营养缺陷型

营养缺陷型出现率为1.65%。

The occurrence of the auxotroph was about 1.65 % .

筛选标记主要有营养缺陷型、药物抗性标记；

Two main select markers were auxotroph and drug resistance .

应用营养缺陷型回复突变的方法筛选枯草杆菌（Bacillussubtilis）α-淀粉酶的高产菌株

Selection of high α - amylase yeilding strains of Bacillus subtilis by auxotrophic reversion technique

啤酒酵母（saccharomycescerevisiae）营养缺陷型的筛选及原生质体融合

Auxotrophic Mutant Selection and Protoplast Fusion in Saccharomyces cerevisiae

结果：建立了一株二氨基庚二酸（DAP）营养缺陷型重组大肠杆菌DH10BΔasd。

Results : A DAP auxotrophy recombinant bacterium strain was obtained .

水稻T-DNA插入氮营养缺陷型突变体的氮营养特征

Nitrogen Nutrition Characteristics of Rice T-DNA Insertion Mutant

研究表明：UV减弱了腺嘌呤缺陷型单倍体的交配能力，而对其它营养缺陷型单倍体的交配能力无甚影响；

Further investigation demonstrated that UV light decreased the mating ability of ade auxotrophic haploids and had no significant effect on the mating ability of other auxotrophic haploids .

通过在筛选培养基中补加超高浓度维生素B2（Riboflavin）的方法（简称B2），首次成功的诱变分离到21株独立的沙门氏菌'B2营养缺陷型。

The first independent Vitamine B ? auxotrophs of Salmonella typhimurium were obtained by using selective medium containing extraordinarily high concentration of B2 ( 300 μ g / ml ) after mutagenesis .

NTG诱发产黄青霉菌产生营养缺陷型突变体的研究报道

Mutagenesis of Auxotrophic Mutants of Penicillium chrysogenum by NTG

大肠杆菌panD基因突变体为β-丙氨酸营养缺陷型。

The panD mutant of E. coli is β - alanine auxotrophy .

该18株融合子酒精发酵能力经与传统的用营养缺陷型标记筛选的23株融合子比较，表明用RAPD标记筛选到的融合子群体中，高产菌株所占的比例明显高于前者。

Compared ethanol fermentation power of these 18 fusants with 23 fusants selected by auxotrophic marker , the results demonstrated that percentage of high ethanol-yielding strains in fusants population selected by RAPD marker was significantly high than fusants population selected by auxotrophic marker .

以一株光滑球拟酵母的多重维生素营养缺陷型为研究菌株，考察了分批发酵中不同体积传氧系数（KLa）对其产丙酮酸性能的影响。

The effect of volumetric oxygen transfer coefficient ( kLa ), on the performance of producing pyruvate by a multi - vitamin - auxotrophic yeast of Torulopsis glabrata , was investigated in batch fermentation process .

定向选育氨基酸营养缺陷型苹果酒酵母突变株的研究

Study on directional breeding of aminophenol auxotroph cider yeast mutant

黑木耳激光诱变及营养缺陷型突变体筛选

Mutagenic Effects of Laser on Auricularia auricula and Selection of Auxotrophic Mutant

木耳营养缺陷型原生质体再生方式研究

Studies on the Regeneration Mode of the Protoplast of Auxotrophic Mutants of Auricularia

非营养缺陷型原生质体融合选育角蛋白酶生产菌

Breeding of keratinase - producing strain by fusion of non - auxotroph protoplast

黑木耳与毛木耳营养缺陷型诱变研究木制耳环，不含其他材料

Study on the Auxotroph of Wood Ear Fungi-Auricularia

用正交实验法筛选营养缺陷型酿酒酵母原生质体的最佳实验条件

Selection of the Optimum Conditions of Preparing an Auxotrophic Saccharomyces cerevisiae Protoplast by Orthogonal Tests

研究了4种营养缺陷型产纤维素酶菌的原生质体形成和再生条件。

The optimal conditions for protoplast isolation and regeneration of4 auxotrophic mutant strains of Trichoderma were studied .

本文采用营养缺陷型的互补进行了啤酒酵母原生质体融合产物的筛选。

Prototrophic hybrids have been obtained by the protoplast fusion between auxotrophic haploid and diploid mutants of Saccharomyces cerevisiae .

利用~（60）Co&γ射线辐照木耳担孢子，获得了9株营养缺陷型突变体。

Nine auxotrophic mutant strains were obtained by using ~ ( 60 ) Co - γ ray irradiating Auricularia basidiospores .

一项研究蛋白质互作作图的重要技术是酵母双杂系统。这项技术的应用，需要一系列酵母菌及大肠杆菌转化，而后在营养缺陷型培养基上筛选阳性克隆。

One of the most important technique which has been developed to study the mapping of protein-protein interactions is yeast two-hybrid system .

香菇原生质体诱变及营养缺陷型突变体筛选大肠杆菌乙酸代谢突变株的选育和特性研究

Induction and selection of auxotrophic mutants from the protoplasts of Lentinus edodes screening and characteristics of mutants of E. coli resistant to acetate inhibition

单核原生质体再生菌丝的菌落形态、菌丝生长速度和营养缺陷型标记等性状均稳定地保持了亲本的特性。

The characters of parents , such as morphology of colony , growth rate of mycelium and auxotroph , were maintained in protoplasted monokaryon .

尿嘧啶营养缺陷型标记具有转化效率高、容易得到转化子等优点，在丝状真菌遗传转化中受到青睐。

With high transformation frequency and easy to obtain transformants , the uridine auxotrophy is widely used in the genetic transformation systems of filamentous fungus .

以代谢控制理论为指导，确定以选育亮氨酸营养缺陷型菌株作为初筛方法，选育低产杂醇油啤酒酵母突变菌株。

Under the instruction of metabolic control theory , the screening of leucine auxotroph was determined as the method of selecting the mutant strain of low-yield fusel oil .

营养缺陷型菌株原生质体回复突变率小于10￣（－6）。

Then at the 6 point will lead to a higher pregnancy rate . The reverse mutation frequency of the protoplast of the auxotrophic mutants was less than 10 ￣( - 6 ) .

使用由亚硝基胍诱变所得到的营养缺陷型作为单倍体融合亲株的核基因标记，同时也采用线粒体球红霉素抗性突变株的小菌落形式作为融合亲株的线粒体基因标记。

The auxotrophs obtained by nitrosoguanidine treatment were used as the markers for nuclear genotype of the haploid parents , and at the same time the petite form with mitochondrial mutation in globorubermycin resistance was used as the marker for mitochondrial genotype of fusion parents .