别藻蓝蛋白

别藻蓝蛋白基因在毕赤酵母（Pichiapastoris）中的表达

Expression of allophycocyanin gene in Pichia pastoris

以重组别藻蓝蛋白表达工程菌JM109（DE3）/pET28-APC作为研究对象，对于JM109（DE3）菌株在乳糖诱导下表达重组蛋白的规律进行了研究。

The expression recombinant allophycocyanin gene in Escherichia coli JM109 ( DE3 ) using lactose as the inducer was optimized .

在工程菌株JM109中实现了带有6×His标记的新型重组别藻蓝蛋白HAPC的高效表达。

A new 6 × His · tag recombinant allophycocyanin HAPC was efficiently expressed in E.

从钝顶螺旋藻突变株（SP-Dz）中纯化别藻蓝蛋白（Allophycocyanion，APC）。

The Allophycocyanion ( APC ) was purified from a mutant of spirulina platensis ( SP-Dz ) .

别藻蓝蛋白ApcA2的体内生物合成

Biosynthesis of allophycocyanin ApcA_2 in vivo

含pET-28a/APC载体的大肠杆菌P4表达的重组别藻蓝蛋白HAPC，经金属螯合亲和层析法分离纯化，纯度达90%以上；

The recombinant allophycocyanin ( HAPC ) was expressed in E. coli P4 / pET-28a – APC . HAPC was purified by immobilized metal ion affinity chromatography and the purity reached above 90 % .

硫酸铵分级沉淀结合多种层析技术，从螺旋藻（SP-Dz）中纯化到电泳纯别藻蓝蛋白（Allophycocyanin，APC），纯度（A650/A280）达4·83。APC在30min内的荧光扫描曲线为直线；

APC of electrophoresis-purity was obtained from Spirulina using ammonium sulfate grade precipitation followed with several gel chromatography , and the purity ( A_ 650 / A_ 280 ) of which attained to 4.83 . The fluorescence scan curve of APC was a direct line in 30 min ;

富硒螺旋藻中硒别藻蓝蛋白的纯化及其特性

Purification and properties of Se-containing allophycocyanins from selenium rich Spirulina platensis

坛紫菜别藻蓝蛋白α，β亚基基因的克隆和序列分析

Cloning and sequence analysis of allophycocyanin genes from Porphyra haitanensis

新型重组别藻蓝蛋白亚基的表达和生物活性的初步研究

Expression of New Recombinant Allophycocyanin Subunits and Primary Study on Biological Activities

四种别藻蓝蛋白三聚体的时间分辨荧光光谱研究

Study on the Time resolved Fluorescence Spectroscopy of Four Kinds of Allophycocyanin Trimers

本文实现了重组别藻蓝蛋白基因在毕赤酵母中的异源表达。

In this paper , the heterogeneous expression of recombinant allophycocyanin in Pichia pastoris was completed .

基因重组别藻蓝蛋白对小鼠S（180）肉瘤的抑制作用

Elementary Study of Recombinant Allophycocyanin ( RAPC ) on S_ ( 180 ) Tumor in Mice