腱细胞

腱细胞与碳纤维体外复合组织学变化的实验研究

Histological Observation of Tendon Cell Culture Combined With Carbon Fiber

电镜下胶原纤维横纹模糊消失，腱细胞膜溶解消失，核崩解，细胞器减少。

The electron microscope observation showed that the transverse striation of the collagen fibers became unclear or disappeared . The membrane of tendon cell dissolved , the cell nuclear disintegrated and the organelles reduced .

转化人胚腱细胞在PLA和PLGA表面上的粘附特性研究

A Study on Adhesive Properties of Transformed Human Embryonic Tendon Cells to PLA and PLGA

TritonX-100处理24h及36h后，可见腱细胞数量较前减少，腱膜及腱束间疏松结缔组织内细胞大部分消失；

Treatment with Triton X-100 for 24h and 36h decreased the number of tendon cells ;

毛细血管明显增生。术后4周，A、B组肌腱断端愈合情况均较好，成纤维细胞增生旺盛，向腱细胞分化。胶原纤维均匀，排列规则。

Four weeks after operation , the tendon break end in group A and B healed both well , the fibroblasts proliferate vigorously differentiation into tenocytes , collagen fibers were well distributed , the range was in regular .

培养条件下肌腱中心区域腱细胞的增殖能力

Proliferative ability of the central area tenocytes of flexor tendons in vitro

转化生长因子-β1调控腱细胞生长的机理研究

Growth Regulation of Human tenocytes by Transforming Growth Factor - β _1

人胚腱细胞转化生长因子-β受体Ⅰ、Ⅱ、Ⅲ密度分析

Assay of the density of transforming growth factor - β receptor ⅰ,ⅱ and ⅲ in human tenocytes

而在跟腱中央部的腱细胞、钙化软骨区、跟骨区以及跟骨内软骨岛等部位无阳性信号。

No positive signal was found in the central area of Achilles tendon , mineralized fibrocartilage , calcaneus and cartilage island .

目的：探索肌腱组织块培养法中腱细胞的生长规则及形态，为腱细胞培养提供一种简便、可靠的方法。

Objective : To explore the growth and morphology of the tenocytes , and provide an easy , dependable culture method .

目的研究深低温冷冻方法对肌腱细胞活性的影响，比较程序性降温和普通深低温冷冻法对腱细胞活性的影响。

To investigate the effect of cryopreservation on the viability of tenocytes , and compare the difference between normal cryopreservation and programmed cryopreservation .

目前研究表明机械负载、炎性因子影响腱细胞中基质金属蛋白的表达和活性，共同作用于腱细胞时具有协同或抑制效应。

Now researches indicated mechanical loading and cytokines can regulate MMPs expression and activity , and there is a synergetic or inhibitory effect between them .

但培养第18天和27天时，实验组腱细胞数较培养第9天少（P<0.01）。

The number of tenocytes of the experimental group were higher on the 9th day than on the 18th and 27th days after culture ( P < 0.01 ) .

兔肌腱缝合段超微结构观察结果：8周组实验趾胶原原纤维成熟排列规则，粗细均匀，纤维周期横纹清晰，腱细胞细胞质空泡减少。

Ultrastructure observation : In the 8-week group , collagen fibers in the experimental toes became mature and arranged regularly with clear striae in fiber cycle . A decrease in the number of vacuoles in cytoplasm of tendon cells was found .

虽然骨形态发生蛋白13具有近似骨形态发生蛋白12的作用，能促进成腱细胞的增殖和Ⅰ型原胶原的基因表达，但对Ⅲ型原胶原的基因表达作用不显著。

Although bone morphogenetic protein-13 has the same biological effect to bone morphogenetic protein-12 , which can increase proliferation of tendon fibroblasts and the gene expression of procollagen type I , it has not obvious effect on the expression of procollagen type ⅲ .

结论：肌腱的抗原性主要在腱细胞成分，用2%TnBP去细胞处理，可彻底去除肌腱的细胞，保留肌腱的胶原纤维结构和生物力学性能，可作为异体肌腱去抗原的一种方法。

Conclusions : The immunogenicity of tendon tissue was mainly expressed in tendon cells . Treatment of tendon with 2 % TnBP solution for 48h resulted in an acellular tendon matrix with nearly normal structure and mechanical properities . This could be a method of removal the antigenicity of tendon .

小鼠髌腱干细胞分化后的蛋白的含量相对分化前的蛋白含量明显减少，而蛋白含量的减少，说明干细胞的细胞牵引力变小。

Patellar tendon stem cells in mice after the first differentiation of the relative protein content was significantly reduced protein content , and protein content decreased , indicating that stem cells become smaller traction .

重组人骨形态发生蛋白12不但能使腱纤维原细胞的增殖，还能使Ⅰ型和Ⅲ型原胶原高表达，有助于肌腱的修复和愈合。

Recombinant human bone morphogenetic protein-12 can increase proliferation of tendon fibroblasts and the gene expressions of procollagen type ⅰ and type ⅲ, which is helpful to the repairing and healing of tendon tissues .

目的探讨兔屈趾肌腱腱鞘、腱外膜和腱内膜细胞增殖、胶原产生和转化生长因子（TGF）-β1对细胞的增殖和胶原产生的影响。

Objective To study proliferation and collagen production of tendon sheath fibroblasts , epitenon tenocytes , and endotenon tenocytes , and effects of TGF - β 1 on cell proliferation and collagen pro - duction by each of these 3 cell types in rabbit flexor tendon .

乳酸对肌腱腱鞘、腱外膜和腱内膜细胞增殖和生物学活性的影响

Effects of lactate on proliferation and biological activities of tendon sheath fibroblasts , epitenon tenocytes , and endotenon tenocytes

目的：研究在体外条件下人发角蛋白人工腱材料（HHKAT）对人胚腱细胞（HETC）的生物学作用。

AIM : To investigate the biological effects of human hair keratin artifical tendon ( HHKAT ) on human embryonic tendon cell ( HETC ) cultured in vitro .

结论：人发角蛋白人工腱植入后，自生腱的形成主要是腱细胞增殖活化，合成并分泌胶原Ⅰ型蛋白，完成肌腱的修复。

CONCLUSION : The activation , proliferation and secretion of collagen protein as well as the synthesis of collagen subtype I by tenocytes may be responsible for autologous tendon formation after human hair keratin artificial tendon implantation .

取半腱肌腱后观察其再生时间，组织学及免疫组化结果证实，再生组织的确为新生肌腱，因其具有正常的腱细胞形态和腱组织结构，且主要成分由Ⅰ型胶原组成。

Semitendinosus tendon was observed after removal of semitendinosus tendon . Histological and immunohistochemical results confirmed that the regenerated tissues were new tendons because the tissues had normal morphology and organizational structure of the tendon and were mainly composed by type ⅰ collagen .