胶原原纤维

目的：通过人羊膜与成纤维细胞的共培养，来观察成纤维细胞在HA上的生长特性，以及胶原原纤维产生情况，为使人羊膜成为组织工程皮肤的载体打下基础。

AIM : To observe the growth characteristics of fibroblasts on human amnion ( HAM ) and the occurrence of collagen fibril by co culture of fibroblasts and HAM so as to provide a basis for the HAM as the carrier of the skin tissue engineering .

猪皮组织胶原原纤维的三维结构

Three-dimensional Structure of Collagen Fibril of Pigskin

前牙本质和牙本质的胶原原纤维分粗、细两种：从前牙本质至牙本质；

There were both thin and thick collagen fibrils in the predentin and dentin .

斑块状扭结的数量与胶原原纤维的退变程度成正比。

The number of twisting collagenous fibrils closely correlated with the degree of fibrous degeneration .

胶原原纤维的特殊形态

Special Form of Collagen Fibrils

透射电镜观察到增厚的基底膜样物质是胶原原纤维编织成厚的网状结构且无细胞成分。

A thick reticular structure containing collagen fibrils and devoid of cellular elements was observed by TEM .

胶原原纤维增多。

The collagens in creased .

这些对研究体内胶原原纤维的形成过程及调节机制具有重要意义。

These data are of importance for studying the formation and regulation mechanism of collagen fibrils in vivo .

人发角蛋白人工腱诱导自体腱形成中胶原原纤维的形态学变化

Morphological changes of collagen fibrils during the formation of autogenous tendon induced by human hair keratin artificial tendon

细胞通过胞吐作用将这些物质释放到囊性腔隙中，聚合成胶原原纤维和弹性纤维，形成筛孔结构中的透明蛋白圆柱体。

These materials were released into the pseudocysts by cytoplasmic exocytosis , and formed collagenous fibrillae and elastic fibers .

而超微结构改变以软骨细胞破碎的比较多见，有的软骨细胞则增生，基质胶原原纤维明显减少。

Many broken cartilage cells could be observed and matrix collagenous fibril decreases obviously , but some cartilage cells proliferated .

电镜可见肺泡型细胞变性、坏死、脱落、数量减少，板层颗粒变性，胶原原纤维显著增生。

Electron microscope shows type ⅱ pneumocyte degeneration , necrosis , abscission , quantity decrease , lamellated granule degeneration , collagenous fibril notable hyperplasia .

结论：胶原原纤维的扭结和致密颗粒的数量是判断髓核退变程度的重要指标。

Conclusion : The ultrastructural changes such as collagenous fibril twists and dense particles are reliable indicators to identify the degree of nucleus pulposus degeneration .

结缔组织中除有成纤维细胞和颗粒细胞外还充满胶原原纤维和肌原纤维，无髓神经原纤维也较多。

All cells have secretory and absorptive functions besides4ibblasts and granular cells in connective tissue , there are myofibril , collagenous fibril and unmyelinated fibers .

碱、灰膨胀过程中猪皮胶原原纤维的形态变化及Na~+、Ca~（2+）的渗透

Morphological changes & penetration of na ~ + and ca ~ ( 2 + ) in pigskin collagen fibrils during the process of caustic soda and lime swelling

电镜下见成纤维细胞胞膜基底部向HA-ECM内伸出数个突起，细胞与羊膜基质粘附相当牢固，胞外有较多胶原原纤维堆积。

The basal membrane of fusiform fibroblasts with oval nucleus and rich in chromatin reached much protuberance into matrix of HA ECM and collagen fibril cumulating outside cells .

毛细血管内皮细胞形态结构发生改变，基膜增厚，血管周隙明显增宽，内含大量的胶原原纤维。

The structure of endothelial cells of capillaries was changed , the basal lamina became thicker and the pericapillary space became broader with a lot of collagen fibrils .

鞣酸电镜组织化学研究结果显示，在肌上皮瘤细胞周围分布有数量不等的胶原原纤维和弹性纤维团块。

The data of electron microscopic histochemical study showed that some slender collagen fibers and heavily stained elastic fibers were dispersed around myoepithelioma cells and in mucoid tissue .

方法应用扫描探针显微镜和透射电镜对猪皮组织胶原原纤维进行观察。

Methods Scanning Probe Microscope ( SPM ) and Transmission Electron Microscope ( TEM ) were employed to study the three dimensional structure of collagen fibril in pigskin tissue .

老化牙本质胶原原纤维的横纹模糊不清，数量增加，口径增粗，分布致密，走向随意；

The collagen fibrils in the dentine of aging rat enlarged in its diameter , and were denser in the pattern of their distribution and ran randomly in their direction .

术后8周，生物性人工腱膜被降解吸收部位，有较多的粗面内质网发达的成纤维细胞和直径相近的胶原原纤维，巨噬细胞减少，内吞体减少。

At the 8 weeks after the transplantation , The bioartificial aponeurosis were absorbed , where there were abundant rough endoplasmic reticulum , fibroblasts , and collagen fibrils of different sizes .

电镜结果显示Ⅴ组肺泡Ⅱ型细胞出现不同程度的线粒体肿胀、板层小体空泡化；14、21天间质中有多量胶原原纤维沉积。

The consequence of electron microscope showed swollen mitochondria , empty lamellar bodies in type ⅱ alveolar epithelial , and massive procollagen fibers deposited in the extracellular matrix in the 14th and 21st day .

兔肌腱缝合段超微结构观察结果：8周组实验趾胶原原纤维成熟排列规则，粗细均匀，纤维周期横纹清晰，腱细胞细胞质空泡减少。

Ultrastructure observation : In the 8-week group , collagen fibers in the experimental toes became mature and arranged regularly with clear striae in fiber cycle . A decrease in the number of vacuoles in cytoplasm of tendon cells was found .

大鼠软骨Ⅱ型胶原体外胶原原纤维的形成

Fibrillogenesis in vitro of type ⅱ collagen of cartilage from the rat