胚状体

激动素原来被表明抑制胚状体的形成。

Kinetin was later shown to be inhibitory for embryoid formation .

增加B族维生素和肌醇含量也有利于胚状体的诱导。

Increasing content of B vitamins and inositol could also benefit to embryoid induction .

胚状体诱导培养基为MS；

The medium of inducing embryo was MS without hormone .

通过改变培养基pH值和琼脂糖浓度，获得了由大白菜子叶-子叶柄诱导胚状体和不定芽的培养基配方。其分化生长培养基的配方分别为：MS培养基+3%蔗糖+4.5mg。

Through changing pH value and agarose concentration of culture medium , we developed a method for embryo and adventitious bud induction .

方法：以龙胆草幼嫩的茎为外植体，培养在MS培养基上，通过胚状体途径获得龙胆草组培苗芽丛。

Method : Tender stems of Gentiana scabra Bunge were cultured as explant in MS medium and clump of buds were acquired via embryoid .

酸枣（ZizyphusJujubavar.spinosusHu）组织培养中胚状体的形成

Embryoid formation of Zizyphus jujuba var. spinosus Hu in tissue culture

在增添细胞分裂素和生长素的MS或改良B5液体培养基里振荡培养，明显地促进了胚状体根的生长和茎的发育。

The root growth and the shoot development of embryoids were promoted markedly in the MS or B5 liquid medium supplemented with cytokinin and auxin by shake culture .

培养基的pH以5.8为佳，提高pH至6.8和7.8，显著不利于胚状体形成和发育；不同材料要求的蔗糖浓度、激素种类和浓度也不同。

It was harmful for embryoid development to increase pH to 6.8 ~ 7.8.The optimun sucrose concentration and types of hormone in the media varied with different materials .

结果表明，在一定范围内Co2＋促进胚状体的发生，Co2＋浓度过高效果下降。

The results showed that Co 2 + enhanced the formation of celery embryoid , but the effect decreased with further rise of its concentration .

旱芹直播土壤人工种子及胚状体分化过程中的POD、PAL活性变化

Studies on conversion of Apium graveolens artificial seeds in nonsterile soil and activities of POD and PAL during the differentiation of somatic embryo

芥菜（BrassicaJuncea）花粉胚状体与离体种胚脂肪酸的变化

Changes of Fatty Acids During the in vitro Development of Fertilized Ovary and Pollen-derived Embryoid of Brassica juncea

胚状体诱导培养30d后，大部分已经发育成熟。

After induced for 30 days , most of the embryoids were mature .

欧当归LevisticumofficinaleKoch愈伤组织诱导及小植株通过胚状体途径的再生

In Vitro Callus Induction And Plantlet Regeneration Of Levisticum Officinale Koch Through Embryoid Pathway

在胚状体发生过程中，添加Co2+显著降低培养物内源IAA和ABA水平，并提高胚状体诱导率。

Endogenous levels of IAA and ABA in the cultures were reduced and induce rates of embryoid was increased by Co 2 + during embryoid formation of celery .

在心型胚状体阶段适当降低IAA／CKs值，以来诱导正常胚状体和正常苗。

To induce the normal plant and embryoid , reducing the content of lAA / CKs after heart-shape embryoid was suggested .

本文首次报道了芥菜型油菜（BrassicaJuncea）花粉胚状体群体规模的芥酸变化的研究结果，并与种子的芥酸变化进行了对比研究。

This paper reports the experimental results on the erucic acid ( EA ) content in the pollen-derived embryoid of Brassica juncea on a vast scale of population .

低温子房共培养胚状体发生和愈伤率低，但绿苗分化率较高，施加PAA、谷氨酰胺对游离小孢子形成的胚状体有促进作用。

The optimum number of ovaries in co culture is 20 Low temperature in ovary co culture can result in low callus rate and induce high green plant rate .

接种后的马铃薯花药随着热激处理时间的延长，愈伤组织诱导率有了明显的提高，胚状体诱导率也有所增加，72h处理效果较好；

Anthers were treated with heat shock 72 h respectively , and the callus induction rate and embryoid induction rate were both increased with the increase in treatment time .

诱导培养32-38d、直径为2mm的胚状体数目达到了峰值，而绿苗分化率极低。

If embryoids were induced for 32 ~ 38 days to reach a diameter of 2 mm , a very low level of green plant regeneration was obtained .

对于花药培养和花粉培养，采用低温预处理花序（4℃下1～2d）和高温预培养（33℃下1～2d），均能促进胚状体的形成，提高产胚率。

In anther and pollen culture , using the low temperature treating anthotaxy ( 4 , 1 ~ 2day ) before being inoculated and high temperature culturing ( 33 1 ~ 2day ) after being inoculated can promote the embryogenesis and increase embryoid rate .

本研究构建了含有94个株系的DH群体3基因型是影响单倍体培养的主要因素，是决定胚状体形成的先决条件。

Consequently , a DH population which contains 94 lines was constructed in present paper . 3 Plant genotype was a main factors effecting on success in the decision to form embryoids in procedure of microspore culture .

鱼雷形胚状体或已萌发的胚状体在无激素的MS-1培养基上形成正常的再生植株，在附加激素的MS-1培养基上则产生畸型株。

Normal plantlets were regenerated from the embryoids on the hormone-free MS-1 medium , while abnormal ones formed on the MS-1 medium supplemented with hormones .

在小麦小孢子培养中，应选择诱导28-29d、直径2mm的胚状体进行分化培养。

Thus , the embryoids with a diameter of 2 mm and induction time of 28 ~ 29 days should be selected for further regeneration and differentiation in the microspore culture of wheat .

用甘蓝F1、F2和自交系S33个世代6种基因型材料进行甘蓝花药培养诱导胚状体形成影响因子研究。

Six genotypes in three generations , cabbage F_1 and F_2 and inbred S_3 were used in anther culture to study the factors affecting embryoid induction and formation of cabbage anthers in culture .

用适当浓度的植物生长抑制物质（MET、MH）处理（减数分裂期喷洒植株、低温预处理过程中浸泡花序和加入培养基中），均有利于促进胚状体的产生，提高诱导频率。

Using plant growth inhibition substance ( MET , MH ) with a propriety concentration treating donor plants in different time ( spraying plants in meiosis , dipping anthotaxy in low temperature treatment and adding them into medium ), it is helpful to increase the inductive frequencies of embryoid .

结果表明，DNA金弹与靶细胞的距离为9cm，每皿轰击一次时，胚状体诱导率可以达到1.87%。

The experiments showed that each plate of Hevea calli , which was bombarded once by gold particles coated with the plasmid and the distance between particles and target cells at 9 cm , had good results , the embryoid induction rate reaching to 1.87 % .

在培养基中添加0.1mg/ml的活性炭对红菜薹小孢子培养的胚胎发生具有较为明显的作用，能增加胚状体的产量。

Factors analysis affecting isolated microspore culture of Purple Flowering Stalk The effects of the activated charcoal on embryoid production were investigated . For most of genotypes , the ratio of embryoid production increased in the media supplemented with 0.1mg/ml activated charcoal .

棉花组织培养中胚状体发生及细胞学观察

Somatic embryogenesis in leaf culture of cotton and related cytological observation

大豆未成熟子叶诱导胚状体发生再生植株

Plant Regeneration From Immature Cotyledons Culture of Soybean Via Somatic Embryogenesis

由胚状体和愈伤组织分化芽。

The bud was differentiated from the embryoid and the callus .