角膜缘干细胞

角膜缘干细胞移植术与结膜移植术治疗外伤后假性胬肉及复发性胬肉疗效的比较

Comparison of transplantation of corneal limbal stem cell with conjunctival transplantation for the treatment of pseudopterygium and recurrent pterygium

目的分析DispaseⅡ酶对人角膜缘干细胞培养的影响。

Objective To analyze the effect of Dispase ⅱ on human limbal stem cell culture .

目的：评价自体角膜缘干细胞移植联合丝裂霉素C在翼状胬肉治疗中的临床疗效。

Objective : To observe autologous stem cells transplantation of limbus corneae combining mitomycin C to treat pterygium .

低浓度丝裂霉素C联合自体游离角膜缘干细胞移植治疗原发性翼状胬肉的研究

The Study of Low-dose Mitomycin C in the Corneal Limbal Stem Cell Autografting for Prevention of Primary Pterygium

B组37眼行翼状胬肉切除+角膜缘干细胞移植；

Pterygium was ablated and limbal stem cells auto-graft transplantation was performed in group B ( 37 cases ) .

丝裂霉素C联合自体角膜缘干细胞移植治疗翼状胬肉

Clinical study of auto-corneal limbus stem cell transplantation combined with Mitomycin C ( MMC ) for the treatment of pterygium

结论丝裂霉素C联合自体角膜缘干细胞移植术、丝裂霉素C联合带蒂结膜瓣转移术是治疗复发性翼状胬肉较理想的手术方法。

Conclusion Mitomycin C plus corneal limbal stem cells autografting and mitomycin C plus transference of pedicled conjunctival flap are two ideal therapeutic methods for recurrent pterygium .

EGF，bFGF单独及联合应用对原代培养的兔角膜缘干细胞增殖的影响

Effects of EGF and bFGF on proliferation of primary cultured rabbit corneal limbal stem cells

目的比较自体角膜缘干细胞移植术、丝裂霉素C联合自体角膜缘干细胞移植术、丝裂霉素C联合带蒂结膜瓣转移术的临床疗效。

Objective To investigate the therapeutic effect of corneal limbal stem cells autografting , mitomycin C plus corneal limbal stem cells autografting and mitomycin C plus transference of pedicled conjunctival flap .

方法将79例（87眼）翼状胬肉患者随机分为A组（带角膜缘干细胞自体结膜瓣移植术）和B组（单纯胬肉切除术）。

Methods 79 cases ( 87 eyes ) of pterygium were randomly divided into two groups , Group A ( autologus conjunctival limbus transplantation ) and Group B ( simple excision of ptergium ) .

目的观察低浓度丝裂霉素C（MMC）在自体游离角膜缘干细胞移植术治疗原发性翼状胬肉的临床疗效。

Objective To investigate the clinical effect of low-dose mitomycin C ( MMC ) in the corneal limbal stem cell autografting for prevention of primary pterygium recurrence .

用EDTA去除饲细胞，间接免疫荧光染色检测角膜缘干细胞克隆团表达增殖细胞核抗原（PCNA）情况。

After feeder cells were discarded by0.04 % EDTA , the cultivated limbal stem cells were examined with immunofluorescence staining of PCNA .

对人自体角膜缘干细胞进行体外培养，倒置显微镜观察培养细胞体外生长的形态，对培养出的细胞用抗角蛋白K3的单克隆抗体AE5进行鉴定。

The growth of cultured cells in vitro were observed under inverted microscope .

结论体外单独及联合应用EGF与bFGF对原代培养的兔角膜缘干细胞的增殖能力有明显的促进作用，为提供大量的角膜缘干细胞以治疗眼表疾病提供了实验基础。

Conclusion Application of EGF or bFGF alone and their combined application in vitro can strengthen the proliferation of cultured corneal limbal stem cells .

方法：A组37例（48只眼）翼状胬肉（其中11例为复发性翼状胬肉）在手术显微镜下实施翼状胬肉常规切除联合丝裂霉素C（MMC）的治疗，然后行自体角膜缘干细胞移植。

Methods : Group A , 37 cases ( 48 eyes )( including 11 recurrent pterygiums ) were operated with pterygium excision , combining mitomycin C , followed with autologous stem cells transplantation of limbus corneae ;

原代培养角膜缘干细胞与ΔNp63蛋白表达的年龄相关性研究

Age-related study on primary culture of limbal stem cells and the expression of Δ Np63 protein

纽卡斯尔大学（newcastleuniversity）研究出来的这一疗法，可帮助数以千计因“角膜缘干细胞缺损”（lscd）而导致视力严重受损的人。

The treatment , which has been developed at Newcastle University , could help thousands of people who suffer severely impaired vision through a condition known as limbal stem cell deficiency .

目的探讨羊膜移植（AMT）重建眼表治疗角膜缘干细胞缺陷（LSCD）的临床价值。

Objective To evaluate the appling value of amniotic membrane transplantation ( AMT ) in comeal surface reconstruction for patients with limbal stem cell deficiency ( LSCD ) .

结论：NGF可有效促进培养角膜缘干细胞的生长，培养的适宜浓度为10~100ng/ml，为角膜缘干细胞移植患者术后及角膜外伤或溃疡时局部应用NGF提供了理论依据。

Conclusion : NGF enhances the proliferation of the stem cells in vitro effectively . The optimal concentration ranges from 10ng / ml to 100ng / ml. The experiment provides the theoretical basis for using NGF for surgical transplantation and for patients suffering from corneal trauma or ulcer .

结果胎儿角膜缘干细胞原代培养时生长旺盛，8~10d达到生长高峰，CFE为（26.18±4.75）%，传代时保持高增殖速率；

Results Fetal limbal stem cells grew swiftly and reached growth peak in 8 ~ 10 days in primary culture when their CFE was ( 26.18 ± 4.75 ) % . They could keep high proliferating rate in subculture .

成人角膜缘干细胞原代培养时生长不及胎儿细胞旺盛，6~8d达到生长高峰，CFE为（15.45±3.88）%，随着传代次数增加其增殖能力明显下降；

Adult limbal stem cells grew less swiftly than fetal cells and reached growth peak in 6 ~ 8 days in primary culture when their CFE was ( 15.45 ± 3.88 ) % . Moreover , their proliferating ability declined distinctly along with the passage times increasing .

兔角膜缘干细胞两种培养方法的比较

Comparison of two different culture techniques of rabbit limbal stem cells

制作角膜缘干细胞完全缺乏动物模型的新方法

New method to creat rabbit model of limbus stem cell deficiency

人角膜缘干细胞初步建系研究

A Primary Establishment and Study of Human Limbal Stem Cell Lines

外源性基因在兔角膜缘干细胞中的表达及意义

Expression and significance of exogenous gene in rabbit limbal stem cells

带角膜缘干细胞的板层角膜移植术治疗严重角膜病变

Lamellar Keratoplasty with Limbal Stem Cells for Treating Serious Corneal Diseases

角膜缘干细胞的鉴定、筛选与培养的研究进展

Identification , Purification and Culture of Limbal Epithelial Stem Cells

带蒂角膜缘干细胞移植治疗翼状胬肉临床观察

Autograft transplantation of pedical limbus cornea stem-cells for treating pterygium

翼状胬肉与角膜缘干细胞缺乏的病理研究

A Pathological study of the limbal stem cells in the cervical pterygium

角膜缘干细胞体外培养体系的建立

Establishment of in Vitro Culture System of Limbal Stem Cells