卵黄抗体

结论制备的抗SEA鸡卵黄抗体的特异性和敏感性均较高。

Conclusion Anti-SEA IgY with high specificity and sensitivity has been obtained and purified .

纯化的卵黄抗体分别置于6个EP管中，1～4号管的卵黄抗体仍有活性，第5管和第6管出现白色沉淀，可能是较高温度下蛋白变性引起的。

Purified IgY was added to six EP tubes , the number 1-4 still had partial activity , but number 5 and 6 showed some white precipitate , which was caused by protein denaturation at higher temperature .

ELISA法检测血清和卵黄抗体效价。

Both serum-and yolk-antibody titers were detected by indirect ELISA .

卵黄抗体不同pH值稳定性检测结果。

IgY stability at different pH.

特异性高免卵黄抗体对E.tenella的作用。

Tenella , effects of specific high immunized vitelline antibody on E.

应用斑点ELISA快速研究鸡卵黄抗体IgY的理化性质

Investigation of characteristic of egg yolk antibody using dot-ELISA

主要观察指标：①卵黄抗体SDS-PAGE电泳检测。

Main outcome measures : ① sds-page of IgY .

抗菌纳米SiOx粉体应用于PVC试验研究抗仔猪大肠杆菌病高免卵黄抗体粉的研制

Application of Microbicidal SiO_x Nanoparticles in PVC Research of Yolk Powder with High Level Antibody Against Colibacillosis of Piglets

在pH低于5或高于9的条件下37℃孵育3h后，卵黄抗体失去全部活性。

If pH was lower than 5 or higher than 9 , it lost the whole activity in above condition .

抗IBD高免卵黄抗体的研制

Studies on The Production of Yolk Antibody of Infectious Bursal Disease

SARS-CoV抗原免疫鸡后卵黄抗体效价变化研究

Change of yolk antibody titers in hens immunized with SARS-CoV antigen

抗脂蛋白脂酶卵黄抗体IgY的制备

Preparation of Chicken Egg Yolk Antibody ( IgY ) of Anti-Lipoprotein lipase

鸡新城疫卵黄抗体IgY的分离提纯研究

Study on the Extraction and Separation of Yolk Antibody IgY against Newcastle Disease

不同免疫方法制备IBD卵黄抗体的效果比较

Comparing the Results of the Preparation of IBD Yolk Antibody With Different Immunity Methods

鸡抗内毒素卵黄抗体IgY的制备

Preparation and screening of anti-endotoxin IgY in vitro

采用间接ELISA法和免疫荧光法对获得的卵黄抗体进行初步鉴定，其具有隐孢子虫属特异性。

The egg yolk antibody were examined with indirect immunofluorescence assay and indirect ELISA assay that has been set up , and testified it was against Cryptosporidium .

抗IBD高免卵黄抗体的研制性能价格比高。

Studies on The Production of Yolk Antibody of Infectious Bursal Disease high performance / cost ratio .

目的：了解SARS-CoV抗原免疫鸡后，卵黄抗体效价的变化。

AIM : To observe the change of yolk antibody titers after immunizing hens with SARS-CoV antigen .

硫糖铝对抗幽门螺杆菌重组VacA卵黄抗体的保护作用

Protective Effect of Sucralfate on Activity of IgY against Recombinant VacA of Helicobacter pylori

利用水稀释法和盐析法相结合，提取CCK卵黄抗体。

Using water dilution combined with salting out , we isolated the immunoglobulin from the egg yolk .

抗大肠杆菌O157∶H7鸡卵黄抗体的制备及其被动保护作用的研究

Study of preparation of yolk immunoglobulin against Escherichia coli O 157 ∶ H7 and its passive protective effect

鸡抗人IgG1卵黄抗体的制备、纯化和分析鉴定

Production , Purification and Determination of Chicken Egg Yolk Antibody against Human IgG1

二免后一周收蛋并用氯仿抽提法提取卵黄抗体，用间接ELISA法检测抗体效价。

One week after the second immunization , the eggs were collected and the yolk antibodies were extracted , then the titers of the yolk antibodies were detected by indirect ELISA .

卵黄抗体对ETEC粘附仔猪小肠上皮细胞的体外抑制作用研究

Adhesive inhibition of ETEC to piglets intestinal epithelial cells by egg-yolk antibodies against ETEC fimbriae in vitro

抗体通常是从免疫动物血液中获得的，卵黄抗体（IgY）指从免疫禽蛋中提取出的针对特定抗原的抗体。

Antibodies are usually produced from immunized animal plasma . Egg yolk antibody ( IgY ) against specific antigens are extracted from immunized birds .

浓缩病毒与IBD阳性血清和IBD高免卵黄抗体IgY琼扩反应呈弱阳性。

A weak precipitin line was observed between the concentrated virus and the IBD positive serum or IBD egg york antibody Igy in the AGP test .

抗鸡蛋黄IgY单克隆抗体的制备和初步鉴定抗猪产肠毒素大肠埃希氏菌卵黄抗体的研制及攻毒治疗试验

Preparation and Preliminary Characterization of Monoclonal Antibodies to Chicken Egg Yolk IgG Preparation and Application of Chicken Egg Yolk Immunoglobulins Against Enterotoxigenic Escherichia coli in Piglets

灌服和注射抗H.pylori卵黄抗体，可以抑制沙土鼠感染H.pylori，其抑制效果与抗菌素组无明显差异（p0.05）。

The egg-yolk antibody against H.pylori swallowed or injected might decrease H.pylori infection in the stomach of Mongolian gerbils , and the effect no obviously difference compared with antibiotic ( p0.05 ) .

鸡卵黄抗体的最佳ELISA操作参数为：包被抗原浓度为7.5μg/ml，封阻剂浓度为2.5%，抗体稀释倍数为1000，酶标二抗稀释倍数为14000；

The optimum ELISA parameters with IgY were that the concentration of antigen and blocking agent was 7.5 μ g / ml , 2.5 % and the dilution multiples of IgY and anti-chicken rabbit antibody-HRP were 1000 and 14000 , respectively .

利用HI和AGP方法检测普通种蛋中ND和IBD的卵黄抗体水平，利用HA和EID50检测方法比较多种病毒（如NDV、IBV、IBDV及AEV）在SPF鸡胚与普通鸡胚上繁殖性能的差异。

Detected the antibody level of common eggs with AGP method , compared the multiplication of several virus ( NDV 、 IBV 、 IBDV 、 AEV ) between SPF embryo and common embryo .