单细胞培养

玉米细胞悬浮系建立和单细胞培养影响因素的研究

Establishment of maize cell suspension line and influencing factors to monocell culture of maize

草木樨状黄芪单细胞培养再生植株

Plant Regeneration From Monocell Culture of Astragalus melilotoides

用单细胞培养法，从人胃癌细胞系BGC-823中分离出10个克隆。

Ten cell strains were obtained from the human gastric cancer cell line BGC-823 by using the method of single cell cloning .

水稻细胞系的建立和单细胞培养再生植株

Establishment of rice cell line and Plantlets Regeneration from single cell culture

掌叶半夏细胞悬浮培养及单细胞培养再生植株

Suspension Culture and Single Cell Regeneration of Pinellia pedatisecta Schott

甘蓝型油菜游离单细胞培养及植株再生

Plaut Regeneration from Single Cell Culture of Brassica napus

水稻单细胞培养及植株再生

Rice single cell cultivation and Plantlets Regeneration

光棘豆单细胞培养再生植株

Plant Regeneration from Monocell of Oxytropis leptophylla

结论：组织块和单细胞培养法均可以从胚胎神经管分离、培养神经干细胞。

Conclusion : Embryonic neural stem cell can be obtained by both explant and monolayer culture .

小麦单细胞培养的研究&再生植株后代主要农艺性状变异及遗传稳定性

STUDIES OF SINGLE CELL CULTURE in vitro IN WHEAT Somaclonal variation and genetic stability of major agronomic characteristics from regenerated plants

本文主要应用单细胞培养技术培育紫菜新品系，以建立紫菜细胞工程快速育苗系统。

A new breeding technology with cell culture in Porphyra yezoensis was studied , to establish a system of fast breeding with cell engineering in Porphyra .

重型肝炎单核细胞培养上清和血浆TNFα含量的检测

A Survey of the Monocyte Cultural Supernatant and the Plasma TNF α Content of Severe Hepatitis

文中还探讨了建立悬浮培养细胞系的最适蔗糖浓度以及单细胞悬浮培养过程的细胞密度和培养液的pH值变化情况。

The optimum content of sucrose in medium for the establishment of the cell linos , the cell density in the culture process and the variations in pH of liquid are discussed .

结果：LPS直接刺激人牙髓细胞，牙髓细胞ALP活性明显上升，但LPS刺激的单核细胞培养上清对人牙髓细胞ALP活性有明显的抑制作用。

Results : ALP activity of pulp cell increased after three days of stimulation directly by LPS , while LPS stimulated monocyte culture supernatant markedly inhibited ALP activity .

为了研究丙烯酰胺的遗传毒理作用，采用单细胞克隆培养，双向筛选计数，多重PCR扩增与电泳分析，研究了诱导HL60和NB4两种细胞hprt基因突变率及分子突变谱。

The genotoxicity of acrylamide was investigated by methods of single cell clone culturing , two way screening count , multiplex PCR amplification and electrophoresis technique .

并采用定量夹心酶免疫吸附测定法检测单核细胞培养上清液中MMP9和MCP1的浓度。

MCP-1 and MMP-9 levels in monocytes culture supernatants were measured by enzyme-linked immunosorbent assays ( ELISA ) .

融合子F-20经单细胞分离培养，从20个单细胞中，筛选出一株生活力较强的单克隆菌株F-20-7，其细胞形态、大小、菌落特征均与葡萄酒酵母1450相似。

Single cell of fusant F-20 was separated and incubated .

结果：APS在体外可促进髓系多向造血祖细胞CFUGEMM增殖分化；APS诱导制备的骨髓基质细胞、内皮细胞、单核细胞培养上清液富含造血生长因子活性；

Results : APS and the conditioned media of bone marrow stromal cell ( BMSC ), endothelial cell ( Ec ), monocyte ( Mc ) induced by APS markedly promoted the proliferation and differentiation of early multipotential progenitor cell ( CFU GEMM );

内毒素刺激单核细胞培养上清对人牙髓细胞碱性磷酸酶活性的影响

Effect of lipopolysaccharide-stimulated monocytes culture supernatant on ALP activity of human pulp cell

红豆杉单细胞平板培养的研究

Study on the Monocell Plate Culture of Taxus Chinensis

绝经后骨质疏松与外周血单核细胞培养上清细胞因子的关系

Relationship between cytokines of peripheral blood mononuclear cells and pathogenesis of postmenopausal primary osteoporosis

磁化水在单细胞藻类培养中的应用初探

A primary study on magnetized water applied in the cultivation of single cell algae

基于响应面的泔水样培养单细胞蛋白培养基的优化

Optimizing Fermentation Medium of Food Wastes for Single-cell-protein Production by Method of Response Surface Analysis

棉胚珠来源单细胞的培养及部分特性初探

The Culture of Single Cells derived from Cotton Ovule and Preliminary Studies on Some of Its Characteristics

原生质体即利用酶解去除细胞壁的细胞，通常用于从细胞团中分离出的单细胞进行培养。

Protoplasts , cells from which the cell wall has been removed by enzymic digestion , are frequently used in cell culture to separate single cells from cell dumps .

外周血单核细胞培养上清肿瘤坏死因子α、白细胞介素1β及白细胞介素6水平：基础状态时，两组均较低，且差异不明显；

Levels of tumor necrosis factor - α, interleukin-1 β and interleukin-6 in PBMC : under basic state , those in the two groups were much lower and differences were not significant .

采用双抗体夹心酶链免疫吸附法，严格按照试剂盒说明测定外周血单核细胞培养上清肿瘤坏死因子α、白细胞介素1β及白细胞介素6水平。

Double antibody sandwich enzyme linked immunosorbent assay ( ELISA ) was used to measure the levels of tumor necrosis factor - α, interleukin-1 β and interleukin-6 in supernatant of PBMC strictly according to the instruction of kits .

结果鼠骨髓单核细胞培养体系中，加入载阿仑膦酸钠骨水泥微粒后，形成的破骨样细胞以及骨吸收陷窝数量明显少于不加或仅加入骨水泥微粒组（P<001）。

Results The addition of Alendronate-PMMA particles to mouse bone marrow monocytes cultures resulted in a marked decrease both in the number of TRAP positive , multinuclear cells and that of lacunar resorption pits compared with that no particles or PMMA particles only were added ( P < 0.01 ) .

成熟条斑紫菜叶状体酶解后获得单细胞，分别培养于添加了不同浓度N，P营养盐的0.5%和1.0%的琼胶培养基上，观察细胞的分化发育情况。

Cells enzymatically isolated from mature thalli of Porphyra yezoensis were cultured on 0.5 % and 1.0 % agar media , which were enriched with different concentrations of nitrogen and phosphorus respectively .

目的：探讨采用无血清专用培养基从肺癌患者外周血单核细胞快速培养树突状细胞（DCs）的方法。

Objective : To explore rapid generation of mature dendritic cells from peripheral blood mononuclear cells ( PBMC ) in serum-free medium .