固氮酶

固氮酶催化N2和H～+还原的机制

Mechanism of N_2 and H ~ + Reduction Catalyzed by Nitrogenase

pH和温度对固氮酶还原基质的影响

Effects of pH and temperature on substrate reduction by nitrogenase

N2键合在固氮酶M簇笼内的一个可能证据

A Proof for N_2-binding Inside M-cluster Cage of Nitrogenase

Mo、Co主要分布在籽粒中；蓝藻固氮酶钼铁蛋白的研究

Studies on nitrogenase Mo - fe protein of blue - green algae

固氮酶中N2和N2O结合位的一种新的鉴定方法

A New Method Identifying the Binding Site of N 2 and N 2O in Nitrogenase

模拟固氮酶中Mo微环境的化学探测

The Chemical Probe of Molybdenum - Micro - Surrounding on Nitrogenase

在pH≈9.0左右的溶液中，只有那些具催化活性的固氮酶模型系统，G2值降低。

In pH ≌ 9.0 media , only G_2 's of those nitrogenase model systems exhibiting catalytic activity were lowered .

重水中固氮酶催化还原乙炔产物的~1HNMR研究

1H NMR Study on the Products of the Catalytic Reduction of Ethyne by Nitrogenase in D_2O

应用群分解BHMO程序，研究了含S2的固氮酶活性中心模型和N2的络合活化模式。

In the present work , disulfide linkage-containing model of N2-ase active-center and probable coordination modes are studied by a group decomposition EHMO computation program .

用~1HNMR研究了固氮酶在重水中催化还原乙炔的反应产物氘代乙烯。

The products of the catalytic reduction ethyne by nitrogenase in D2O were studied by 1H NMR .

一种测定固氮酶含钼组分中~（99）Mo的γ射线计数器

A counter of recommendation for determing ~ ( 99 ) mo in containing mo & component of Nitrogenase

用乙烯为探针研究了固氮酶中N2的键合位。

Ethylene was used as a probe to detect the N2-binding site in nitrogenase .

固氮酶活性中心J系化学模拟物的合成、结构和性质研究

Studies on Synthesis , Structure and Properties of a Model Compound of J System Chemistry of Active Center of Nitrogenase

本文还扼要地讨论了固氮酶反应中ATP驱动的电子和质子传递机理。

Nitrogenase . A brief , further discussion of the mechanism of ATP-driven electron and proton , transports is also given .

以反应初速度计，其比活达4.1moleC2H4/分·moleMo，约为固氮酶活性的1%。

Its specific activity is 4.1 mole C_2H_4 / min · mole Mo which is about 1 % of the activity of natural nitrogenase .

ATP结合部位是固氮酶的调节部位；乙炔还原活性部位是固氮酶的催化部位；

ATP binding sites are regulatory sites in the nitrogenase and the acetylene reduction active sites are catalytic sites in the nitrogenase .

乙炔还原法测定出低固氮酶活性（0.17～3.43nMC2H4/株/小时）；

A low nitrogenase activity of the nodules was determined by acetylene-reduction method ( 0.17 ~ 3.43nMC_2H_4 / plant / hour );

根据固氮酶活性、分泌IAA能力等初筛出15株固氮菌，用于固氮菌盆栽单接种试验。

On the basis of nitrogenase activity and IAA secretion , 15 strains of nitrogen fixing bacteria were selected for single-inoculation pot test .

固氮酶活性中心MoKEXAFS谱拟合的初步探讨

Preliminary Investigation of MoK EXAFS Curve fitting for Active Site of Nitrogenase

相关分析发现，3种酶活性均同满江红的生产性能呈显著相关：固氮酶和POD酶活性呈正相关，SOD酶呈负相关。

The correlation analysis revealed that three tested enzymes had a significant correlation with Azolla productivity while nitrogenase and POD indicated the positive correlation and SOD indicated the negative correlation .

这都表明，水分胁迫对根瘤呼吸活性及ATP产生的抑制可解释干旱条件下固氮酶活性的下降。

These results suggest that it may be through the decrease in respiration rate and thereby that in the supply of ATP that drought stress inhibits ARA of nodules .

固氮酶活性中心模型的EHMO研究

EHMO Study of Nitrogenase Active-center Model

人工培养液中满江红的繁殖系数、固氮酶和过氧化物酶（POD）活性显著高于其它2种水体培养，而超氧化物岐化酶（SOD）的活性差异却不显著。

In artificial medium , Azolla had a significantly higher productivity , nitrogenase and peroxidase ( POD ) activity than in two natural water media but the superoxide dismutase ( SOD ) activities had little difference .

MSX对光合细菌GOGAT缺失突变株（Nif~-）固氮酶的诱导

The Induction of Nitrogenase by MSX in A GOGAT Mutant ( Nif - ) of Rhodopseudomonas sphaeroides

本文还讨论了ATP与[Fe4S4SPh）4]~（2-）原子簇的络合方式，进而探讨了ATP在固氮酶中的结合部位和作用机理。

Model of complexing ATP with [ Fe4S4 ( SPh ) 4 ] 2 - cluster is also discussed , and then complexing site and acting mechanism of ATP being in nitrogenase are proved .

固氮酶反应中铁蛋白与MgATP配位的化学模拟

Chemical Modeling of Complexation of Fe-Protein with MgATP in Nitrogenase Reactions

固氮酶Fe-Mo辅基模拟物Fe-Mo-S原子簇成键轨道的波函数图形分析

The Map Analysis of Bonding orbital Wave Function of Fe-Mo-S Cluster the Analogue of the Nitrogenase Fe-Mo-Cofactor

分别用来源于Av和Cp的固氮酶，研究了ATP/ADP和铁蛋白/钼铁蛋白（以下简写为Fe/MoFe）比对每传递2个电子时被水解的ATP分子数（即P/2e~-）的影响。

The effect of ATP / ADP and Fe / MoFe ratios on the number of ATP molecules hydrolyzed per electron pair transferred ( P / 2e-ratio ) was determined for nitrogenase from Azotobacter vinelandii and Clostridium pasteurianum .

固氮酶反应中ATP驱动电子传递的化学模拟&Ⅰ.[Fe4S4（SR）4]~（2-）原子簇与ATP络合的极谱及电子吸收光谱的研究

Chemical Modeling of ATP-driven Electron Transport in Nitrogenase Catalyzed Reactions ⅰ . The Studies of Complexing [ Fe_4S_4 ( SR ) _4 ] ~ ( 2 - ) Cluster with ATP Using Polarographic and Electronic Absorption Spectroscopic Methods

根瘤固氮酶活性以施用2mgSe/kg土的处理为最大，比对照增加了49.41%。

Root nodule nitrogen-fixing fermentation activity was higher with of application of 2.0 mg Se / kg soil , where it was 49.41 % higher than in control plot .