酵母人工染色体

含有His5基因用于酵母人工染色体重组筛选的置换型载体的构建

Construction of a Replaced Vector with His 5 Gene for Screening of Yeast Artificial Chromosome Recombination

目的？对人乳白蛋白酵母人工染色体（HLA-YAC）进行定点改造，以便进一步利用该载体作与基因表达调控相关的研究。

Objective : To modify human α - lactalbumin yeast artificial chromosome ( HLA-YAC ) and to use it as vectors for analysis of gene expression and regulatory elements of α - lactalbumin in vivo in the future .

酵母人工染色体DNA的大尺度物理图谱分析

Long range physical map of YAC DNA

利用酵母人工染色体（YAC）减数分裂同源重组构建人免疫球蛋白κ链基因组大片段

Reconstruction of large genomic segment coding for human immunoglobulin κ chain by meiotic homologous recombination of yeast artificial chromosome

转基因动物技术与YAC（酵母人工染色体）克隆及定位突变技术的结合可望阐述大片段基因（簇）中不同部位对发育阶段特异性表达的调控规律。

Moreover , yeast artificial chromosome ( YAC ) cloning and site-specific mutation techniques can be combined with transgenic technique to investigate the developmental gene regulation of large genes ( clusters ) with mutations in different sites .

酵母人工染色体（YAC）和细菌人工染色体（BAC）具有容量大的特性，可以将乳蛋白的整个基因座包括所有调控序列全部装载进去，有可能克服位点效应的影响，是一种理想的载体。

Given the high capacity of yeast artificial chromosomes ( YAC ) and bacterial artificial chromosomes ( BAC ), using YAC and BAC as transgenic vectors may be an ideal tool because they can contain all major important elements of lacto protein .

利用同源重组对酵母人工染色体左右臂进行多基因修饰

Modification of YAC Both Arms with Three Genes by Homologous Recombination

用Alu－VectorettePCR方法从酵母人工染色体末端分离人基因组单拷贝片段

Isolation of Human Genomic Single Copy from the YAC Insert End Using Alu-vectorette PCR

报导从酵母人工染色体的人基因组片段直接筛选表达顺序的方法体系。

This is the first report in China on the method of direct screening of coding sequences from human genomic YAC clones .

这些载体具有不同的特征，包括质粒、粘粒和酵母人工染色体。

These vectors can have different features ( such as the size of DNA-insert they can accommodate ) and include plasmids , cosmids , and yeast artificial chromosomes ( YACs ) .

着丝粒、端粒和复制起点是保持染色体在有丝分裂时稳定性的重要组分，利用酵母染色体的这些组分，1983年人们首次成功地构建了酵母人工染色体（YAC）。

Centromere , telomere and origins of replication are important elements to maintain the mitotic stability of chromosome . The Yeast Artificial Chromosome ( YAC ) was firstly built in 1983 triumphantly by using these elements of yeast .