超速离心

采用不同pH值沉淀法及超速离心获得提纯的水稻草矮病特异蛋白。

The disease-specific protein of rice grassy stunt virus ( RGSV ) was purified by differential pH precipitation and ultracentrifugation .

本文应用超速离心、凝胶过滤层析、高效液相色谱法分离纯化了人血清极低密度脂蛋白&载脂蛋白CⅠ和CⅢ。

Apolipoprotein C ⅰ, C ⅲ were isolated and purified by ultracentrifugation , gel filtration and high performance liquide chromatography from human serum very low density lipoprotein .

方法：采用多聚阴离子沉淀结合短时密度梯度超速离心法得到纯化的LDL；

Methods : To isolate LDL by using polyanions precipitation and short-time density gradient ultracentrifugation .

TGEV细胞培养物超速离心与RT-PCR扩增的研究

Ultra Centrifugation the Cell Cultures of TGEV and RT-PCP

用超速离心纯化的乙型脑炎病毒抗原，建立了间接ELISA方法检测乙脑IgG抗体。

With purified Japanese encephalitis ( JE ) virion , an indirect ELISA was developed to detect antibody of IgG class against JE .

结论：采用超滤、PEG沉淀及连续蔗糖密度梯度超速离心的方法可制备满足麻疹IgGEIA诊断试剂要求的包被抗原。

Conclusion : We can prepare the coating antigen in measles IgG EIA test system by continuous sucrose density gradient ultracentrifugation .

兔血清LDL、VLDL及HDL用密度梯度超速离心法分离得到。

The serum LDL , VLDL and HDL were isolated by the density gradient ultra centrifugation .

目的研制载脂蛋白A1和B检测试剂。方法采用硫酸葡聚糖沉淀、超速离心、层析等方法。

Objective To prepare a kit for detecting apoprotein ( Apo ) A1 and B. Methods Dextran sulfate precipitation , ultracentrifugation , chromatography , and so on .

CsCl超速离心结合蔗糖密度超速离心可纯化此蛋白。

This protein could be purified with CsCl and sucrose density gradient ultracentrifugation .

脂蛋白及载脂蛋白对脂蛋白受体作用的研究&Ⅰ.一步超速离心分离血清VLD.LDL及部分HDL和LDS

Studies on Effect of LiPoProteins and APoProteins on LiPoProtein Receptors ⅰ . Isolation of LiPoproteins and LDS by One Step Ultracentrifugation

方法：采用密度梯度超速离心法分离不同密度LDL亚组份；分析冠心病（CHD）患者和健康人群LDL亚组份分布特征。

Methods : LDL subfractions were isolated directed from plasma by non equilibrium density gradiant centrifugation , and distribution of LDL subfractions in CHD patients were analyzed .

方法超速离心法制备肝微粒体，放射性同位素方法测定肝脏HMGCoA还原酶的活性。

METHODS The liver microsomes were prepared by ultracentrifugation , the HMG CoA reductase activity was determined by radioactivity in vitro .

分别采取PEG-6000沉淀法、酶直接消化法和超速离心法浓缩、纯化减蛋综合征病毒，并提取基因组DNA。

PEG-6000 precipitation , direct proteolysis and ultra centrifugation were used to purify and extract genomic DNA ( gDNA ) from egg drop syndrome virus ( EDSV ) respectively .

方法：应用超速离心法分离胆石症患者胆汁泡，运用SDS-PAGE电泳分离其中胆汁泡蛋白，并经HPLC系统纯化，研究其成核活性和N-端氨基酸序列。

Methods : Biliary vesicules were isolated by ultracentrifugation , and their vesicular protein ( s ) isolated by SDS-PAGE and purified by HPLC . The nucleating activity was determined by the nucleation-time method .

采用超过滤、PEG沉淀和超速离心综合步骤对MT-5细胞培养上清中的HBsAg进行纯化。

The purification of HBsAg in MT-5 cells culture supernatant was carried out by three different steps including ultrafiltration , precipitation by PEG 6000 and three-step ultracentrifugations .

通过差速离心法和蔗糖密度梯度超速离心后所得的提纯病毒样品，用紫外分光光度法检测可见典型的病毒核蛋白吸收曲线，用SDS-PAGE分析和电镜观察也证明该样品为病毒纯品。

The purified virus sample gained by sucrose density gradient centrifugation was proved to be typical absorbance curve of virus nucleoprotein and pure virus by methods of SDS-PAGE and electron microscopy and so on .

方法：应用中空纤维超滤、超速离心、层析和电泳等方法，对肺癌及卵巢癌患者的胸腔积液、腹水中TAF进行提纯和分析；

METHODS : TAF was purified at scheduled intervals by using extract methods such as hollow fiber column , ultracentrifugation , chromatography and electrophoresis .

但本法省去了常规超速离心分离人血清极低密度脂蛋白（VLDL）的过程，只需微量血清，便于临床应用。

This method however , omits the step of ultracentrifugation to isolate VLDL and needs only a few microliters of serum ; it is convenient for use in clinical diagnosis .

无基质猪血红蛋白（SFH）由新鲜猪血溶血制得并经超速离心。

Stroma-free porcine hemoglobin ( SFH ) was separated and purified from fresh pig blood by centrifugation and filtration with 0.45 to 0.22 u m films .

本法与常规法比较。不需要超速离心分离人血清VLDL的过程，而且仅需微量血清，便于临床应用。

Compared with conventional method , the present method can be readily used for clinical detection as it only requires microliters of serum and does not need ultracentrifugation for isolation of VLDL .

方法：本研究以人巨细胞病毒（HCMV）包膜蛋白和皂苷类佐剂QuilA作用，采用非离子去垢剂Mega－10及超速离心技术，进行了HCMVISCOMs实验型疫苗的制备。

Methods : In this study , the envelope proteins of HCMV were complexed with the glycoside Quil A by hydrophobic interaction . Mega 10 and sucrose gradient centrifugation were also used .

经红外光谱、超速离心及电镜观测可知：银耳多糖铁（Ⅲ）配合物是以β-（FeOOH）。

The investigation by infrared spectra , ultracentrifugation and electron microscopy showed that structure of ITC was an interracial compex with β - ( FeOOH ) .

为研究LDL的理化性质及其功能，研究人员发明了多种LDL分离技术，如超速离心、化学沉淀、凝胶过滤层析及亲和层析等，但这些技术均有不足。

In order to study the physical-chemical properties and functions of LDL researchers have invented a variety of LDL separation methods , such as ultracentrifugation , chemical precipitation , gel filtration and affinity chromatography . However , each of them has its disadvantages .

方法：大鼠分别灌服给予相当于生药量10g/kg、2g/kg菊花提取物，连续15天，用超速离心法制备肝微粒体，用紫外分光光度法测定细胞色素P450及其同工酶的活性。

Methods : The rats were given orally the extracts in the doses of 10g / kg and 2g / kg ( equivalent to the crude herb ) for 15 days respectively , and then liver microsomes were prepared with ultra-centrifuge method .

分析超速离心测定，病毒粒体为单一沉降组分，S20.w约73S。

The purified virus shared a single sedimenting component of 73S ( S20 . w ) in analytical ultracentrifugation .

该菌株在光照条件下，38℃摇瓶培养6d，可用高速冷冻离心、透析袋、蔗糖密度梯度纯化、超速离心等得到紫膜。

The strain was cultured under light treatment , temperature 38 ℃, shake cultivation 6 days , the purple membrane was gained through high speed freeze centrifugation , dialysis , sucrose density gradient centrifugation , superspeed centrifugation etc.

H-16电泳分析RNA及外壳蛋白质均只形成一条带，其分子量分别为2.9×10~6-3.2×10~6及26000-26500.分析超速离心只出现一个峰。

By electrophoresis , RNA and coat protein of H-16 showed only one band respectively , the molecular weight of RNA was 2.9-3.2 × 10 ~ 6 while the MW of coat protein was 26,000-26,500 . By analytical ultracentrifugation also only one peak appeared .

将复原脱脂乳在70～90℃范围内加热10～25min后，用超速离心分离出酪蛋白微粒，并用毛细管电泳法定量分析。

Reconstituted skim milk samples were heated at temperatures ranging from 70 ~ 90 ℃ for 10 to 25 minutes . After ultracentrifugation casein micelles were seperated and analyzed by capillary electrophoresis .

方法：以山羊胎皮为原料，通过112%硫酸铵及10%氯化钠盐析和超速离心，DE32和DE52阴离子交换纤维素柱层析提取纯化得到羊Ⅲ型前胶原。

Methods : Purified gPC ⅲ was acquired from the material of goat fetal skin through salting out ( 11.2 % ammonium sulfate and 10 % sodium chloride ), ultracentrifuge and ion-exchange cellulose chromatography ( DE32 and DE52 ) .

蔗糖垫超速离心法提纯乙型脑炎疫苗

Purification of Japanese Encephalitis Vaccine by Ultracentrifugation with Sucrose Bed