卵核

方法：应用SDSpage及考马斯亮兰染色技术对鸡卵核提取物进行蛋白组份分析；应用westernblot技术对鸡卵核蛋白抗原进行免疫识别分析。

Methods : The extracts from egg nucleus were analysed by SDS PAGE with Coomassie Blue staining and Western blot .

精核染色质在卵核内分散延续的时间长达4h左右。

The dispersing of sperm chromatin in egg nucleus takes about 4 hours ;

雌配子体中的极核和卵核遗传上是同一的。

The polar and egg nuclei of the female gametophyte are genetically identical .

目的：体内抗鸡卵核抗体（anti-eggnucleusantibody）是一类针对鸡卵核抗原的特异性抗体。

Objective : Anti-egg nucleus antibody is specific antibody to egg nucleus antigen in human serum .

不同年龄组、不同性别组的抗鸡卵核抗体均无显著性差异（P0.05）。

No significant differences of anti-egg nucleus antibody were found in groups with different age and gender respectively ( P0.05 ) . 3 .

结果用该技术分离纯化了鸡卵核中42000蛋白，获得约3000μg具有明显免疫反应性的高纯度抗原。

Results The 42000 protein was purified by this combined technique . The acquired purified protein antigen ( about 3000 μ g ) showed apparent immunogenicity and high purity .

异源MAR介导外源基因与家蚕受精卵核基质的体外结合

In vitro Association of Exogenous Gene with the Nuclear Matrix of Bombyx mori Fertilised Eggs Mediated by Heterogeneous MAR

以鸡卵核42000蛋白为抗原的斑点金免疫渗滤法的建立及对SLE血清学诊断的评价

Development and evaluation of a dot immunogold filtration assay for the serodiagnosis of systemic lupus erythematosus using 42-kD nuclear protein of chicken egg as antigen

方法1.随机挑选大连机车医院体检人群94人（男51名、女43名、平均年龄42.7岁），用ELISA方法检测随机人群中抗鸡卵核抗体的阳性率。

Blood samples were collected from random population in Dalian Locomotive Hospital ( 51 male , 43 female with average age of 42.7 ) . We used ELISA to detect the positive rate of anti-egg nucleus antibodies in serum . 2 .

授粉后18h，精核附在卵核的核膜上。6.授粉后20h，精核进入卵细胞核，精核染色质逐渐松解；

20 hours after pollination , sperm nucleus enters egg nucleus and male chromatin gradually disperses and 24 hours after pollination , male nucleolus appears .

结论：MCTD患者血清抗鸡卵核中72000蛋白抗体检测，对MCTD诊断有较高的价值，在治疗随访中也可起到一定的临床提示作用。

Conclusion : The detection of serum anti-72000 protein from egg nucleus antibodies is useful for diagnosis and follow-up of MCTD cases .

目的：分析鸡卵核提取物中检测混合性结缔组织病（MCTD）的特异性抗原成份。

Objective : To analyse the specific antigens extracted from egg nucleus as diagnostic antigens in patients with mixed connective tissue disease ( MCTD ) .

目的：通过检测混合性结缔组织病（MCTD）患者血清抗鸡卵核中72000蛋白抗体，研究其与MCTD有关指标的关系，以探讨该抗体在MCTD发病中的作用。

Objective : To study the correlation between anti-72000 protein from egg nucleus antibodies and the activity parameters of mixed connective tissue disease ( MCTD ) .

授粉后16h，花粉管进入胚囊，破坏1个助细胞，并将内含物释放入该助细胞内。精子与次生核融合比与卵核的融合快。

16 hours after pollination , one sperm nucleus moves to egg and enters it . The fusion of a sperm nucleus with a polar nucleus is faster than that with an egg cell .

SLE患者血清抗鸡卵核抗体的几何平均倒数滴度（GMRT）在患病的活动期为684.81±25.34，明显高于缓解期的404.53±16.63；

The geometric mean reciprocal titre ( GMRT ) of the anti-42000 protien from egg nucleus antibody in active SLE patients was 684.81 ± 25.34 , which was significantly higher than that ( 404.53 ± 16.63 ) during remission .

约34h，精核与卵核融合，并在卵核内出现分散的雄性染色质，进而出现雄性核仁；

In about 34 hours the egg nucleus and sperm nucleus begin fusing . In egg nucleus there is the male chromatin scattered , and then a small male nucleolus appears .

开花后5～10h，精核与卵核融合，雄性核仁出现，合子形成。

5 to 10 hours after flowering , the membrane of one male nuclear and that of the egg nuclear are fusing , and a small male nucleolus appears in the egg nuclear after fusing , as is the symbol of the formation of zygote .

抗鸡卵核抗体未表现有异嗜性。

Anti-egg nucleus antibody did not show heterophile . 6 .

卵核在卵的发育过程中变化很大。

With the development of the egg its nucleus changes a lot .

以鸡卵核为抗原建立了酶联免疫法，检验相应抗体。

Antibody to nuclear of egg was detected by ELISA coated with yolk .

90分钟，精子开始与卵核融合，初生胚乳核形成，其核中的三个核仁未融合；

Sperm and egg nucleus fusion and triple fusion took place at 90 m a p.

抗鸡卵核抗体参考范围与年龄、性别无关。

Reference limit of anti-egg nucleus antibody was not related with age or gender . 3 .

山羊卵核移植成功

Success in Goat Embryo Nuclear Transplantation

鸡卵核提取物中检测4种结缔组织病自身抗体的抗原分析

Analysis of antigens extracted from egg nucleus as detection of autoantibodies in patients with connective tissus diseases

山羊卵核移植的研究

Nuclear transplantation in goat embryos

异嗜性试验研究抗鸡卵核抗体是否具有异嗜性。

Heterophile test was used to study whether the anti-egg nucleus antibody has heterophile property . 6 .

13小时，卵核中的雌雄性核仁等大；

13 hours after anthesis , the female and male nucleoli in egg nucleus are the same size ;

精子与次生核的融合比与卵核的融合快。

The fusion of sperm nucleus with the polar nucleus is faster than that with the egg cell .

精、卵核融合时两核的核周腔内；

The perinuclear spaces of the egg and sperm nuclei at the fusion of the egg and sperm nuclei ;

混合性结缔组织病患者血清抗鸡卵核中72000蛋白抗体测定及其临床意义

The detection and clinical significance of ANTI-72000 protein from egg nucleus antibodies in patients with mixed connective tissue disease