胶原酶溶液

脱矿后的标本加入pH7.4的胶原酶溶液，370C孵育7d。

The demineralized root samples were incubated with pH7.4 collagenase solution for 7 days at 370C .

取新西兰大白兔整段脊柱，切开纤维环取髓核组织，0.3%的Ⅱ型胶元酶胶原酶溶液中下剪碎并消化30min（37℃），所得的消化液过滤、离心获取髓核细胞。

Get New Zealand rabbits whole spine , open fiber ring for Nucleus Pulposus Tissue , 0.3 % of the type II collagen enzyme collagenase solution under shear and digestion 30 min ( 37 ° c ), the digestive fluid filtration , centrifugal Gets the nucleus pulposus cells .

从7只豚鼠前庭中取出壶腹嵴、位觉斑，放置在胶原酶溶液中孵育50min～60min后行机械分离。

The crista ampullaris and maculae acusticae were isolated from the vestibular organs of 7 guinea pigs . They were treated with collagenase for 50 or 60 minutes , then separated mechanically .

用胶原酶溶液灌注活体大白鼠肝脏得到游离肝实质细胞。

Percoll was used to separate differently aged parenchymal cells of the same rat .