平板接种

方法：不加S9活化的条件下，用染料木素5、10、15、20μg/ml处理L5178Y细胞3h，采用微孔板法进行细胞毒性、平板接种效率和突变频率测定。

Methods : L5178Y mouse lymphoma cells were exposed to genistein at concentrations 5,10,15 and 20 μ g / ml for 3 hours without S9 metabolic activation . Determination of cytotoxicity , plating efficiency and mutant frequency were performed by using the microwell method .

方法采用96孔微孔板接种法检测平板接种效率和突变频率。

Methods The plating efficiency and the mutant frequency were detected by the microtiter procedure .

平板接种效率与氯仿作用时间的关系符合指数函数。

The correlation between the rate and the action time exactly filed the exponential function .

进行悬液定量杀菌试验时，采用酵母浸膏血平板浸润接种方法。

For suspension quantitative germicidal test , yeast extract blood plate infiltration inoculation method was used .

结果表明，用酵母浸膏血琼脂替代巧克力琼脂培养淋球菌无统计学差异，淋球菌用平板浸润法接种比平板涂布法好。

The results indicated that there was no statistical difference when yeast extract blood agar was used instead of chocolate agar for culturing gonococcus . Inoculation of gonococcus using plate infiltration method was better than that using plate smearing method .