糖苷酶

唾液酸酶（sialidase/SD）是一种糖苷酶（glycosidase），功能是从糖蛋白和糖脂的非还原末端水解唾液酸（sialicacid）。

Sialidase is a kind of glycosidase , which can hydrolyzing sialic acid from non-deoxidized end glycogen and glycolipid .

多羟基生物碱（polyhydroxylatedalkaloids）作为一类重要的糖苷酶抑制剂，具有一系列重要的生物活性及潜在的药用价值。

Polyhydroxylated alkaloids as an important class of glycosidase inhibitor display a range of important biological activities and have potentials as chemotherapeutic agents .

用于B→O血型改造的不同α-半乳糖苷酶的比较

Comparison of Different α - Galactosidases Used in the B to O Blood Conversion

α-半乳糖苷酶是B→O血型改造研究中的关键工具酶。

Galactosidase is the key enzyme in the research of B to O blood conversion .

尿嘧啶DNA糖苷酶活性的电泳凝胶成像分析测定

Assay of Uracil DNA Glycosylase Activity with Gel Electrophoresis and 1D Image Analysis System

桃叶片β-半乳糖苷酶基因全长cDNA克隆及原核表达

Cloning and Expression of β - galactosidase Gene cDNA in Peach

该α-半乳糖苷酶反应的最适pH为5.0，最适温度为45℃。

The α - galactosidase has an optimum pH of 5.0 and an optimum temperature of 45 ℃ .

人溶酶体α-甘露糖苷酶cDNA的克隆与表达

Cloning and Expression of Human Lysosomal Alpha mannosidase cDNA

一个与编码大鼠ERα-甘露糖苷酶同源的人cDNA分子克隆

Cloning of a human cDNA homologous to the cDNA encoding a rat ER α mannosidase

采用Meta分析法评价α-葡萄糖苷酶抑制剂的降糖差异

Meta Analysis of Efficacy Differences of Alpha-glucosidase Inhibitors in Blood Glucose Control

结果与结论：基因重组α半乳糖苷酶的N端序列与理论序列完全一致；

Results and conclusion : The results showed that the N-terminal amino acids sequence of recombinant α - galactosidase were identical with theoretical sequence completely ;

文章通过DNA、α葡萄糖苷酶和硫酸盐还原菌等的变化，研究了贵州红枫湖沉积物中有机质的酶及微生物降解。

Enzymatic and microbial degradation of organic matter in Lake Hongfeng of Guizhou Province were studied through variations of DNA , α glucosidase and sulfate reduction bacteria .

β葡萄糖苷酶在pH为4.5～6.5之间稳定.温度30℃以上则逐渐失活。

β - glucosidase is stable between pH 4.5 ~ 6.5.It will begin to lose its activity when the temperature is higher than 30 ℃ .

系统感染TMV的番茄叶片胞外四种β－N－乙酰氨基己糖苷酶的纯化和性质

Purification and Properties of Four Forms of the Apoplastic β - N-Acetyl-D-hexosaminidase from Tomato Leaves Systemically Infected by TMV

使用InsightⅡ软件中的Modeler模块以1UOK为模板对实验使用的α-葡萄糖苷酶进行同源模建。

The Modeler module of the Insight II software was used to build the a-glucosidase model .

采用响应面分析，对酶解温度、pH、β-葡萄糖苷酶/滤纸酶的比进行研究。

The response surface methodology was applied to temperature of hydrolysis , pH value of hydrolysis and he ratio of β - glucosidase activity and filter paper activity .

HCV多表位抗原基因与β-半乳糖苷酶基因融合表达、产物纯化及酶活性分析

Expression and Purification of Multi-epitopes Antigen Gene of HCV Fused to β - Galactosidase Gene and Analysis of its Enzyme Activity

目的：构建葡萄糖苷酶Ⅰ（glucosidaseⅠ，GluⅠ）特异性siRNA真核表达载体。

Objective : To construct eukaryotic expression vector of siRNA specific for rat glucosidase ⅰ .

短双歧杆菌（Bifidobacteriumbreve203）α-D-半乳糖苷酶的诱导合成及部分酶性质研究

Induction and characterization of α - d-galactosidase ( ec 3.2.1.22 ) from Bifidobacterium breve 203

目的观察培养人视网膜色素上皮（RPE）细胞中复制衰老相关的β半乳糖苷酶活性。

Objective To observe the replicative senescence-related beta-galactosidase activity in cultured human retinal pigment epithelium ( RPE ) cells .

重组酶的最适温度为50℃，最适pH为5.4。培养基中β-葡萄糖苷酶活性最高可达47U/mL。

The optimum temperature of the recombinant β - Glucosidase was 50 ℃, and the optimum pH was 5.4.The activity of β - Glucosidase could reach to 47U / mL in the culture medium .

影响Kluyveromycesfragilisβ-D-半乳糖苷酶催化合成低聚半乳糖的因素

Factors Affecting Galacto-oligosaccharide Synthesis by Kluyveromyces Fragilis β - D-galactosidase

11个Fabry病家系的α-半乳糖苷酶A活性及GLA基因检测

Study of alpha-galactosidase A activity detection and gene survey in 11 Chinese pedigrees with Fabry disease

半乳糖苷酶检测结果发现，GCSF受体在酵母细胞中不呈现转录激活功能，然而EPO受体则出现转录激活功能。

The β - galactosidase colony-lift filter assay demonstrated that G-CSF receptor alone had no activity of transcriptional activation , while the EPO receptor alone could activate transcription .

血清αL岩藻糖苷酶对肝癌的意义

Clinical significance of serum α _L_ fucosidase in liver cancer

产β-半乳糖苷酶酵母菌株15D产酶条件研究

Study on the conditions for the production of β - galactosidase by yeast 15D

结果表明，HBsAg／45肽免疫组IL－2水平明显高于β－半乳糖苷酶／45肽免疫组。

The results showed that the level of IL-2 in the HBsAg / 45-mer immune group was much higher than in the β - galactosidase / 45-mer immune group .

常用的研究方法有易错PCR，DNAshuffling，定点诱变等等；如姜世民等利用异源DNA同源重组体外定向进化大肠杆菌的p-半乳糖苷酶。

Commonly used research methods are error-prone PCR , DNA Shuffling and site-directed mutagenesis and so on ; such as Jiang Shimin maked heterologous DNA homologous recombination in vitro evolution of E. coli P-galactosidase .

β-半乳糖苷酶的EA、ED这种α-互补性还被用于分子生物学、蛋白质相互作用的监控、表达免疫分析等方面的研究。

The a - complementation reaction of & - galactosidase EA and ED was also used in DNA cloning , protein protein interactions monitoring and expressing immunoassays studying .

了解尿白蛋白和尿N-乙酰-β-D氨基葡萄糖苷酶（NAG）对系统性红斑狼疮患者早期肾损害的诊断价值。

To study the diagnostic value of urinary albumin and urinary N-acetylglucosamine polyase ( NAG ) to the early stage renal impairment .