昆布糖

昆布多糖的提取分离及F1的纯化与鉴定

Studies on extraction and isolation of polysaccharides from laminaria and purification and identification of F_1

目的：研究昆布多糖硫酸酯（LAMS）对急性髓性白血病HL-60细胞株的调控作用。

Objective : To study the effects of LAMS on modulation of HL-60 cell strain .

目的观察硫酸昆布多糖（laminarinsulfate，LS）对仓鼠胰腺癌肝转移模型的抑制效果，阐明其抑制肿瘤机制。

Objective To investigate the inhibiting effect of laminarin sulfate ( LS ) in hamster model of liver metastasis of pancreatic cancer and explore its mechanism .

昆布多糖（Laminarin）是昆布属、海带属等植物中含有的一种多糖类成分。

Laminarin is a polysaccharide composition contained in the genus Laminaria and other plants .

结果表明，昆布多糖最佳水浸提工艺条件为温度90°C、加水量60倍、浸提时间4h，多糖得率可达18.31%。

The results show that the optimum extraction process was : extraction temperature of 90 ℃ , water to-talling of sixty times , extraction duration of 4 h. The yield of polysaccharide was 18.31 % .

结果昆布多糖硫酸酯对HL-60细胞增殖有抑制作用，随着昆布多糖硫酸酯浓度的增加其抑制程度增强，在3.125μg/ml浓度下抑制作用达到最强；

Rasult : With the increase of LAMS concentration , the inhibiting effects on multiplication of HL-60 cell were increased . it is the most effects at 3.125 μ g / ml.

方法建立仓鼠胰腺癌肝转移模型，利用三种不同剂量LS对实验各组进行干预，应用昆布多糖、生理盐水作为对照组；

Methods After the hamster model of liver metastasis of pancreatic cancer was established , LS of 3 different dosages was given to animals in 3 experimental groups and sodium laminarin and normal saline were given to the animals in the 2 control groups , respectively .

昆布多糖提取工艺及理化性质分析

Optimizing extraction process and physicochemical property analysis of Ecklonia polysaccharide

昆布多糖对大鼠骨髓巨噬细胞生物活性的影响

Effect of laminarin on bioactivity of bone marrow macrophage

硫酸昆布多糖在仓鼠胰腺癌肝转移模型中抑制作用的研究

Inhibiting effect of laminarin sulfate in hamster model of liver metastasis of pancreatic cancer

结论硫酸昆布多糖对肿瘤生长、转移、血管生成有明显抑制作用。

Conclusions LS can significantly inhibit growth , metastasis and angiogenesis of pancreatic cancer .

在试验期内昆布多糖能明显改善高血糖大鼠的糖耐量，而对血清胰岛素水平的影响不明显。

TLP could significantly improve diabetic rat 's dose of sugar endurance , while had no obvious effect on the level of serum insulin .

酚氧化酶原显著地受血浆和昆布多糖同时存在的激活，但两者单独存在时对酚氧化酶原的激活作用较弱。

The ProPO was significantly activated by laminarin present in the plasma due to a much higher PO activity than that when the laminarin or the plasma was tested alone .

目的：对昆布多糖进行硫酸酯化，并对产物的纯度与结构进行分析，为研究其生物活性奠定物质基础。

Objectives : To sulfate the laminarin , then analyze the purity and elucidate the structure of polysaccharide sulfate , so as to make a foundation for the further research about its biological activity .