转录终止
- transcription termination;termination of transcription;transcription pausing
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线粒体转录终止因子蛋白的作用是与线粒体DNA的特异位点结合,导致线粒体基因转录停止。
The function role of mitochondrial transcription termination factor is binding mitochondrial special DNA and leading to terminate mitochondrial gene transcription .
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TCS基因下游为转录终止信号。
A transcription termination signal was found downstream of TCS gene .
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提供转录终止信号的DNA顺序称为终止子。
The DNA sequence that provides the signal to cease transcription is called a terminator .
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真核mRNA转录终止机制
Eukaryotic mRNA Transcriptional Termination Mechanism
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酶切鉴定、PCR鉴定和测序结果表明,成功获得了TK基因,获得的TK基因内存在一个KpnⅠ酶切位点和编码区的早期转录终止信号TTTTTN(T)T。
Restriction enzyme assay , PCR and sequencing confirmed that the TK gene was obtained successfully . Only one Kpn ⅰ site and a transcription termination signal TTTTT N ( T ) T were found in the gene .
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在设计的靶序列5′端开始的19个核苷酸为正义链,中间加入9个核苷酸TTCAAGAGA的间隔以形成发夹结构,3′端加有转录终止信号TTTTTT。
The sense strand of siRNA was composed of the start 19 nucleotides of the 5 ' end of target sequence .
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内源性终止子是凭借自身的序列信号即可实现转录终止功能的调控元件。
Intrinsic terminators are sequence elements which can terminate transcription in the absence of any additional factors .
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还探索了转录终止序列对融合基因蛋白表达水平的影响,构建了高效表达融合蛋白的载体-宿主系统。
Based on the results , a vector-host system was established for the high expression of fusion proteins .
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由tRNA引导的转录抗终止机制普遍存在于革兰氏阳性细菌中,调控氨酰tRNA合成酶基因和与氨基酸合成有关的酶基因的表达。
A tRNA-directed transcription antitermination mechanism is common in Gram-positive bacteria , regulating the expression of aminoacyl-tRNA synthetase and amino acid biosynthesis genes .
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nutR中的突变对λ噬菌体早期转录抗终止作用的影响
Mutations in nutR Affect Early Transcription Antitermination in Bacteriophage λ
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生精细胞中活跃的转录活动,终止于圆形精子细胞向长型转变的过程之初。
Transcription in mammalian spermatogenesis is highly active until mid-stage of spermiogenesis when it suddenly ceases .
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但对核小体结合模体的相对偏好性进行比较后发现,转录起始位点、转录终止位点、起始密码子和终止密码子附近±500bp的序列所包含的偏好的核小体结合模体模式数各不相同。
Comparison of relative preference of nucleosome binding motifs shows that the mode number of preferred nucleosome motifs in ± 500bp region around the transcription start sites , transcription termination sites , start codon and stop codon is different .
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分析的功能位点包括:转录起始位点,转录终止位点,起始密码子,终止密码子,内含子和外显子连接处等。
Analyzed functional sites contain transcription start sites , transcriptional termination sites , start codon , stop codon and junction sites of intron and exon .