脱分化

ABA对烟草叶片细胞分化和脱分化的影响

Effect of ABA on Differentiation and Dedifferentiation of Tobacco Leaf Cells

绿豆子叶脱分化过程中RNA聚合酶的制备、特性及其活性的研究

Studies of RNA polymerases isolation , purification , properties and activity in cotyledon segment of Phaseolus radiatus during Dedifferentiation

脱分化的大蒜培养细胞与分化细胞一样，具有合成和积累SOD的能力。

Such as differentiated cells in nature garlic , the undifferentiated cell in garlic callus also has the ability to synthesis and accumulate SOD .

在愈伤诱导培养过程中，使用激光共聚焦显微镜和pH计同步测定了发生脱分化过程中细胞内和培养基的pH值。

Laser scanning confocal microscope ( LSCM ) was adopted to detect the intracellular pH of two kinds of leaf explants and culture medium was detected by pH detection during the course of inducing callus .

在培养基中仅加2，4D和6BA均能较快地启动苎麻下胚轴、子叶、叶、茎外植体的脱分化。

MS medium supplemented with 2,4-D or 6-BA alone could effectively induce de-differentiation from cotyledons , hypocotyls , leaves and stem of ramie .

Expansin在成熟胚细胞分化和脱分化过程中都上调，能积极地影响细胞结构的改变。

Expansin was up-regulated during differentiation and dedifferentiation of mature embryo , which can change cell structure .

小麦成熟胚和幼胚脱分化过程中MADS及靶基因的差异表达分析

Analysis of Expression Difference of MADS and Target Genes in Dedifferentiation between Mature and Immature Wheat Embryos

魔芋叶柄薄壁细胞在脱分化形成愈伤组织过程中，其内源激素（IAA、GA3、ABA和JA）含量发生了明显的变化。

During the course of petiole parenchyma cells to form callus , the contents of endogenous hormones ( IAA , GA3 , ABA and JA ) changed obviously .

在小麦成熟胚和幼胚脱分化过程中检测到MADS盒基因分别为81和95个。

There were 81 and 95 MADS-related genes that were detected during dedifferentiation of mature and immature wheat embryos , respectively .

细胞骨架蛋白基因在分化和脱分化过程中多为上调表达，在24小时的培养过程中，成熟胚已经得到了2,4-D的刺激，并向两个不同的方向发展。

More cell skeleton protein gene expressed upward in dedifferentiation and differentiation , the mature embryo already received the 2,4-D stimulation , and developed to two different directions in 24 hour .

以3个普通小麦杂交组合的F1群体为材料，研究了小麦花药离体培养中Cu2+对花药组织脱分化和再分化特性的影响。

F_1 population materials of three cross combinations of common wheat were employed to study Cu ~ ( 2 + ) effects on dedifferentiation and re-differentiation of anther tissue of wheat in culture in vitro .

结果表明：对花药进行3C℃高温6d预处理，能显著提高小孢子脱分化能力；高温36℃预处理能诱导小孢子脱分化的启动，时间以6天最佳；

The result show that microspore dedifferentiation ability was markedly improved by using the treatment of anther under the high temperature conditions of 36 ℃ for continuous 6 days .

这些似可表明，高浓度的内源IAA可启动未成熟维管组织细胞的脱分化，而较低浓度的内源IAA流则可诱导再生形成层的发生和活动。

It further suggested that the high concentration of endogenous IAA triggered the dedifferentiation of the immature vascular tissues after girdling , and the lower concentrated IAA flow could promote the initiation and activity of the regenerated cambium .

大豆小真叶外植体在合有NAA或KT+NAA的B5培养基上，上胚轴外植体在含有KT+NAA的B5培养基上，可脱分化形成愈伤组织，并由愈伤组织直接分化成苗。

The young true leaves explant of soybean are cultured in B_5 basal medium containing NAA or KT + NAA , epicotyls explants are cultured in the B_5 medium containing KT + NAA , they are able to dedifferentiate into callus tissues , then directly develop into plantlets .

可见，MADS家族转录因子及其靶基因在成熟胚和幼胚脱分化过程中以下调为主。

So , it was clear that MADS family transcription factors and their target genes displayed down-regulatory tendency during dedifferentiation of wheat mature and immature embryos .

本文对玉米自交系在脱分化、继代及分化过程中的硝态氮代谢进行了研究，对同一基因型不同类型的愈伤组织及经干燥48h处理的愈伤组织的氮代谢进行了比较分析。

In this paper , the Nitrate metabolism of Maize inbred line was studied during the process of callus induction , subculture and regeneration respectively and the Nitrate metabolism of different types of calli of one genotype and the 48h dehydration treatment were compared .

据此我们认为在甘蔗组织培养中2.4-D可能通过调节内源激素的水平及其相互作用，引起培养物中某些生理生化过程发生改变，从而进行脱分化和愈伤组织形成。

It appeared that the sugarcane leaf segments dedifferentiation and callus formation induced by 2.4-D may be related with the changes of endogenous levels of zeatin , zeatin riboside and ABA .

组织形态学观察进一步表明，2,4-D处理条件下的部分细胞在72小时内完成脱分化和分裂过程，SAM-dependent转甲基途径可能通过两次转甲基活性的改变调控棉花细胞脱分化过程。

The histological observations further showed that some cells accomplished cellular dedifferentiation and division within 72 h in 2 , 4-D treatment , and cellular dedifferentiation might be regulated through two alterations in SAM-dependent transmethylation activity in cotton .

对相关基因的表达分析表明，GST在植物激素诱导初期的6-24小时内高度表达，PRPs在不同处理中优势表达并表现出不同的表达模式，表明它们可能与棉花细胞脱分化关系密切。

Expression analysis of related genes was performed , GST was highly expressed from 6 to 24 h after induction with phytohormone treatment . PRPs were predominantly expressed and exhibited distinct expression patterns in different treatments , suggested they are closely related to cellular dedifferentiation in cotton .

含有叶绿体的甜菊（Steviarebaudiana）愈伤组织细胞转移至新鲜培养基后，导致光合片层的逐渐减少或消失，最后叶绿体脱分化形成原质体样的结构。

Cells of Stevia rebaudiana callus tissue contain chloroplasts when cultured in a liquid medium in the light . Transfer of the callus into the fresh culture medium results in the gradual decrease or disappearance of photosynthetic lamellae and the eventual dedifferentiation of chloroplasts to form proplastid-like structures .

结果表明，脱分化始于切口周围的细胞；

The results shows that the dedifferentiation initiates from wounded cells ;

不同小麦品种幼胚脱分化特性的研究

Studies on Dedifferentiation Characteristics of Immature Embryo of Different Wheat Varieties

油菜叶肉细胞及其在脱分化和再分化中的超微结构变化

Ultrastructural Changes in Rape Mesophyll Cells during Their De-differentiation and Re-differentiation

愈伤组织的再生能力取决于脱分化的过程。

The quality of dedifferentiation determines the capacity of callus regeneration .

休眠与水稻成熟胚细胞脱分化间的关系

The Relation between Dormancy and Dedifferentiation of Mature Rice Seed

四川小麦幼胚脱分化特性研究

Study of Dedifferentiation Characteristics from Immature Wheat Embryos in Sichuan

小麦成熟胚脱分化及其植株再生研究

Studies on Dedifferentiation of Mature Wheat Embryos and Plant Regeneration

烟草叶片外植体脱分化过程的调节及其与乙烯产生的关系

Regulation of Dedifferentiation of Tobacco Leaf Explant and Its Relation to Ethylene Production

大蒜鳞芽组织脱分化条件的分析与预测

Analysis and Prediction on the Dedifferentiation Conditions of Garlic

以上四种培养基均未发生脱分化现象。

No dedifferentiation was observed on above culture medium .