羊水细胞

结果：72～100%外周血淋巴细胞中期相或间期核、60～70%羊水细胞间期核、95～99%绒毛细胞间期核显示预期信号。

Results : 72-100 % of peripheral blood lymphocyte metaphases or inter-phases , 60 - 70 % of amniocyte interphases , and 95-99 % of chorionic villus cell interphases showed expected signals .

目的：探讨羊水细胞培养及荧光原位杂交（FISH）技术在产前诊断中的实验方法及应用价值。

Objective : To study the experimental method of amniotic cell culture with fluores-cence in situ hybrydization ( FISH ) for prenatal diagnosis and its application value .

结论羊水细胞培养进行产前诊断是十分安全而可靠的。

Conclusion amniotic fluid culture for prenatal diagnosis is very safe and reliable .

结论：Ct宫颈感染与羊水细胞感染有非常显著的相关性（P<0.005）。

Conclusion : Cervical Chlamydia infection was highly related to the amniotic infection .

羊水细胞染色体高分辨G显带的初步研究

A Preliminary Study on High Resolution G-banded Chromosomes from Amniotic Fluid Cells

方法对我院优生遗传咨询门诊有产前诊断指征的孕妇采用羊水细胞培养G显带技术进行羊水细胞培养染色体核型分析。

Methods : G banding technique was used on cultured amniotic cells .

用基因释放剂从血液及羊水细胞中提取DNA直接用于PCR扩增

Preparation of genomic DNA from blood and amniotic fluid cells with ″ Genereleaser ″ for direct

方法原位法培养羊水细胞并制备染色体，G显带分析核型；

Methods Amniotic fluid cells were cultured in situ and their karyotypes were analyzed by G band .

方法：对342例具有产前诊断指征高风险孕妇抽取孕中期羊水细胞培养，制备中期细胞染色体，G显带分析。

Methods Amniocentesis was employed to detect amniotic cells collected from 342 pregnant women with high-risk prenatal diagnosis indications .

羊水细胞间期FISH结果显示，含5个杂交信号的核占所有杂交核的385%，与细胞遗传学分析的21三体核型完全一致。

Inter-FISH showed that the nucleus with five hybride signals was 38.5 % . This result was full agreement with trisomy 21 determined by cytogenetics .

聚合酶链反应的样本可以直接用溶解的羊水细胞或绒毛标本，也可以用从干血纸片抽提的DNA。

Polymerase chain reaction can take place in lysed amniotic fluid cells or chorionic villus samples without prior DNA extraction , or in the DNA extracted from dried blood spots on filter paper blotters .

进行产前诊断的57例孕妇，FISH检测其羊水细胞诊断出三例染色体异常患儿，分别是1例47，XX，+18；

Among the 57 pregnant women , three fetus with chromosome abnormality were diagnosed by FISH , the karyotypes are 47 , XX , + 18 ;

方法：用PCR扩增结合反向点杂交技术（RDB）对胎儿羊水细胞进行β-地中海贫血的基因分析。

Methods : Polymerase chain reaction and reverse dot blot ( PCR-RDB ) were used to analyze DNA of amniotic fluid cells .

羊水细胞培养失败1例，FISH检测为1正常男胎，排除了唐筛高危，与核型分析结果一致。

One failed case , at the first time of the amniotic fluid cell cultured , FISH had a successfully detection and it was a normal male fetus , which excluding tang high-risk screening .

对252例适应症对象进行改良羊水细胞培养产前诊断，取得满意的成功率，并检出DS胎儿3例。

As a technique of prenatal diagnosis , modified amniotic cell culture was successfully undertaken in 252 patients , 3 cases of them were identified with DS .

本文记述了使用改变的Shah方法，对人类羊水细胞染色体高分辨G显带获得成功。

High resolution G-banded chromosomes of the cells from human amniotic fluid were obtained with the modified Shah 's method . The procedures of this method were described in detail .

方法采用染色体特殊位点的特异性探针（LSI21）对30例羊水细胞和6例绒毛细胞标本进行未培养间期细胞荧光原位杂交。

Methods : Uncultured interphase cells including 30 cases of amniotic fluid cell and 6 cases of villous tissue were performed FISH by using specific probe ( LSI21 ) of special site on chromosome .

结论D-FISH技术不仅能检测培养的外周血标本，对未培养羊水细胞也是一种快速、简便、直观、成功率高的方法，在唐氏筛查中有较好的应用前景。

Conclusion D-FISH not only can examine cultured peripheral blood samples , but also be a quick , simple , direct method for uncultured amniotic fluid cells , with high success rate . It can be spread in the screening of down 's syndrome .

采用改良的羊水细胞培养方法，各种染色体显带技术，羊水细胞培养成功率大大提高，成功率为99.5%。进一步缩短了培养时间，平均7.6d。

Using the improved method of amniotic fluid cultivation and various techniques of chromosome banding , the rate of success was greatly increased , approaching 99.5 % , and the duration of culturing much shortened , averaging 7.6d .

用羊水细胞直接作多聚酶链反应进行基因诊断的方法

Gene analysis using amniotic fluid cells directly for polymerase chain reaction

羊水细胞培养诊断染色体病

Amniotic Fluid Cell Culture in Prenatal Diagnosis of Chromosomal Abnormalities

70例羊水细胞培养

Brief Summary of Studies on Amniotic Fluid Cell Culture from 70 Cases

羊水细胞培养与染色体制片技术探讨

On the Procedures of Amniotic - fluid Cell Culture and Chromosome Preparation

羊水细胞染色体分析在产前诊断中的应用

Applications of the amniotic cell karyotype analysis in pregnant diagnosis

提高羊水细胞染色体培养成功率研究

Investigation on improving the cultural success rate of amniotic fluid cell chromosome

羊水细胞培养技术研究

A study on the technique of amniotic fluid cell culture

羊水细胞培养预测胎儿异常112例分析

An analysis of amniotic cell culture in 112 cases to predict fetal anomalies

应用荧光原位杂交技术诊断未培养羊水细胞染色体异常

Detection of chromosomes abnormal in uncultured amniotic fluid with the fluorescent in situ hybridization

方法探索新的羊水细胞培养技术；建立收获标准及改良收获方法。

Methods : To study the new technical methods and establish the standard of harvest .

羊水细胞培养成功214例，成功率91.45%；

214 cases amniotic fluid cells culture are successful , yield rate was 91.45 % ;