血琼脂

空肠弯曲杆菌进入活的非可培养（VBNC）状态后，在正常的哥伦比亚血琼脂培养基中不能生长繁殖。

While campylobacter jejuni come into the viable but non-culturable ( VBNC ) state , it can not cultured in normal Columbia Blood Agar medium .

结果，淋球菌在普通营养琼脂和酵母浸膏琼脂上生长的菌落均小于2mm；在酵母浸膏血琼脂和巧克力琼脂上生长的菌落为2~3mm，计数回收率高出26%。

Results : The colonies of gonococcus growing on ordinary nutrient agar and yeast extract agar were smaller than 2 mm , while those growing on yeast extract blood agar and chocolate agar were 2 ~ 3 mm and the recovery rate was 26 % higher .

将液体培养物稀释到同一梯度，定量接种于固体培养基上（绵羊血琼脂平板和特殊培养基），分别进行需氧和厌氧培养24~48h。

Things of the above cultured , after diluted to the same gradient and inoculated on solid culture mediums quantitatively , were cultured for 24 ~ 48 h under the condition with oxygen and without oxygen .

在血琼脂平板中疏肝和胃丸抗幽门螺杆菌测得MIC为0.0156g/ml生药，其抑菌作用随着药物浓度的增高而增强。

The minimum inhibitory concentration in above stated plate was 0.0156g/ml . and the anti-bacteria function was increasing with the augment of drug concentration .

方法应用改良血琼脂培养基，对326例非特异性阴道炎患者的阴道分泌物进行阴道加德纳菌分离培养，同时与快速细菌性阴道病的检测新方法BV-fast及BV-blue进行比较。

Methods Vagina secretions of 326 patients with general vaginitis were cultured on improved blood agar culture media , and meanwhile compared with fast-testing assays of bacterial vaginosis BV-fast and BV-blue .

对其进行紫外线诱变和亚硝基胍诱变复合处理，从血琼脂平板上筛选出一株不具有溶血性的突变菌。

A mutant without haemolysis was screened from blood agar plate .

用五种培基对淋病奈氏菌生长作比较观察，其中，肝消化血琼脂效果令人满意。

A comparative observation was made for the growth of Neisseria gonorrhoeae in .

改良血琼脂的评价与应用

Evaluation and Application of an Improved Blood Agar Medium

仅依据菌落形态的符合和在血琼脂上不生长初判军团菌；

Initial identification was only relied on colony appearance but no growth on the blood agar plate ;

研究以平菇汤为基础成分，代替动物组织提取物，制备平菇液体培养基，平菇血琼脂培养基。

Mushroom broth is used to replace animal tissue extract to produce mushroom culture solution and mushroom blood agar medium .

该菌在心脑血琼脂平板上生长良好，在血清琼脂、普通血琼脂、空弯血琼脂上几乎不生长。

Better growth was obtained on the brain-heart blood agar ; almost no. growth occured on the serum agar , normal blood agar and C.

方法：采用鸡蛋斜面培养基，亚碲酸钾血琼脂平板和肉汤培养基观察其生长特性、测定多种生化反应性、毒力和药物敏感性。

Methods : To observe the cultural characteristics with the medium of inclined plane of egg , agar plate with blood and potassium tellurite , and broth .

方法采用改良布氏无血琼脂培养基以及改良弯曲菌蛋黄培养基在相同条件下培养空肠弯曲菌，观察其生长状况及生物学性状。

Methods For measuring the bacteric growth and biochemical characteristic , Campylobacter jejuni is cultured in improved Bull 's no blood agar and the improved medium containing yolk .

采用选择性哥伦比亚血琼脂为分离培养基，通过革兰染色镜检、尿素酶试验和氧化酶试验对分离的幽门螺杆菌进行鉴定。

Columbia blood agar was used as selective medium to isolate H. pylori . Gram-staining microscopy , urease test and Oxidase test were applied to identify the isolated bacteria .

方法将标准菌株接种血琼脂，取菌苔分别接种克氏双糖铁琼脂、硝酸盐培养基、尿素培养基、蛋白胨水、明胶液化培养基，37℃培养，观察结果。

Methods KLIGLER agar , nitrate broth , urea agar , peptone water , nutrient gelatin were inoculated with lawn of 3 standard strains , then incubated at 37 ℃ .

实验组液体33瓶，在细菌实验室将实验组液体的塑料瓶盖开启后，直接在瓶塞上采样，将所取标本接种于无菌血琼脂平板培养基内，观察对照组和实验组的细菌生长情况。

Study group 33 of liquid , plastic lid of bottle opened and disinfect the bottle cork , sample immediately , vaccinated those sample into asepsis agar plate medium and observed it .

平菇血琼脂培养基对140份痰及中段尿标本阳性菌的检出率为34.3%，传统血琼脂培养基对阳性菌的检出率为32.1%。

For 140 case of sputum and urine culture , the positive rate on mushroom blood agar medium is 34.3 % , while it on traditional blood agar medium is 32.1 % .

结果表明，用酵母浸膏血琼脂替代巧克力琼脂培养淋球菌无统计学差异，淋球菌用平板浸润法接种比平板涂布法好。

The results indicated that there was no statistical difference when yeast extract blood agar was used instead of chocolate agar for culturing gonococcus . Inoculation of gonococcus using plate infiltration method was better than that using plate smearing method .

从送检的两只病死鸵鸟中分离到01，02两株细菌，经鉴定均为鼠伤寒沙门氏菌，其抗原模式为4，5，12：i：1，2。在血琼脂平板上可形成溶血环。

Two strains of Salmonella typhimurium were isolated and identified for two 3 ~ 4 month old ostriches died from the disease . Their antigen pattern was 4 , 5 , 12 : I : 1 , 2.They had hemolytic activities on blood agar Plate .

血消化液琼脂淋球菌选择培养基的实验研究

Experimental research on isolating gonococcus by blood-digested agar selective medium

国内外有许多犬恶丝虫病检测方法的报道，如：微丝蚴检查、血凝试验、琼脂扩散试验、补体结合试验、荧光抗体标记技术、PCR法等，但效果均不理想。

So far there are no ideal diagnostic although many detection methods were reported , such as : microfilaria examination , blood clotting tests , agar diffusion test , complement fixation test , fluorescent antibody labeling , PCR method .

副猪嗜血杆菌并得诊断主要依赖细菌分离、免疫组织化学等实验室诊断方法，而间接血凝试验、琼脂扩散试验又由于操作复杂，交叉反应严重无法基层推广使用。

The detection of Haemophilus parasuis is mainly depend on IHA , Agar Diffusion Test and IHC which have disadvantages such as cross reaction and low sensitivity .

应用马血、兔血、羊血巧克力琼脂培养基分离流感嗜血杆菌的效果评价

Effect evaluation of chocolate agar media made of horse , rabbit and sheep blood using for isolate haemophilus influenzae

结果（1）马血、兔血、羊血巧克力琼脂培养基痰培养流感嗜血杆菌的检出率分别为34.3%、35%、8.3%。

Results The isolate rates of Haemophilus influenzae in sputum by chocolate agar media made of horse , rabbit and sheep blood were 34.3 % , 35 % , 8.3 % respectively .

用MLE无血培养基与MHC（M-H血巧克力琼脂）培养基同时进行已知幽门弯曲菌（Cp）的培养及胃粘膜活检标本中Cp的分离试验。

The comparative study on MLE blood-free medium and M-H chocolate medium ( MHC ) for the isolation of Campylobacter pyloridis ( CP ) from biopsy specimens of human gastric mucosa and inoculation of CP reference strains was performed .