氢化酶

产甲烷菌的MV氢化酶活性分析法

The Analytical Method of Activity of Methane-producing Bacteria 's Hydrogenase

氢化酶可以分为NiFe氢化酶、Fe氢化酶及无金属氢化酶。

There are three classes of hydrogenase : ( NiFe ) hydrogenase , ( Fe ) hydrogenase and metal-free hydrogenase .

结论-克隆了阴道毛滴虫氢化酶体腺苷酸激酶基因，序列测定及同源性分析表明，所测基因与Genbank中阴道毛滴虫氢化酶体AK序列具有高度同源性。

Conclusion The gene of AK has high homology with the AK gene sequence in Genbank .

测序表明，所克隆的AK基因大小为690bp，编码229个氨基酸。序列分析表明，所测基因与Genbank中阴道毛滴虫氢化酶体AK序列具有高度同源性（999%）。

Result The sequence analysis showed that the sequenced protein showed 99.9 % of homology with the AK amino acid sequence in Genbank .

氢化酶（hydrogenase，简称H2ase）是一类存在于微生物体内的重要生物酶，它可以催化氢的氧化反应，也可以催化还原质子产生氢气。

Hydrogenases are one of the important enzymes in many microorganisms , which can catalyze the reversible oxidation of hydrogen .

根据活性中心所含金属的不同，氢化酶可以分为三类：Fe-Fe（唯铁）氢化酶，Ni-Fe氢化酶和不含金属的氢化酶。

They are classified into three categories according to their active site metal ion , that is , Fe-only , [ Ni-Fe ] and metal free hydrogenases .

镍铁氢化酶活性中心的结构、催化机理及化学模拟

Active Site Structure , Catalytic Mechanism and Chemical Mimic of hydrogenase

化学模拟唯铁氢化酶活性中心

Chemical Mimic of the Fe-Only Hydrogenase Active Site

因此，唯铁氢化酶活性中心的化学模拟是本论文的主要研究内容。

Chemical mimic of the Fe-only hydrogenase active site is mainly discussed in this thesis .

氢化酶体是虫体的代谢产能重要细胞器。

The hydrogenosome was an important organelle for metabolism and producing energy of the parasites .

通过对一些结构特殊的模型配合物的研究，人们在实验室条件下重现了自然界氢化酶的某些特征。

Chemists have got some features of the hydrogenases existing in nature by studying specially designed model complexes .

利用高浓度有机废水生物制氢是环境工程重要的研究领域。本文对发酵产氢细菌产氢代谢的关键酶&氢化酶及其控制基因的研究现状进行了综述。

This paper reviewed the key enzyme-hydrogenase and it 's controlling gene of anaerobic bacterium during hydrogen bio-production .

在批量发酵中，这种复合添加剂使产甲烷量增加12.65%，氢化酶活力提高40.11%。

In batch experiment , the additive made CH4 production increasing 12.55 % and hydrogenase activity 40.11 % .

二种毛滴虫都有厌氧代谢产能的氢化酶体，而溶组织内阿米巴具有有氧代谢的呼吸细胞器微体。

Both trichomonas had hydrogenosomes of anaerobic metabolism and energy producing.E.histolytica contained microbody of aerobic metabolic breathing organelle .

取而代之的是，它们似乎拥有一种类似于氢化酶体的细胞器，这种细胞器与线粒体的作用类似，但它是通过厌氧作用为机体提供能量的。

Instead , they seem to have organelles that resemble hydrogenosomes-similar to mitochondria , but that use anaerobic chemistry .

含碱基[FeFe]氢化酶活性中心模型配合物的结构与性质研究

Studies on the Structures and Properties of the Complexes Containing Internal Bases of [ FeFe ] - Hydrogenase Active Site

其中，唯铁氢化酶的产氢活性是镍铁氢化酶的10-100倍。

The activity of H2 production of Fe-only hydrogenase is 10-100 times as high as that of [ Ni-Fe ] hydrogenase .

此外，某些酶是根据它们所催化的反应类型来命名的，例如磷酸化酶或脱氢化酶。

Also , some enzymes are named according to the type of reaction they catalyze , such as phosphorylase or dehydrogenases .

在吞噬与消化精子阶段，虫体的粗面内质网较丰富、高尔基体发达、氢化酶体数量较多。

The protozoa had plenty rough surfaced endoplasmic reticulum , well developed Golgi complex and many hydrogenosomes during active phagocytosis and digestion .

目前，主要的挑战是揭示唯铁氢化酶的催化机理以及寻求具有其催化活性的催化剂。

The major challenge is now to understand the enzymatic catalytic mechanism and search the synthetic competitive catalysts that function with hydrogenase-like capability .

对铁氢化酶活性中心进行化学模拟将有可能产生出新型的产氢催化剂。

Fe-only hydrogenase can catalyze the reduction of protons to molecular hydrogen and the oxidation of hydrogen to protons at extraordinarily high rates .

第三，分离纯化氢化酶蛋白并进行氨基酸测序，根据氨基酸顺序设计引物进行目的基因的克隆，或免疫抗体分离克隆目的基因。

The third , functional protein is isolated and primer of gene is designed by studying the sequence of amino acids for locating the gene of hydrogenase .

因其独特的结构特征和高催化活性，唯铁氢化酶活性中心的结构和功能模拟引起了生物无机化学家们极大的兴趣。

Because of the simple structure and high efficiency , biomimetic models related to the active site of the Fe-only hydrogenase are of particular interest to bioinorganic chemists .

光合细菌与产气肠杆菌协同产氢特性分析这种方法对于用氢化酶的兼性厌氧细菌或需氧细菌是有效的。

Characteristic Analysis of Cooperation Hydrogen Production Using Rhodopseudomonas sp. DT and Enterobacter Aerogenes This method is effective to the facultative anaerobic bacteria or aerobic bacteria with hydrogenase .

这两株光合细菌能利用多种简单有机物，并含有氢化酶，在废水净化及产甲烷方面有较广阔的应用前景。

These two strains have great potential in purification of waste water and production of CH4 , because they can utilize a few simple organic compounds and contain hydrogenase .

进行氢化酶控制基因的克隆的首选办法是进行相关物种氢化酶基因的网上查询，并设计引物克隆基因；

The first , the cloning gene of hydrogenase are that we should get the DNA sequence of hydrogenase in the related species and design the primer and clone the gene .

为研究氢化酶活性中心氮桥结构中氮原子是否起到质子摆渡的作用，设计了在分子内引入氢键的结构。

To explore the role of the bridged N atom for a possible proton transfer in the active site of hydrogenase , we tried to introduce a hydrogen bond into a couple of designed molecules .

利用微量热法和热动力学方程研究了过氢化氢酶反应。

Microcalorimetry and thermokinetic equations were used to study H2O2 decomposition by beef liver catalase .

本文主要介绍了磷脂的化学改性方法，例如：羟基化、氢化、酶水解、酰化等。

The methods of the modification of phospholipids were summarized , such as hydroxylation , hydrogenation , hydrolyzation , acetylation , and so on .

超声波氢化、电化学催化氢化和生物酶技术可以减少油脂中反式酸含量。

The ultrasonic hydrogenation , electro-chemical hydrogenation and enzyme technology can reduce the trans fatty acids in oils and fats .