细胞培养

方法采用细胞培养和限制性内切酶解分析技术观察分析细胞生长及细胞基因组DNA甲基化水平。

Methods Cell culture and methylation sensitive restriction endonuclease digestion were used .

设计：细胞培养观察及DNA检测。

DESIGN : Cell culture and DNA examination .

结论羊水细胞培养进行产前诊断是十分安全而可靠的。

Conclusion amniotic fluid culture for prenatal diagnosis is very safe and reliable .

方法绒毛细胞培养法及G显带制作染色体分析。

Methods Use villus cell culture and the chromosome with G-bands by trypsin using Giemsa .

灯盏乙素可明显改善形态学损伤，显著降低LDH释放量、细胞培养液及细胞内MDA含量，提高SOD活性。

Scutellarin can decrease LDH , MDA content and increase SOD activity significantly .

应用ELISA检测间质细胞培养基上清液中VEGF浓度？

The concentration of VEGF in the supernatant of the media was assayed by ELISA .

心肌细胞培养结果显示：H／R处理后，心肌细胞内游离钙的浓度明显高于对照组（P＜0.01）。

The intracellular free calcium concentration of cardiomyocytes in H / R group was higher compared to control ( P < 0.01 ) .

重组CHO细胞培养过程中代谢产物对细胞分泌HBsAg的影响

Effect of Metabolite on HBsAg Secretion of Recombinant CHO Cells

采用ELISA法检测各组小鼠血清、腹腔Mφ和脾细胞培养上清、肝匀浆中细胞因子的含量；

Serum cytokine levels , M φ and splenic cell culture supernatant and liver tissue extracts supernatants were measured using ELISA .

取大鼠膝关节滑膜组织体外进行原代细胞培养，用MTT比色法检测大鼠滑膜细胞的增生。

The knee synovial tissues of the rats were taken and synoviocytes were separated and cultured in vitro .

按离体细胞培养和RIA分析法研究锰对睾丸细胞分泌睾酮的影响。

The effect of Manganese on testosterone secretion of testis cells was studied by cell culture and radioimmunoassay .

SARS-CoV在Vero细胞培养中的多形性特征

Morphological Polymorphism of SARS-CoV in Vero Cell Cultures

以RP-HPLC对细胞培养液中紫杉醇的含量进行定量分析研究，并比较了2种不同的定量方法&标准曲线法和绝对分析法。

A RP HPLC is developed for the quantitative analysis of the taxol in culture solution of plant cell .

方法对320例白血病患者采用短期细胞培养法制作骨髓细胞染色体标本并观察DM表达。

Methods DM was observed in bone marrow cells after short term culture which were derived from 320 patients with verified leukemia by biopsy .

目的探索松质骨骨基质明胶（BMG）作为软骨细胞培养支架的可行性。

Objective To investigate the feasibility of cancellous BMG as scaffold for chondrocyte .

刺激剂（elicitor）在植物细胞培养次生代谢物生产中的应用

The application of elicitor in the production of second metabolites in plant cell cultures

抗CD3、PHA-P及IL-2对LAK细胞培养的生长特性，细胞表型的影响

Effect of anti-CD_3 and PHA versus IL-2 alone for initiation of LAK cells , growth characteristics and cell phenotype

应用SDS聚丙烯酰胺（SDSpage）蛋白电泳和明胶酶谱电泳分析方法，观察肝癌细胞培养上清中MMPs的分泌与活化水平的变化。

SDS-PAGE protein electrophoresis and gelatin zymography analysis were carried out to detect the changes of release and activation of MMPs in the cell culture supernatant .

刺激剂（elicitor）在植物细胞培养中被用来作为提高次生代谢物产量的手段。

Elicitor is used to improve the productivity of second metabolites in plant cell cultures frequently .

方法：在体外干细胞培养中，A组加入10%胎牛血清，B组加入10%小牛血清，C组加入10%马血清。

Method : During process of cell culture in vitro , 10 % fetal bovine serum was mixed into culture medium of A group , 10 % calf serum into B group , 10 % horse serum into C group .

MTT方法较为稳定，PDGF&BB细胞培养液具有一定活性，并能被抗人PDGF－BB多克隆抗体中和。

The cell culture liquid containing PDGF - BB was active and could be neutralized by the polyclonal antibody of human PDGF - BB .

方法：取人的髂骨松质骨进行成骨细胞培养，进行ALP染色和HE染色。

Methods : human trabecular bone of ilium was obtained to osteoblast culture , then ALP dying and HE dying were applied to identify the osteoblasts .

采用琼脂糖弥散杀菌法检测SPC-A-1细胞培养上清的抗菌活性。

The antibacterial activity of the cell culture supernatant was determined by agarose radial diffusion assay .

目的分析DispaseⅡ酶对人角膜缘干细胞培养的影响。

Objective To analyze the effect of Dispase ⅱ on human limbal stem cell culture .

采用体外人系膜细胞培养，应用细胞ELISA法检测ET-1对系膜细胞表达MMP-3及TIMP-1的影响。

Cultured human embryonic mesangial cells ( MC ) were exposed to ET-1 , the expressions of MMP-3 and TIMP-1 proteins in MCs were examined by cell ELISA .

用单细胞培养法，从人胃癌细胞系BGC-823中分离出10个克隆。

Ten cell strains were obtained from the human gastric cancer cell line BGC-823 by using the method of single cell cloning .

目的探讨用旋转细胞培养系统（RCCS）进行大动物软骨细胞扩增的可行性。

Objective To investigate the feasibility of amplifying big animal chondrocytes in vitro by using rotary cell culture system ( RCCS ) .

并且加入不同浓度的5FC后，U251/CD细胞培养液内均能检测到5FU。

HPLC analysis showed that 5-FU was detected in U251 / CD cell medium .

方法采用细胞培养和PCR两种方法检测1997年10月～1998年9月重庆医科大学附属儿童医院125例新生儿结膜炎患儿的结膜标本中的CT。

Trachomatis in neonatal conjunctival specimens . Methods A total of 125 conjunctival scrapings taken from neonates with conjunctivitis in Children ′ s Hospital during October 1997-September 1998 was tested by the McCoy cell culture and polymerase chain reaction ( PCR ) .

GEN可显著抑制致敏小鼠引流淋巴结细胞培养上清液IFN-γ、IL-2、TNF-α、IL-4、IL-10的上调（P＜0.05）。

GEN inhibited the up-regulating of IFN - γ, IL-2 , TNF - α , IL-4 , IL-10 in supernatant of cultured draining lymph node lymphocytes in sensitized mice significantly ( P 0.05 ) .