膜片钳技术

对膜片钳技术在SDS发病机制研究中的应用前景作了展望。

The expectation about the application prospect of patch clamp technique on the mechanism of SDS were made .

方法运用膜片钳技术的细胞吸附式方法记录NMDA通道的单通道电流。

Methods Cell-attached mode patch clamp technique was employed to record the single channel current of the NMDA receptor .

全细胞膜片钳技术记录所需离子电流和AP。

The membrane currents and action potential were recorded by the whole-cell patch-clamp technique .

方法膜片钳技术全细胞记录模式记录IK·Ca。

Methods Standard whole cell recording method was used .

方法：应用膜片钳技术的内面向外式记录酶性分离的细动脉平滑肌上KATP通道。

METHODS : The currents of K ATP channels were recorded on inside-out patches of enzymatically isolated arteriolar smooth muscle cells .

方法酶消化法急性分离Wistar大鼠海马锥体神经元，采用全细胞膜片钳技术测定IK。

Methods Pyramidal neurons were enzymatically isolated from Wistar rat hippocampus .

利用全细胞膜片钳技术，我们分析了大鼠视网膜GC上NMDA受体所介导的电流。

Using whole-cell recording made from retinal slice preparations , we first characterized NMDA receptor-mediated currents on GCs .

通过使用全细胞膜片钳技术证明了在心室肌细胞上银杏苦内酯B可浓度依赖性的缩短动作电位时程，主要与增大延迟整流型钾电流有关。

Ginkgolide B may shorten the action potential duration in a concentration-dependent manner which mainly due to the increase of the delayed rectifier potassium current ( Ik ) in single ventricular myocytes by using patch-clamp techniques .

方法：采用膜片钳技术的全细胞记录方式记录人肝癌细胞在不同浓度EGF中延迟整流性K＋通道的跨膜电流。

Methods : The transmembrane current of human hepatocyte carcinoma cells was recorded by the whole cell recording technique of patch clamp in different concentration of EGF .

利用全细胞膜片钳技术，采用胶原酶B急性分离的大鼠心室肌细胞，研究了牛黄酸和二甲基-氨氯吡咪对心肌细胞膜Na+/Ca2+交换电流的影响。

Using the whole cell patch-clamp technique to study effects of taurine and 5 - ( N , N-dimethyl ) - amiloride ( DMA ) on Na + / Ca 2 + exchange current in rat ventricular myocytes .

用膜片钳技术的全细胞方式在培养大鼠脑皮层神经元胞体膜上记录到电压依赖性钙通道电流（ICa）。

The voltage dependent calcium current ( ICa ) in cultured neurons from neonatal rat cortex was recorded with whole cell patch clamp technique .

方法利用内面向外式（inside-out）膜片钳技术。

Methods Membrane patch clamp technique on inside-out membrane patch .

在培养的大鼠颈上神经节神经元上，用全细胞膜片钳技术记录γ－氨基丁酸（GABA）所激活的通道电流。

Responses of cultured rat superior cervical ganglion ( SCG ) neurons to local application of GABA were studied by using whole-cell voltage-clamp technique .

本文作者应用连细胞膜片钳技术，观察了低剂量X射线全身照射对小鼠T淋巴细胞钙激活钾通道〔K（Ca）〕的影响。

The effect of low dose X-rays on Ca2 + - activated K + channels [ K ( ca ) ] in mouse thymic lymphocytes was observed using the cell-attached configuration of patch clamp technique .

方法应用全细胞膜片钳技术在新鲜分离的大鼠背根神经节（DRG）神经元上观察AlCl3对GABA激活电流的影响。

METHODS Using whole cell patch clamp technique to investigate the effects of AlCl 3 on GABA activated currents in isolated rat DRG neurons .

应用膜片钳技术，研究了血管紧张素Ⅱ（AngⅡ）受体对心肌细胞离子通道的调节作用。

Patch clamp electrophysiologic techniques were used to study the effects of angiotensin ⅱ ( Ang ⅱ) receptor on membrane ionic channels in single guinea pig myocytes .

1个月后，用膜片钳技术研究左室非梗死区3层心肌细胞INa的改变。

One month later , I_ ( Na ) was recorded using patch-clamp techniques from non-infarcted zone of left ventricular free walls .

采用全细胞膜片钳技术研究了大鼠三叉神经节（trigeminalganglion，TG）神经元膜P2X嘌呤受体的特征。

The characteristics of purinoceptors in the membrane of rat trigeminal ganglion ( TO ) neurons were studied by using whole-cell patch clamp technique .

方法：全细胞膜片钳技术记录急性分离SD大鼠（7～10d）颈上交感神经节细胞钠通道电流；

METHODS : Whole cell patch clamp recordings were made from enzymatically isolated rat ( 7 - 10 d ) superior cervical sympathetic ganglion neurons .

方法采用标准全细胞膜片钳技术记录IL-1β对急性分离的大鼠脊髓背角神经元电压门控Na+电流的变化。

Methods The effect of IL-1 β on voltage-gated sodium currents is measured in acutely isolated sensory neurons of spinal dorsal horn of rats by means of whole-cell patch-clamp technique .

方法用标准微电极技术和全细胞膜片钳技术，分别测定豚鼠乳头状肌AP和单个心室肌细胞离子电流。

Methods Action potential of papillary muscle was studied with standard microelectrode technique and the ion currents of single ventricular myocyte in guinea pig were measured by whole cell patch clamp technique .

方法在新鲜分离的大鼠DRG神经元标本上，以全细胞膜片钳技术记录膜电流，用排管快速换液装置行胞外给药，以胞内透析技术分析信号转导途径。

Methods Rat DRG neurons were isolated mechanically and enzymatically , on which whole-cell patch clamp recording and repatch technique for intracellular dialysis were performed .

目的和方法：采用膜片钳技术之膜内面向外记录方法，在急性分离大鼠皮层神经元上，研究胞内酸碱环境改变对神经元ATP敏感钾通道的影响。

Aim and Methods : Using inside out configuration of patch clamp technique , the effects of intracellular pH on K ATP channels were studied in acutely dissociated cerebral neurons of rats .

方法分别以结扎大鼠左冠状动脉前降支所致急性心律失常模型和膜片钳技术为基础，观察M3受体的干预作用及作用机制。

Methods Using the acute ischemic model of rats and patch-clamp techniques , the effects of the M_3 receptor on the occurrence of arrhythmias and its possible mechanisms were studied .

方法采用MTF法评价HeLa细胞的存活情况，采用膜片钳技术记录HeLa细胞的电压依赖性钾电流。

Methods Viability of HeLa cells was assessed by mitochondrial dehydrogenase activity using colorimetric MTT assay and the voltage-dependent K ~ + currents were recorded by using patch-clamp technique .

目的建立与膜片钳技术相结合的单细胞逆转录聚合酶链反应（reversetranscriptasepolymerasechainreaction，RT-PCR）技术。

Objective The aim of this study is to establish the method of single cell reverse transcriptase polymerase chain reaction methods ( RT-PCR ) combining with patch clamp techniques Methods Single ventricular myocytes were isolated enzymatically using Langendorff-perfusion apparatus .

采用全细胞记录膜片钳技术，研究非洲爪蟾脑片视顶盖神经元微突触后电流（miniatureinhibitorypostsynapticcurrent，mIPSC）频率和振幅对电压依赖关系。

Experiments were performed to study the voltage-dependence of miniature inhibitory postsynaptic current ( mIPSC ) frequency and amplitude using patch-clamp technique with whole cell recording in optic tectal slices of Xenopus .

本实验的目的在于应用电生理膜片钳技术探讨银杏叶纳米制剂的神经保护作用并与普通的mGBE进行比较，进一步阐明GBE的药理作用机制。

Our aim is to explore the neuroprotective effect of GBE by means of patch clamp technique , to further study the pharmacological mechanisms of GBE .

方法：应用细胞内记录和全细胞膜片钳技术分别在大鼠脊神经节（SG）标本和新鲜分离神经元进行实验。

METHODS : Intracellu-lar recordings and whole-cell patch clamp techniques were performed on neurons in rat spinal ganglia ( SG ) preparation and neurons freshly isolated from rat SG , respectively .

全细胞膜片钳技术检测红景天苷对NMDA诱发电流（INMDA）的影响。

Whole-cell patch-clamp technique was applied to detect the effect of salidroside on NMDA-induced current ( INMDA ) . 8 .