虎杖

虎杖中DNA裂解成分的研究

DNA Cleaving Constituents from Polygonum cuspidatum

虎杖中主要蒽醌的提取分离及HPLC分析测定

Extraction , Separation , HPLC Analysis of the Main Anthraquinones in Polygonum cuspidatum

药用植物虎杖毛状根DNA提取方法研究

Research on the Methods of DNA Extraction from Polygonum cuspidatum Hairy Roots

采用高效液相色谱法（HPLC）对虎杖中大黄素进行含量测定。

Emodin in preparation was determined by HPLC .

目的：建立消癃闭颗粒中虎杖苷含量的HPLC测定方法。

Objective : To establish a HPLC method for determination the polydatin in Xiaolongbi granules .

以液液萃取&反相高效液相色谱法研究SD大鼠静脉注射虎杖苷后药物体内分布及排泄情况。

The distribution of PD in rats was studied .

方法使用HPLC对储藏中的鲜虎杖药材样品进行含量测定。

Methods Resveratrol and piceid in fresh Polygonum cuspidatum during different storage time was determined by HPLC .

虎杖苷对小鼠肝细胞色素P450和II相酶的影响

Effects of polydatin on liver cytochrome P450 and phase ⅱ transferase in mice

虎杖苷对大鼠肾缺血-再灌注损伤NF-κB、MPO表达的影响

Effects of polydatin on expressions of NF - κ B and MPO during renal ischemia-reperfusion injury

采用乙醇提取法、水提取法及超临界CO2萃取三种方法对虎杖样品进行抽提。

The efficient of three extraction techniques , alcohol extraction method , water extraction method and supercritical CO_2 extraction were studied .

白藜芦醇、白藜芦醇苷均可降低H2O2诱导红细胞氧化性溶血率，其作用效果好于VC、VE和虎杖粗提物，抑制率具有剂量效应。

They could reduce red blood cell hemolysis induced by H2O2.The inhibition had the dose effect .

探讨了用超临界CO2萃取中药虎杖中有效成分（白藜芦醇苷、白藜芦醇和大黄素）的可行性。

The feasibility was discussed that adopting SCF-CO_2 to extract effective constituent ( piceid , resveratrol and emodin ) from Chinese herb Polygonum cuspidatum .

虎杖甙对内毒素休克大鼠肺组织中磷脂酶A2的影响。

Effect of polydatin on phospholipase A2 in lung tissues in rats with endotoxic shock .

结果在TLC鉴别中均能检出丹参、虎杖、垂盆草。

Results Radix Salviae Miltiorrhizae , Rhizoma Polygoni Cuspidati and Herba Sedi could be identified by TLC .

目的研究虎杖苷（PD）对CCl4损伤原代培养大鼠肝细胞的保护作用。

AIM To investigate the effects of polydatin ( PD ) on CCl 4 intoxicated primary cultured rat hepatocytes .

目的观察中药虎杖的活性成分白藜芦醇苷对过氧化氢（H2O2）所致肝细胞损伤的影响。

AIM To investigate the effects of polydatin on mouse hepatocyte injury induced by hydrogen peroxide ( H 2O 2 ) .

结论：LPS可促进内皮细胞ICAM-1的表达，并可被虎杖甙所抑制

CONCLUSION : The expression of ICAM-1 on endothelial cells can be promoted by LPS , and polydatin can inhibit LPS-induced ICAM-1 expression

方法采用薄层色谱法对榆柏烧伤喷雾剂中虎杖、黄柏等进行鉴别，并采用HPLC法对制剂中大黄素进行含量测定。

Methods The identification of Rhizoma Polygoni Cuspidati and Cortex Phellodendri in Yubo Shaoshang Spray were carried out by TLC . The content of emodin in the preparation was determined by HPLC .

采用电刺激大鼠颈动脉血栓形成和结扎大鼠下腔静脉方法评价虎杖苷（polydatin，PD）的抗血栓形成作用；

The methods of electrically stimulated carotid thrombosis and inferior vein ligation in rats were used to evaluate the antithrombotic effects of polydatin .

虎杖保肝降脂有效部位（EPC）制备工艺、质量控制方法及初步药效学研究

Study on the Preparing Technique , Quality Control Method and Initial Pharmacodynamics of the Effective Liver Protection and Lipid-lowering Fraction ( EPC ) in Polygonum Cuspidatum

mL-1，黄柏、虎杖的最小抑菌浓度为25.00mg。

The MIC of Phellodendron bark and giant knotweed rhizome was 25.00 mg · mL ~ ( - 1 ) .

[结论]虎杖的乙酸乙酯提取物有一定的抗炎作用，其作用机制可能是抑制炎症介质PGE2的合成、抑制细胞免疫及与垂体-肾上腺皮质系统有关。

Inhibiting synthesis of PGE2 and cellular immune function and relating with hypothalamus - pituitary - adrenocortical axis probably are the mechanisms .

虎杖提取物具有多种功能作用，主要有抗炎、预防心血管疾病等药理活性，而其主要活性成分则是白藜芦醇（resveratrol）和大黄素（emodin）。

The extracts of Polygonum have anti-inflammatory and prevention of cardiovascular disease and other pharmacological activities , including the main active ingredient is resveratrol and emodin .

结果：①在提取工艺研究中，虎杖最优提取工艺为A3B2C3D1，即用20倍量95%的乙醇提取3次，总提取时间为3h。

Results : ① The optimum extracting procedure of Polygonum cuspidatum was A_3B_2C_3D_1 , i.e. extracting with 20 times ethanol ( 95 % ) 3 times for 3 hours .

目的建立RP-HPLC测定大鼠血浆及组织样本中虎杖苷的方法，研究虎杖苷在大鼠体内的药动学行为和组织分布特点。

Objective To establish a RP-HPLC method to determine the polydatin in plasma and tissues of rats in vivo and to study its pharmacokinetic behaviour and tissue distribution characteristics .

结果：ACV及虎杖大黄素处理皮区的累积计分依次为（14.20±6.70）分，（7.00±6.16）分；

Result : The cumulative scores of the regions dealt with ACV , emodin were ( 14.20 ± 6.70 ),( 7.00 ± 6.16 ) respectively ;

结论：虎杖苷对小鼠肝微粒体Ⅰ相酶没有明显作用，但抑制II相酶UDPGT和GST。

Conclusion : Polydatin has no obvious influence on phase ⅰ transferase contents , while it has strong inhibition effects on activities of GST and UDPGT in the mice liver .

此外，虎杖治疗重症烧伤后大鼠NBT阳性率降低，其存活时间比未治疗组明显延长。

Lowering of NBT positive rate was observed in rats treat - ed with polygonum cuspidatum , and these rats lived much longer than those without the treatment .

结果正常状态下心肌细胞胞质PKC经虎杖苷（PD）刺激后活性下降（vs正常组P<0.05），但胞膜的PKC活性上升（vs正常组P<0.05）。

Results The activity of PKC of myocardial cells cytoplasm in normal situation after the cells were treated by PD decreased ( vs normal group P < 0.05 ), but membrane increased ( vs normal group P < 0.05 ) .

目的：观察虎杖甙（polydatin，PD）对大鼠心肌细胞内游离钙浓度（[Ca2+]i）的影响，以探讨PD增强心肌细胞收缩性的作用机制。

AIM : To elucidate the mechanism of polydatin ( PD ) in increasing the contractility of myocardial cells by observing the cytosolic free calcium concentration ( [ Ca 2 + ] I ) of myocardial cells of rats .