脂蟾毒配基

随脂蟾毒配基作用浓度和时间的延长，癌细胞核荧光强度减弱，细胞核DNA含量明显降低。

With the prolongation of time and increase of concentration of resibufogenin , fluorescence intensity of cancer cell nucleus reduced and the content of cell nucleus DNA decreased obviously .

脂蟾毒配基对人胃癌BGC-823细胞系细胞生长的干预效应

Interventional effect of resibufogenin on the cell growth of human gastric cancer BGC-823 cell line

脂蟾毒配基引起细胞线粒体膜电位下降，释放入胞质中的细胞色素C增多，促进半胱氨酸天冬氨酸蛋白酶3蛋白活化，抑制Bcl-2蛋白表达，诱导细胞凋亡。

Resibufogenin could induce the decrease of cell mitochondria membrane potential , increase cytochrome C in kytoplasm , enhance Caspase 3 activation , inhibit the expression of Bcl-2 protein and result in cell apoptosis .

脂蟾毒配基通过线粒体途径诱导人肝癌Bel-7402细胞凋亡的研究

The Study of Resibufogenin on Induction of the Apoptosis of Human Hepatocarcinoma Bel-7402 Cells by the Mitochondrial-dependent Pathway

采用细胞免疫细胞化学显色检测和WESTERNBLOTTING分析凋亡相关基因蛋白的表达来研究脂蟾毒配基对人肝癌细胞（Bel7402）凋亡的诱导作用。

The effects of resibufogenin on DNA synthesis and expression of correlative proteins in hepatoma cell apoptosis was observed by means of Western blotting and cytochemical method .

目的：观察中药蟾酥主要成分之一&脂蟾毒配基对体外培养人胃癌BGC-823细胞系细胞生长的干预及其与细胞色素C和半胱氨酸天冬氨酸蛋白酶3活化的关系。

AIM : To observe the intervention of resibufogenin , one of main components of Chinese herb venenum bufonis , on the growth of human gastric cancer BGC-823 cell line cell cultured in vitro , and the relationship with cytochrome C and Caspase-3 activation .

试验结果表明脂蟾毒配基在大鼠体内是非线性动力学消除。

The result of study show that RBG is elimination of nonlinear dynamics .

目的建立测定六神丸中脂蟾毒配基的离子抑制色谱分析法。

Purpose To establish a method for the determination of resibufogenin in Liushen pills by ion suppression chromatography .

血浆蛋白结合率的测定：采用平衡透析法测定脂蟾毒配基的血浆蛋白结合率。

Determination of plasma protein binding rate : Using the equilibrium dialysis method to determinate the plasma protein binding rate of RBG .