脊髓灰质炎病毒

脊髓灰质炎病毒与F2噬菌体对部分消毒剂抗力的比较研究

Comparison of resistance between poliovirus and f_2 phage to some disinfectants

PCR系统制备地高辛标记的探针检测脊髓灰质炎病毒核酸

Detection of Poliovirus by Digoxigenin - labeled cDNA Probe Prepared by PCR Technique

这支美国研究团队发现了感冒病毒及更危险的脊髓灰质炎病毒赖以生存的一种蛋白质。

The US team found a protein that cold viruses and more dangerous polio-like viruses were dependent upon .

河北省格林-巴利综合征与脊髓灰质炎病毒II型关系的初步研究

The relationship between Guillain-Barre syndrome and type 2 poliomyelitis virus in Hebei province

PCR方法检测我国脊髓灰质炎病毒Ⅰ型野毒株的研究

SURVEILLANCE OF WILD POLIOVIRUS TYPE 1 IN CHINA On the Surveillance of the Media

活细胞内脊髓灰质炎病毒正链RNA可视化研究

Visualization of Poliovirus Plus-strand RNA in Living Host Cells

脊髓灰质炎病毒cDNA在哺乳动物细胞中恢复完整病毒的研究

A study on recovering intact virus in mammalian cells from polio cDNA

应用ELISA筛选脊髓灰质炎病毒单克隆抗体及其抗原分析

Application of ELISA for Screening McAb against Type ⅲ Polioviruses and Its Antigenic Analysis

利用脊髓灰质炎病毒5'NCR和RNA聚合酶基因可提高外源基因表达

Use of Poliovirus cDNA 5 ′ NCR Together with Its Polymerase Gene Can Increase Foreign Gene Expression

结论，低浓度戊二醛即可有效灭活脊髓灰质炎病毒，其灭活效果受温度和pH值的影响。

Conclusions : Glutaraldehyde can effectively inactivating poliovirus even at low concentration . Its inactivating efficacy is influenced by temperature and pH value .

在病毒感染后的不同时间，脊髓灰质炎病毒正链RNA在寄主活细胞内展示不同的分布模式。

Poliovirus plus-strand RNA was observed to display different distribution patterns in living Vero cells at different post-infection time .

将含脊髓灰质炎病毒（PV）RNA聚合酶的不同长度基因片段克隆到载体pSG5质粒上，分别构建了4个表达RNA聚合酶的质粒。

The four plasmids containing different length of RNA polymerase gene of poliovirus were constructed to express RNA polymerase .

脊髓灰质炎病毒2A蛋白在重组病毒中的功能研究

The Function of Poliovirus 2A Proteinase in the Polio-Vaccinia Recombinant Virus

人脊髓灰质炎病毒感染诱发Vero细胞凋亡的研究

Apoptosis as a cause of death in human poliovirus infected Vero cells

用免疫荧光McAb对286株脊髓灰质炎病毒标本进行定型诊断

The Application of Immunofluorescence and Neutralization McAb Tests to Typing Diagnosis of 286 Polio Strains

结论bcl-2基因家族中抗凋亡基因和促凋亡基因表达水平的差异是脊髓灰质炎病毒诱导凋亡的机制之一。

Conclusion The poliovirus-induced apoptosis might be the result of the difference between the expression levels of anti-apoptosis and pro-apoptosis genes of bcl-2 family .

运用长时间活细胞荧光影像捕获和荧光漂白恢复等技术手段，研究了脊髓灰质炎病毒正链RNA在活细胞内的行为特征。

By using living cell imaging , time-lapse monitoring and FRAP technique , the intracellular behavior of poliovirus plus-strand RNA was studied in living host cells .

脊髓灰质炎病毒C3表位和C-myc十肽表位在细菌表面的表达

Expression of C3 Epitope of Poliovirus and a Ten-peptides Epitope of C-myc on the Surface of Recombinant Bacteria

脊髓灰质炎病毒（Poliovirus）的Percoll等密度梯度离心分离

Poliovirus Separation by Centrifugation in Percoll Isopycnic Gradients

结果，甲型流感病毒的抗力，明显低于脊髓灰质炎病毒与F2噬菌体的抗力。

The results showed that the resistance of influenza virus was lower than that of poliovirus and bacteriophage .

Sabin株脊髓灰质炎病毒在Vero细胞上的传代稳定性

Stability of Poliomyelitis Virus Sabin Strain during Subculture in Vero Cells

采用ELISA的方法检测患者血清中的SARS-CoV抗体和脊髓灰质炎病毒IgG抗体。

IgG antibody to the poliovirus type 1 to 3 and the antibody to SARS-CoV were determined using enzyme linked immunosorbent assay ( ELISA ) .

脊髓灰质炎病毒正链RNA特征性的移位和重排需要寄主细胞完整的微管网络，破坏微管网络可以改变RNA的分布模式。

The translocation and rearrangement of poliovirus plus-strand RNA requires intact microtubule network of the host cell . Disruption of microtubules could alter the characteristic distribution patterns of poliovirus plus-strand RNA .

包括脊髓灰质炎病毒（PV）14株，均为疫苗相关株，其中Ⅱ型7株，占50%；

Of the isolated , there were 14 poliomyelitis viruses ( PV ) which were vaccine-related strains , including 7 strains of type II viruses ( 50 % );

方法采用悬液定量试验法，观察了大肠杆菌F2噬菌体对7种常用化学消毒剂的抵抗力，并与脊髓灰质炎病毒进行了平行比较。

Methods Suspension quantitative test was used to observe the resistance of E.coliphage F2 to7 common chemical disinfectants and to compare parallelly with poliovirus .

消毒作用60min+烘干60min，可完全灭活脊髓灰质炎病毒和完全破坏HBsAg抗原性。

Disinfection for 60 min + stoving for 60 min could completely inactivate poliovirus and completely destroy the antigenicity of HBsAg .

周围儿童血清脊髓灰质炎病毒IgG阳性率为77.4%，Ⅱ型脊髓灰质炎病毒中和抗体活性显著高于Ⅰ、Ⅲ型。

The poliovirus IgG positive rate reached 77.4 % , and the activity of neutralizing Abs against type ⅱ poliovirus was higher than against type ⅰ and type ⅲ in local children .

建立的一步PCR方法即反转录和PCR在同一管中进行，同时检测甲型肝炎和脊髓灰质炎病毒病毒RNA。

An one step procedure was developed using the PCR ( polymerase chain reaction ) for the detection of both hepatitis A virus and poliovirus RNA , in which reverse transcription and PCR were processed in one tube .

结果分离到脊髓灰质炎病毒（PV）22株，阳性率为7.53%，均为疫苗株，其中Ⅱ型病毒（PVII）12例，为优势株；

Results 22 PV ( poliovirus ) strains were isolated with 7.53 % of the positive rate . All of them were vaccine strains , of which 12 strains were PV ⅱ( predominant strains ) .

目的应用杂交瘤技术制备抗脊髓灰质炎病毒Ⅱ型Sabin株D抗原的单克隆抗体。

Objective To prepare and identify monoclonal antibody ( McAb ) against the D-antigen of poliovirus type ⅱ Sabin strain .