基因重叠

与X基因重叠处变异的意义需进一步研究。

The importance of mutations in the X gene overlapping region needs to be further investigated .

通过引物设计，在抗脯氨酸反馈抑制耐盐突变菌株9315114的proBA基因重叠区引入一个限制性酶切位点，分别扩增出proB和proA基因，并构建融合的proBA基因。

A restriction enzyme site was inserted in the overlapping region of proB and proA genes from a salt-tolerant mutant of B. subtilis 93151 , and a fusion gene was constructed by cloning proB and proA genes respectively .

受生物基因片段重叠表达现象的启发，文中提出了一种新的基于重叠表达进化算法&MEOE（Multi-geneevolutionaryalgorithmbasedonoverlappedexpression）。

Inspired by the overlapped gene expression in the phenomena of biology , this paper proposes a novel evolutionary algorithm , MEOE ( Multi-gene Evolutionary algorithm based on Overlapped Expression ), and describes the genetic expression structures and relevant algorithm of it .

脆性部位中有12种与原癌基因位点重叠，重叠率为38.7%。

Among the fragile sites there were 12 which overlaped the proto oncogene sites and the superposition frequency was 38.7 % .

结论X基因区及重叠核心启动子区、增强子Ⅱ区多位点变异可能是HBV隐匿性感染的分子基础。

Conclusion Mutations of HBV in X gene and the overlapping core promoter , enhancer ⅱ may be the molecule bases of HBV occult infection .

RT-PCR扩增出VH和VL基因，经重叠延伸反应组装成单链抗体（ScFv）。

The VH and VL genes were amplified by RT-PCR , and ScFv was assembled by splicing overlap extension PCR .

该结果也暗示，根据QTL与差异表达基因的位置重叠来寻找致病性基因的成功率可能是很低的。

The results also implies that the the success rate of finding pathogenicity-related genes may be very low based on the positional overlapping between QTLs and differentially expressed genes . 2 .

有些基因之间有重叠，这样他们就不能单独的改变自己的功能了。

Some parts overlap , meaning that they cannot change jobs independently of one another .

方法用PCR方法扩增IL3信号肽序列和人内皮抑素基因，然后采用重叠延伸PCR拼接的方法将两者拼接和扩增。

METHODS IL_3 signal sequence and human endostatin gene were amplified with PCR and spliced with overlap extension PCR .

设计引物，扩增出抗对硫磷单链抗体的基因，并且用重叠延伸PCR的方法将其和核心链霉亲和素基因拼接，形成新的重组基因。

Designed primers to amplified the anti-parathion scFv gene , and then connect the gene with the core streptavidin gene by the method of overlapping extension PCR , we can obtain a new recombinant gene .

由于复杂突变的变异株不能产生功能性包膜蛋白，S基因与HBVdna聚合酶基因完全重叠，而这类变异株为优势毒株，其机制和意义值得深入研究。

Because of these deletion mutations , functional envelope protein could not be produced . S gene and HBV DNA polymerase gene are completely overlaps , but these variants were still predominant with mutated polymerase . The mechanism and significance were worth in-depth study .

S基因区未发现有义突变，X基因区及重叠核心启动子区、增强子Ⅱ区发现多位点变异，可能与HBV隐匿性感染有关。

Sequencing of the amplified fragments showed mutations in the region of X gene and the overlapping core promoter , enhancer ⅱ . No significant mutations were founded in the region of s gene .

本研究对两个候选基因列表进行了比较，发现两者间只有13个基因是重叠的，且其中多数属于Ⅱ型突变。

We compared the two gene lists and found that only 13 genes were overlapped between them , and most of these overlapped genes belonged to Type II mutation .

线粒体基因组含有13个编码蛋白质的基因，22个tRNA基因，2个编码rRNA亚基基因和两个非编码区域，部分基因有重叠现象。

The mtDNA genome has 37 genes , which includes 13 protein-coding genes , 22 tRNA genes and 2 genes for the rRNA subunits . A few of the genes are overlapped with each other , and two regions are noncoding .