质粒

空肠弯曲菌质粒DNA提取条件的优化

A modified extraction method of the plasmid DNA in Campylobacter Jejuni

小量提取质粒DNA的简易方法

A Simple Method Of Extracting Plasmid DNA On a Small Amount

结果：pcDNA3.1-IL-10酶切鉴定的电泳结果显示，pcDNA3.1-IL-10质粒有一个560bp左右的插入片断，大小和IL-10cDNA大致符合

IL-10-cDNA fragment was obtained through cleaving pUC-T-IL-10cDNA by reconstriction enzymes .

质粒DNA快速提取试剂盒（博大公司）；

Kits for rapid extraction of plasmid DNA ( Boda Company );

用Red介导的同源重组结合酶切改构质粒DNA

Reconstruction of plasmid DNA with Red-mediated homologous recombination combined with restriction digestion

碱裂解法提取质粒DNA的研究

The Compare Study on the Method of Extracting Plasmid DNA

并用裸DNA质粒经肌肉接种鸡体内表达。

Chickens were immunized intramuscularly with the naked DNA plasmid .

大肠杆菌质粒DNA提取方法的优化

A Modified Method for Isolation of Plasmid DNA from E.coli

通过质粒双酶切和DNA测序证实该重组质粒构建正确。

This was confirmed by cleavage of restriction endonuclease and DNA sequencing .

在碱解法的基础上，优化了提取空肠弯曲菌质粒DNA的条件。

The conditions of extracting plasmids DNA in Campylobacter jejuni were optimized .

一种从质粒DNA中去除细菌染色体DNA污染的方法

A method of eliminating chromosomal DNA from contaminated plasmids DNA

快速密度梯度离心法纯化质粒DNA

Purification of plasmid DNA by rapid density gradient centrifugation

对重组质粒DNA进行纯化，并对目的基因片段进行核苷酸序列分析。

The recombinant plasmid was purified and the target DNA fragment was sequenced .

建立一种全质粒DNA序列测定方法

To construct a method of whole plasmid DNA sequencing

重组质粒DNA中插入子的核苷酸序列分析

Nucleotide sequence analysis of inserts in recombinant plasmid DNA

原子力显微镜研究不同温度下质粒DNA结构变化

Plasmid DNA structures in different rangs of temperature studied by atomic force microscopy

大质粒DNA提纯方法的改进

Improved Method for the Extraction of Large Plasmid DNA

连续热裂解法大规模制备质粒DNA

Large-scale Plasmid DNA Preparation by Flow-Through Thermal Lysis

医院内耐药细菌质粒DNA的实验研究

Experimental research of plasmid profile of drug resistance

提取质粒，基因组DNA进行PCR扩增测序。

Plasmid was extracted and genome DNA was sequenced by PCR amplification .

激光辐照对离体质粒DNA的断裂效应

Effect of Laser Radiation on Single and Double Strand Breakage of in vitro Plasmid DNA

不锈钢血管支架蛋白涂层和携带质粒DNA的研究

Methodological Studies of Protein Coating and Plasmid DNA Delivery Based on Stainless Steel Coronary Stents

四川省规模化猪场大肠杆菌质粒DNA分析

Plasmid DNA analysis on E coli from the large scale of pig farm in Sichuan Province

TGF-β1质粒DNA前房注射在角膜内皮细胞表达的实验研究

Expression of Plasmid DNA Encoding TGF - β _1 in Endothelium after Injection into the Anterior Chamber

基于Vector类向量运算的碰撞侦测研究将其克隆到E.coli质粒上进行序列分析。

Analysis of Collision Detection Based on Vector Quantities coli vector .

扩增产物经纯化后直接克隆到PMD18-T载体系统中，经PCR及酶切法鉴定，所得的重组质粒中含有495bp的片段。

After purification , the DNA fragment was cloned into PMD-18T vector directly .

鼠疫菌66Mdal质粒与鼠毒素及F1抗原的关系

The relationship between plasmids of y.pestis , and murine toxin & F1 antigen

方法：质粒提取及PCR扩增。

Methods : Plasmid extraction and PCR amplification .

经研究发现纯化的质粒不含RNA和蛋白质污染；

The purified plasmid DNA did not contaminate with RNA and protein .

我国类鼻疽流行病学调查云南省鼠疫疫源地鼠疫菌质粒DNA种类及分子流行病学研究

Study on the Category of Plasmid DNA and Molecular Epidemiology of Yersinia pestis Isolated from Yunnan Natural Plague Foci