海马伞

【方法】建立双侧穹隆海马伞切断的AD大鼠模型；

Methods AD models were made by transecting bilateral fimbria - fornix ;

将前3组大鼠切断穹窿-海马伞制作AD动物模型。

Fimbria-fornix ( FF ) of the first three groups was transected to make AD models .

穹窿海马伞损伤模拟Alzheimer's病模型大鼠脑MRI研究

Study of brain MRI signal changes of rat model of Alzheimer 's disease by transected unilateral fimbria-fornix

方法：应用穹窿海马伞切断AD大鼠模型，分别用同位素方法和蛋白质印迹检测ERK的水平。

Methods : Rat model of AD was made by transection of rat fimbria-fornix .

在脑立体定位仪上切断动物的穹隆-海马伞，并同时切除双侧卵巢，建立模拟AD动物模型。

And fimbria-fornix of brain was transected and ovary was cut off in the stereotaxic apparatus in order to make AD model .

穹隆海马伞切断大鼠海马PKC和ERK活性的变化

The investigation of PKC and ERK of hippocampus in transection of rat fimbria - fornix

穹窿海马伞损伤对成年鼠学习记忆和海马GFAP阳性细胞的影响

Effect of Fimbria-Fornix Injury on Learning and Memory and GFAP Positive Neurons of Hippocampus in Rats

NOV蛋白在切割海马伞大鼠海马中表达的变化

The change of Nov protein expression in the fimbria transected hippocampus of rats

结论穹窿海马伞切割侧海马提取液可明显促进神经干细胞分化为神经元和AChE阳性神经元。

Conclusion Extracts of hippocampus in fimbria fornix transection side may obviously promote NSCs differentiating into the neurons and AChE positive neurons .

选用雄性SD大鼠35只，在单侧穹隆海马伞损伤后一周，将胚胎基底前脑神经元植入损伤侧海马。

35 male SD rats received left fimbria fornix transection one week before transplantation . The fetal basal forebrain neurons were injected into the hippocampus of injured rats .

结论：穹窿海马伞切断AD大鼠海马ERK升高，说明ERK信号转导途径在AD发病早期有改变。

Conclusion : The level of ERK increased in hippocampus of rat model of AD , which showed changes of signal transduction route in the early phase .

方法：取健康雌性Wistar大鼠25只，体重220～250g，随机分为5组：①穹隆-海马伞切断加双侧卵巢切除组；

Method Healthy female wistar rats were randomly divided into 5 groups : ( 1 ) control group ;

穹隆海马伞切断与去势大鼠脑内nAChR与NGF表达

The Study of Expression of nAchR and NGF in the Brain of Ovariectomized Rats with Transection of Fimbria / fornix

方法切割SD大鼠右侧穹窿海马伞，14d后取两侧海马制成提取液；

Methods The extracts from fimbria fornix transected and intact hippocampi were respectively obtained at 14 day after transecting right fimbria fornix of adult SD rats .

目的：探讨穹窿海马伞（FF）损伤鼠学习记忆能力与海马胶质纤维性蛋白（GFAP）阳性细胞之间的关系。

Ve : To investigate the relationship between the GFAP-positive neurons of hippocampus and the learning and memory in rats with Fimbria Fornix ( FF ) injury .

穹隆海马伞切断与卵巢切除大鼠皮质区及Meynert核区雌激素受体α的表达

Expression of estrogen receptor alpha in cortex and Meynert nucleus of rats with fimbria / fornix transection and ovariectomy

方法：成年SD大鼠，切断一侧穹窿海马伞（FF），术后分别存活1、2、3、4周，进行NADPH－d组织化学方法染色。

Methods : The left sides of hippocampi fimbria-fornix in adult rats were transected , 1,2,3 , and 4 weeks after the operation , NADPH-d histochemistry staining method was used to detect NOS-positive neurons .

目的了解碱性成纤维细胞生长因子（bFGF）对海马伞切断造成的阿尔茨海默病（AD）模型大鼠海马齿状回神经发生的影响，探讨bFGF治疗AD的前景。

Aim To study the effect of basic fibroblast growth factor ( bFGF ) on neurogenesis in dentate gyrus of rat model of AD and to explore the application prospect of bFGF in treatment of AD with unilateral fimbria fornix transection .

方法：在立体定位仪上横切SD大鼠大脑皮质、胼胝体和穹隆海马伞，于术后1～30d处死动物。

Methods : The cortex , corpus callosum and fimbria-fornix of adult SD rats in the lesion group were cut transversely under a stereotaxic apparatus . The rats were sacrificed at 1-30 d post-lesion .

结果与对照组大鼠脑内nAchR表达比较，穹隆-海马伞切断组大鼠脑内海马CA1区、皮层区nAchR表达显著减少（P<0.05）；

Results In fimbria / fornix transection group , the expression of nAchR obviously decreased in the observed areas of the brain ( P < 0.05 ) .

取切割SD大鼠海马伞后14d及正常海马组织的匀浆进行非变性聚丙烯酰胺凝胶电泳（Native-PAGE）。

The hippocampi of SD rats on the 14th day after fimbria transection and the normal ones were extracted for Native-PAGE . ( 2 ) fimbria / fornix transection and ovariectomized group ;

38只Wistar大鼠双侧穹窿－海马伞损伤后.以被动回避反应为指标观察到记忆明显受损，与损伤前相比，有记忆动物自65.3%降至13.6%，两者差别非常显著。

Memory impairments of passive avoidance response were observed in 38 Wistar rats with bilateral fornix-fimbria transection . After fornix-fimbria lesions the degree of performance decreased from 65 . 3 % to 13 . 6 % ( P < 0 . 01 ) .

提示切割穹窿海马伞后海马中PEBPmRNA和蛋白的表达增高可能与海马中自体或植入的神经干细胞向神经元或胆碱能神经元的分化有关。

The results indicated that the higher expression of PEBP mRNA and protein in the fimbria-fornix transection side hippocampus may involve the hippocampal neural regeneration which induce the neural stem cells differentiating into neurons or cholinergic neurons .

结合本课题组以往的工作，本研究结果提示，切割海马伞后海马中高表达的NOV蛋白可能参与了诱导神经干细胞迁移和向神经元分化的过程。

The present results combined with our previous studies suggest that the over expressed NOV protein in the fimbria transected hippocampus may be concerned with the process of inducing neural stem cells migrating and differentiating into neurons .

目的：观察穹隆海马伞切断与卵巢切除大鼠脑内皮质区、基底前脑Meynert核区雌激素受体α表达的变化，探讨胆碱能和雌激素系统与认知障碍相关的发病过程。

AIM : To discuss expression of estrogen receptor - α ( ER - α) in cerebral cortex and Meynert nucleus in brain of rats with fimbria / fornix transection and ovariectomy , and study the pathologic process between the cholinergic system and the estrogen system .

穹窿海马伞损伤诱导神经前体细胞增殖和迁移

Proliferation and migration of neural precursor cells induced by fimbria-fornix injury

双侧海马伞切断阿尔茨海默病模型大鼠存在感觉门控能力的不足。

AD model rats whose bilateral fimbria-fornix is transected have deficiency of SG .

结论切断穹窿海马伞可引起动物学习记忆障碍。

Conclusion Fimbria fornix transection lesion can results in rats learning and memory disorder .

穹窿海马伞切割大鼠海马内Brn-4mRNA的表达变化

The change of Brn-4 mRNA expression in rat hippocampus after fimbria / fornix transection

双侧卵巢切除加穹隆-海马伞切断组（OF组）；

Fornix-hippocampal fimbria transected and ovariectomized group ;