L9 ( 34 ) orthogonal experiment to select the best extraction .

方法：建立脑外伤大鼠模型，分别在外伤后不同时相观察正常组，模型组，牛膝多糖高、中、低剂量治疗组大鼠脑组织中ROS，XOD和MPO的变化。

Methods : After making the cerebral trauma model , we observe the change of ROS and XOD and MPO in the rats ' brain tissue at different time in each group .

本文采用正交设计对怀牛膝总皂苷提取方法进行了研究，选择乙醇浓度、溶剂量、提取时间以及次数作为影响ABS醇提取工艺的4个主要因素。每个因素3个水平进行试验。

The optimal extraction process was selected with the orthogonal design , taking four main factors which effected the selection process : ethanol concentration , solvent volume , extraction time , extraction of the frequency of three levels of each factor to test .

结果，以上三种方法的相对标准偏差（RSD）均小于5%，加标回收率范围在88.23%～102.98%之间，不同品种牛膝生药材的相应元素含量之间多数存在显著或极显著差异。

The results showed that relative standard deviations of the above-mentioned three methods were all less than 5 % and the recoveries were 88.23 % ~ 102.98 % . Most of the contents of different elements were significantly different among different drugs .

通过对液体培养条件的选择，发现牛膝毛状根在MS液体培养基上（25℃、110r/min暗培养）获得最大生长速率，经25d培养，牛膝毛状根鲜重增加了8.4倍。

The highest growth rate of hairy roots was observed on MS liquid medium ( shaked at 110r / min at 25 ℃ in darkness ) , and their fresh weight was increased by 8.4 times after culturing for 25 days .

方法：牛膝关节25只，分别制作关节软骨损伤模型，扫描序列采用SPIR-3D-FFE-T1WI扫描序列。

Methods : Models of cartilage injuries were established in25 cattle knees , with SPIR-3D-FFE-T1WI sequence in the detection of knee injuries .

方法采用MTT细胞增殖实验观察中药补骨脂、川牛膝的两种活性成分：异补骨脂素和蜕皮甾酮对ERα、ERβ阳性细胞T47D增殖的影响；

Methods The influence of isopsoralen and ecdysterone ( the active principles of Fructus Psoraleae , Radix Cyathulae ) on the proliferation of ER α and ER β cells and on the expression levels of ER α and ER β mRNA by MTT assay and RT-PCR respectively .

结果：发现牛膝多糖浓度为0.8mg/ml时，对细菌的细胞粘附抑制最为明显，粘附率由（257.0±5.2）个细菌/细胞降低到（63.6±3.6）个细菌/细胞。

Results : Hela cell adherence assay data showed that the ABPS can obviously inhibit the adherence at the concentration of 0 8mg / ml , the adherence rate was reduced from 257 0 ± 5 2 organisms / cell to 63 6 ± 3 6 organisms / cell .

牛膝水提取物化学成分的研究

Studies on the chemical constituents of water extract from Achyranthes bidentata

结论：牛膝散能显著降低大鼠全血粘度。

CONCLUSION : Niuxi Powder can markedly reduce rats'whole blood viscosity .